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Low-temperature biochemical experiments by utilizing supercooled-confined water within silica mesopore

Research Project

Project/Area Number 24350034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Analytical chemistry
Research InstitutionIbaraki University

Principal Investigator

Yamaguchi Akira  茨城大学, 理学部, 准教授 (10359531)

Co-Investigator(Kenkyū-buntansha) ITOH TETSUJI  産総研, 主任研究員 (70392587)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywordsメソ細孔 / 過冷却 / タンパク質 / DNA
Outline of Final Research Achievements

Cryobiology is one of important approach to study structure and function of biomacromolecules such as DNA, RNA, and proteins. The purpose of this study is application of supercooled confined water within mesoporous silica for low-temperature biochemical experiments.
Time-resolved fluorescence study revealed good molecular diffusion within the supercooled confined water. In study on duplex formation of short-DNA fragments, it was revealed that the silica pore with supercooled water could be used to achieve the duplex formation, which could not be occurred in bulk water system. On the other hand, we found that freezing/melting behaviors of the pore water were strongly depended on the amount of protein confined inside the pore. The morphology of protein inside the pore was successfully observed by neutron scattering. These results indicated availability of mesoporous silica for the low-temperature biochemical experiments of biomacromolecules.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (57 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 5 results,  Acknowledgement Compliant: 5 results) Presentation (42 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results) Remarks (4 results)

  • [Journal Article] Collimated microfiber spectroscopy for optical characterization of disordered porous anodic alumina2016

    • Author(s)
      S. Yoneda, S. Kimura, A. Yamaguchi, D. Kimura, Y. Ohkoshi, S. Yamanaka, H. Usami
    • Journal Title

      Appl. Phys. Exp.

      Volume: 9 Issue: 2 Pages: 022503-022503

    • DOI

      10.7567/apex.9.022503

    • NAID

      210000137780

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermodynamics of Complexation between Thiourea-Based Receptor and Acetate in Water/Acetonitrile Mixture2016

    • Author(s)
      T. Suzuki, Y. Shibuya, T. Sato, S. Nishizawa, I. Sato, A. Yamaguchi
    • Journal Title

      Anal. Sci.

      Volume: in press

    • NAID

      130005163085

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural Stability of Light-harvesting Protein LH2 Adsorbed on Mesoporous Silica Supports2015

    • Author(s)
      Y. Shibuya, T. Itoh, S. Matsuura, A. Yamaguchi
    • Journal Title

      Analytical Sciences

      Volume: 31 Issue: 10 Pages: 1069-1074

    • DOI

      10.2116/analsci.31.1069

    • NAID

      130005102896

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Trinucleotide duplex formation inside a confined nanospace under supercooled conditions2014

    • Author(s)
      Hiroyuki Arafune, Akira Yamaguchi, Manato Namekawa, Yusuke Sato, Tetsuji Itoh, Ryoko Yoshida, Norio Teramae
    • Journal Title

      Nature Commun.

      Volume: 5 Issue: 1 Pages: 5151-5151

    • DOI

      10.1038/ncomms6151

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Electrochemical enzymatic biosensor with longterm stability using hybrid mesoporous membrane2014

    • Author(s)
      Tetsuji Itoh, Takeshi Shimomura, Akari Hayashi, Akira Yamaguchi, Norio Teramae, Masatoshi Ono, Tatuo Tsunoda, Fukio Mizukami Galen D. Stucky, Taka-aki Hanaoka
    • Journal Title

      Analyst

      Volume: 139 Issue: 18 Pages: 4654-4660

    • DOI

      10.1039/c4an00975d

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inclusion Complexation of γ-Cyclodextrin and Coumarin Dye inside Alumina Nanopores Over a Temperature Range of 303 - 223 K2013

    • Author(s)
      Akira Yamaguchi, Tetsuya Denda
    • Journal Title

      J. Phys. Chem. C

      Volume: 117 Issue: 34 Pages: 17567-17573

    • DOI

      10.1021/jp404504d

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural Regulation of Mesoporous Silica and Characterization of the Microenvironment Inside a Silica Mesopore2013

    • Author(s)
      山口央,渋屋祐太,成澤淳,滑川真人,菅原大輝,荒船博之,寺前紀夫
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 62 Issue: 7 Pages: 581-588

    • DOI

      10.2116/bunsekikagaku.62.581

    • NAID

      10031178753

    • ISSN
      0525-1931
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Encapsulation of PEG-modified Myoglobin in Hydrophobic Mesoporous Silica as Studied by Optical Waveguide Spectroscopy2013

    • Author(s)
      H. Arafune, A. Yamaguchi, K. Hotta, N. Teramae
    • Journal Title

      Analytical Sciences

      Volume: 29 Issue: 2 Pages: 187-192

    • DOI

      10.2116/analsci.29.187

    • NAID

      10031156854

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] メソポーラスシリカ細孔構造制御と細孔内環境の評価2013

    • Author(s)
      山口央, 渋屋祐太, 成澤淳, 滑川真人, 菅原大輝, 荒船博之, 寺前紀夫
    • Journal Title

      分析化学

      Volume: (掲載確定)

    • NAID

      10031178753

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microviscosity of Supercooled Water Confined within Aminopropyl-modified Mesoporous Silica as Studied by Time-resolved Fluorescence Spectroscopy2012

    • Author(s)
      A. Yamaguchi, M. Namekawa, T. Itoh, N. Teramae
    • Journal Title

      Analytical Sciences

      Volume: 28 Issue: 11 Pages: 1065-1070

    • DOI

      10.2116/analsci.28.1065

    • NAID

      130004442086

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deposition of Polyelectrolyte Multilayer Film on a Nanoporous Alumina Membrane for Stable Label-Free Optical Biosensing2012

    • Author(s)
      K. Hotta, A. Yamaguchi, N. Teramae
    • Journal Title

      J. Phys. Chem. C

      Volume: 116 Issue: 44 Pages: 23533-23539

    • DOI

      10.1021/jp308724m

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Optical Waveguide Spectroscopy for Study of Biological Events inside Inorganic Nanopores2015

    • Author(s)
      Akira Yamaguchi
    • Organizer
      PACIFICHEM 2015
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 炭素被覆メソポーラスシリカを用いた直接電子移動型酵素電極の開発2015

    • Author(s)
      渋屋祐太, 伊藤徹二, 干川康人,京谷隆,山口央
    • Organizer
      第12回茨城地区分析技術交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 中性子散乱によるシリカメソ細孔内ミオグロビンの構造評価の検討2015

    • Author(s)
      木島惇,渋屋祐太,山口央,阿久津和宏,岩瀬裕希,福嶋喜章
    • Organizer
      第12回茨城地区分析技術交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 無機多孔質材料に吸着した周辺光捕集複合体LH2の熱安定性2015

    • Author(s)
      上野涼太郎, 渋屋祐太,山口央
    • Organizer
      第26回日本化学会関東支部茨城地区研究交流会
    • Place of Presentation
      日立市多賀市民プラザ
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] チオ尿素型アニオンレセプターと酢酸イオンの錯形成におよぼす水和の影響2015

    • Author(s)
      鈴木貴矢,山口央
    • Organizer
      第26回日本化学会関東支部茨城地区研究交流会
    • Place of Presentation
      日立市多賀市民プラザ
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 炭素被覆メソポーラスシリカを用いた新規酵素電極の開発2015

    • Author(s)
      渋屋祐太, 伊藤徹二, 干川康人,京谷隆,山口央
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学伊都キャンパス
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] チオ尿素型アニオンレセプターと酢酸イオンの錯形成の温度依存性2015

    • Author(s)
      鈴木貴矢,山口央
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学伊都キャンパス
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 中性子散乱を利用したシリカメソ細孔内ミオグロビンの構造評価の検討2015

    • Author(s)
      木島惇,渋屋祐太,山口央,阿久津和宏,岩瀬裕希,福嶋喜章
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学伊都キャンパス
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 水/アセトニトリル混合系におけるチオ尿素型レセプターの水素結合型錯体形成の温度依存性2015

    • Author(s)
      鈴木貴矢,山口央
    • Organizer
      平成27年度関東支部分析若手交流会
    • Place of Presentation
      晴海グランドホテル
    • Year and Date
      2015-06-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] シリカナノ細孔内における3 塩基DNA 二重鎖形成の配列依存性2014

    • Author(s)
      吉田量子、山口央
    • Organizer
      第11回茨城地区分析技術交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Year and Date
      2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] シリカ細孔内における(CCG)n ヘアピン型2 次構造の安定性2014

    • Author(s)
      那須一啓, 渋屋祐太,山口央
    • Organizer
      第11回茨城地区分析技術交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Year and Date
      2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] アルミナナノ細孔内における4 塩基DNA の二重鎖の形成2014

    • Author(s)
      木村彩歩、佐藤翔平、山口央
    • Organizer
      第11回茨城地区分析技術交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Year and Date
      2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] アルミナナノ細孔を利用したバイオセンサー2014

    • Author(s)
      山口 央
    • Organizer
      第31回金属のアノード酸化皮膜の機能化部会(ARS)足柄コンファレンス
    • Place of Presentation
      いこいの村あしがら
    • Year and Date
      2014-11-20 – 2014-11-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 蛍光共鳴エネルギー移動を利用した一塩基多型検出の検討2014

    • Author(s)
      茂木えりか, 宇田麻希, 樫村成輝, 山口央
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学東広島キャンパス
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 無機ナノ細孔内に吸着した膜タンパク質LH2の安定性2014

    • Author(s)
      渋屋祐太, 山口央
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学東広島キャンパス
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] アルミナ表面に吸着した光捕集複合体の熱安定性2014

    • Author(s)
      上野涼太郎,渋屋祐太,山口央
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学東広島キャンパス
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 無機ナノ細孔内での膜タンパク質の安定性評価2014

    • Author(s)
      渋屋祐太
    • Organizer
      平成26年度東日本分析若手交流会
    • Place of Presentation
      鶴岡メタボロームクラスター
    • Year and Date
      2014-07-11 – 2014-07-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] アルミナ表面に吸着した光捕集複合体LH2の熱安定性2014

    • Author(s)
      上野涼太郎,渋屋祐太,山口央
    • Organizer
      平成26年度東日本分析若手交流会
    • Place of Presentation
      鶴岡メタボロームクラスター
    • Year and Date
      2014-07-11 – 2014-07-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] アルミナナノ細孔内における短鎖DNAの低温ハイブリダイゼーション2014

    • Author(s)
      山口央, 佐藤翔平
    • Organizer
      第74回分析化学討論会
    • Place of Presentation
      日本大学工学部
    • Year and Date
      2014-05-24 – 2014-05-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] ナノポーラス光導波路分光法によるナノポーラス膜における固定化酵素の解析2013

    • Author(s)
      荒船博之、堀田一海、山口央、伊藤徹二、寺前紀夫
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス、矢上キャンパス
    • Year and Date
      2013-03-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] 光導波路分光法によるPEG修飾ミオグロビンの吸着挙動解析2013

    • Author(s)
      山口 央
    • Organizer
      第23回日本MRS年次大会
    • Place of Presentation
      横浜市開港記念会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] ナノポーラス構造に基づくバイオセンシング2013

    • Author(s)
      山口 央
    • Organizer
      2013年真空・表面科学合同講演会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] メソポーラスシリカに吸着した光捕集複合体LH2の立体構造2013

    • Author(s)
      渋屋祐太, 山口央
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      近畿大学東大阪キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] アルミナナノ細孔内における脱塩基部位含有DNA二重鎖と小分子リガンドの低温錯形成挙動2013

    • Author(s)
      宇田麻希, 樫村成輝, 山口央
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      近畿大学東大阪キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Low-temperature complexation of a ligand with abasic site-containing DNA inside alumina nanopores2013

    • Author(s)
      Maki Uda, Shigeki Kashimura, Akira Yamaguchi
    • Organizer
      RSC Tokyo International Conference 2013
    • Place of Presentation
      幕張メッセ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Fabrication of mesoporous silica tubes inside columnar alumina pores2013

    • Author(s)
      Ryotaro Ueno, Kazuyoshi Nasu, Akira Yamaguchi
    • Organizer
      RSC Tokyo International Conference 2013
    • Place of Presentation
      幕張メッセ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structure of Light-Harvesting Protein LH2 Adsorbed on Mesoporous Silica2013

    • Author(s)
      Yuuta Shibuya, Akira Yamaguchi
    • Organizer
      The Twelfth Asian Conference on Analytical Sciences (ASIANALYSIS XII)
    • Place of Presentation
      Maidashi campus of Kyushu University
    • Related Report
      2013 Annual Research Report
  • [Presentation] アルミナナノ細孔内におけるクマリンーシクロデキストリン包接錯体平衡2013

    • Author(s)
      山口央, 傳田哲矢
    • Organizer
      第73回分析化学討論会
    • Place of Presentation
      北海道大学函館キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 温度によるメソポーラスシリカ細孔径の制御2012

    • Author(s)
      成澤淳, 渋屋裕太, 山口央
    • Organizer
      第23回日本化学会関東支部茨城地区研究交流会
    • Place of Presentation
      日立シビックセンター
    • Year and Date
      2012-11-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of hybridization behavior of short oligonucleotides in supercooled water confined to silica mesopores2012

    • Author(s)
      Hiroyuki ARAFUNE, Manato NAMEKAWA, Akira YAMAGUCHI, and Norio TERAMAE
    • Organizer
      平成24年度日本分光学会国際シンポジウム・年次講演会
    • Place of Presentation
      東京工業大学百年記念館
    • Year and Date
      2012-11-27
    • Related Report
      2012 Annual Research Report
  • [Presentation] シリカメソ細孔内に閉じ込められたタンパク質構造の細孔径と温度依存性2012

    • Author(s)
      渋屋祐太, 山口央
    • Organizer
      第10回茨城地区分析技術交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Year and Date
      2012-11-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] ナノ空間での短鎖DNA会合挙動の評価2012

    • Author(s)
      樫村成輝, 山口央
    • Organizer
      第10回茨城地区分析技術交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Year and Date
      2012-11-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] 多孔質ナノ材料の分析化学などへの応用について2012

    • Author(s)
      山口央
    • Organizer
      富山県衛生研究所合同セミナー
    • Place of Presentation
      富山県衛生研究所(招待講演)
    • Year and Date
      2012-09-18
    • Related Report
      2012 Annual Research Report
  • [Presentation] アルミナ細孔内部におけるクマリン色素とシクロデキストリンの包摂錯体平衡の温度依存性2012

    • Author(s)
      傳田哲矢, 山口央
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学角間キャンパス
    • Year and Date
      2012-09-18
    • Related Report
      2012 Annual Research Report
  • [Presentation] シリカメソ細孔内に閉じ込められた光捕集タンパク質LH2の構造評価2012

    • Author(s)
      渋屋祐太, 山口央
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学角間キャンパス
    • Year and Date
      2012-09-18
    • Related Report
      2012 Annual Research Report
  • [Presentation] 陽極酸化法により形成した多孔質チタニア/チタン積層膜の電極特性評価2012

    • Author(s)
      山本良明, 山口央
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学角間キャンパス
    • Year and Date
      2012-09-18
    • Related Report
      2012 Annual Research Report
  • [Presentation] Hybridization of short oligonucleotides 1n supercooled water confined to silica mesopores2012

    • Author(s)
      Hiroyuki ARAFUNE, ManatoNAMEKAWA, Akira YAMAGUCHI, and Norio TERAMAE
    • Organizer
      JASIS (Japan Analytical Scientific Instruments Show)
    • Place of Presentation
      幕張メッセ
    • Year and Date
      2012-09-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] 陽極酸化法による多孔質チタニア/チタン積層膜の作製2012

    • Author(s)
      山本良明, 山口央
    • Organizer
      平成24年度日本分析化学会東日本分析若手交流会
    • Place of Presentation
      いこいの村涸沼
    • Year and Date
      2012-06-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] シリカメソ細孔内部におけるポルフィリン誘導体の酸解離反応

    • Author(s)
      成澤淳, 渋屋祐太, 山口央
    • Organizer
      第10回茨城地区分析技術交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Related Report
      2013 Annual Research Report
  • [Presentation] 円筒状アルミナナノ細孔でのイオン伝導度変化を利用したバイオセンシング法の検討

    • Author(s)
      山本良明, 山口央
    • Organizer
      第10回茨城地区分析技術交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Related Report
      2013 Annual Research Report
  • [Presentation] アルミナナノ細孔内における短鎖DNA二重鎖形成

    • Author(s)
      佐藤翔平, 山口央
    • Organizer
      第10回茨城地区分析技術交流会
    • Place of Presentation
      いばらき量子ビーム研究センター
    • Related Report
      2013 Annual Research Report
  • [Presentation] Fabrication of Hybrid Mesoporous Membrane for Bioanalysis

    • Author(s)
      Akira Yamaguchi
    • Organizer
      9th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCOO13)
    • Place of Presentation
      Ritsumeikan University
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks] 茨城大学理学部山口研究室HP

    • URL

      http://anal.sci.ibaraki.ac.jp/yama/yamalab.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 茨城大学理学部山口研究室

    • URL

      http://anal.sci.ibaraki.ac.jp/yama/yamalab.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 茨城大学理学部山口研究室HP

    • URL

      http://anal.sci.ibaraki.ac.jp/yama/yamalab.html

    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://anal.sci.ibaraki.ac.jp/yama/yamalab.html

    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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