Split molecular catalyst and its analytical application as a signal amplifier
Project/Area Number |
24350040
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Analytical chemistry
|
Research Institution | Kumamoto University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
SAKURAI Toshihiko 鳥取大学, 工学部, 准教授 (10332868)
IMAHORI Tatsushi 東京理科大学, 工学部, 講師 (90433515)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
|
Keywords | DNA / DNAコンジュゲート / 機能性核酸 / アプタマー / DNAzyme / 金属イオン / シクロデキストリン / 触媒反応 / コンジュゲート / 協同性 / 加水分解 / 金属錯体 / 有機分子触媒 / 再構成触媒 / バイナリープローブ / マイケル付加 / スプリット有機分子触媒 / シグナル増幅反応 / Michael付加 / Aldol反応 / プレートリーダー |
Outline of Final Research Achievements |
Chemically engineered DNAs, global conformation of which can be modulated in response to specific stimuli, could be allosteric functional DNAs or work as a modulator of the functional nucleic acids such as DNAzymes and aptamers. We showed that two terpyridines built in the DNA backbone form a stable intramolecular 1 : 2 complex, [M(terpy)2]2+, with divalent transition metal ions. Upon complexation, the DNA conjugates adopt a Ω-shape structure in which two distal sequences located outside the terpyridines connect with each other to form a continuous segment with a specific structure or sequence. Such DNA structure is globally controlled by local metal complexation events that can be rationally designed based on general coordination chemistry. This method is regarded as metal ion-directed dynamic sequence edition or DNA splicing. Split DNAzymes with peroxidase-like activity can thus be regulated by several transition metal ions through sequence edition techniques based on the Ω-motif.
|
Report
(4 results)
Research Products
(29 results)
-
-
-
-
[Journal Article] In Situ Oxygenous Functionalization of a Graphite Electrode for Enhanced Affinity toward Charged Species and a Reduced Graphene Oxide Mediator2014
Author(s)
M. R. Karim, Y. Ikeda, T. Ide, S. Sugito, K. Toda, YM. R. Karim, Y. Ikeda, T. Ide, S. Sugimoto, K. Toda, Y. Kitamura, T. Ihara, T. Matsui, T. Tani. Kitamura, T. Ihara, T. Matsui, T. Taniguchi, M. Koinuma, Y. Matsumoto, S. Hayami
-
Journal Title
New Journal of Chemistry
Volume: 38
Issue: 5
Pages: 2120-2127
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Rational design for cooperative recognition of specific nucleobases using β-cyclodextrin-modified DNAs and fluorescent ligands on DNA and RNA scaffolds2013
Author(s)
A. Futamura, A. Uemura, T. Imoto, Y. Kitamura, H. Matsuura, C. -X. Wang, T. Ichihashi, Y. Sato, N. Teramae, S. Nishizawa, T. Ihara
-
Journal Title
Chemistry, A European Journal
Volume: 19
Issue: 32
Pages: 10526-10535
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-