Biosensing for nanospace synapses and its application to measurements of brain functions
| Project/Area Number |
24350032
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NIWANO Michio 東北大学, 電気通信研究所, 教授 (20134075)
KIMURA Yasuo 東北大学, 電気通信研究所, 准教授 (40312673)
AONUMA Yuki 東北大学, 電気通信研究所, 助教 (80582262)
YAMAMOTO Hideaki 東北大学, 学際科学フロンティア研究所, 助教 (10552036)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGAWARA Masao 日本大学, 文理学部, 教授 (50002176)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
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| Keywords | バイオセンサ / 神経伝達物質 / シナプス / イオンチャネル / 脂質二分子膜 |
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| Outline of Final Research Achievements |
We have developed biosensors for bio-nanospace “synapses” and investigated their application to measurements in brain. We combined amperometric glutamate microsensor and recording of field excitatory postsynaptic potentials (fEPSPs) as a new method for analyzing relation between presynaptic and postsynaptic activities. Although it was found that electric stimuli and evoked fEPSPs induced a capacitive transient current at the microsensor, we proposed a method to extract a faradaic signal from the observed currents. Then we applied this method to long-term potentiation (LTP) in the hippocampus. It was found that the level of the extracellular L-glutamate during tetanus stimulation was significantly related to LTP induction. Thus, our simultaneous recording method for pre- and post-synaptic activities can be a useful tool for neurosciences. We also investigated efficient construction of lipid bilayer-based sensor utilizing ion channel as a sensory element for neurotransmitters.
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Report
(4 results)
Research Products
(60 results)
