Low-temperature biochemical experiments by utilizing supercooled-confined water within silica mesopore
| Project/Area Number |
24350034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITOH TETSUJI 産総研, 主任研究員 (70392587)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | メソ細孔 / 過冷却 / タンパク質 / DNA |
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| Outline of Final Research Achievements |
Cryobiology is one of important approach to study structure and function of biomacromolecules such as DNA, RNA, and proteins. The purpose of this study is application of supercooled confined water within mesoporous silica for low-temperature biochemical experiments.
Time-resolved fluorescence study revealed good molecular diffusion within the supercooled confined water. In study on duplex formation of short-DNA fragments, it was revealed that the silica pore with supercooled water could be used to achieve the duplex formation, which could not be occurred in bulk water system. On the other hand, we found that freezing/melting behaviors of the pore water were strongly depended on the amount of protein confined inside the pore. The morphology of protein inside the pore was successfully observed by neutron scattering. These results indicated availability of mesoporous silica for the low-temperature biochemical experiments of biomacromolecules.
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Report
(5 results)
Research Products
(57 results)
