Development of theree-dimentional cell culture chip for drug screening
| Project/Area Number |
24350037
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Shuji 東京大学, 大学院総合文化研究科, 准教授 (90447318)
TOMITA Shunsuke 独立行政法人産業総合技術総合研究所, バイオメディカル研究部門, 研究員 (50726817)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 幹細胞 / マイクロパタン / 三次元培養 / 分泌液 / secretome / 合成高分子 / 酵素 / 分化 / 細胞チップ / マイクロ / 判別分析 / 高分子 / ポリｲｵﾝコンプレックス / バイオセンサー / 肝毒性評価 / 創薬 / バイオ界面 |
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| Outline of Final Research Achievements |
The effects of three-dimensional environment in culture for ADSCs were researched on microfabricated surface. We successfully accomplished the 3-D construction of ADSCs (ADSC spheroid) with high viability and differentiate the ADSC spheroid into osteoblasts efficiently. The Live/Dead staining assay revealed that cells in the ADSC spheroid maintained high viability for at least 28 days in culture. After the additional cultivation of ADSC spheroid in osteogenic differentiation medium for 14 days, the ADSC spheroids were characterized the extent of differentiation by specific staining for osteoblasts and RT-PCR. We have also applied a PIC sensor array to recognize “secretomic signatures” of culture supernatants for markerless and noninvasive identification of differentiated MSCs using only a standard microplate reader. Theseis technology and the findings in this study could provide an interesting new approach to the construction of bone tissues for regenerative medicine.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Long-term survival and functional maintenance of hepatocytes by using a microfabricated cellarray2012
Author(s)
Ikeda, Y., Jomura, T., Horiuchi, U., Saeki, J., Yoshimoto. K. Ikeya, T., Nagasaki, Y.,
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Journal Title
Colloids and Surfaces B : Biointerfaces
Volume: 97
Pages: 97-100
Related Report
Peer Reviewed
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[Presentation] 3D Cell Co-culture System on Hydrogel Micro-Patterned Surface Fabricated by Photolithography2012
Author(s)
K., Yoshimoto, R., Kojima, E., Takahashi, M., Ichino, H., Miyoshi, and Yukio Nagasaki
Organizer
International Conference of Photopolymer Science and Technology (ICPST)
Place of Presentation
千葉大学(千葉)(依頼講演)
Year and Date
2012-06-27
Related Report
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