The shortest synthetic route of polymeric glycomaterials through a one-pot reaction system based on organic reaction and enzymatic reaction
| Project/Area Number |
24350052
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Polymer chemistry
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
Shoda Shin-ichiro 東北大学, 工学(系)研究科(研究院), 教授 (10143364)
|
|---|
| Project Period (FY) |
2012-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
|
|---|
| Keywords | 化学酵素合成 / 糖タンパク質 / ヒトミルクオリゴ糖 / 糖オキサゾリン / 脱水縮合剤 / 熱応答性高分子 / 糖転移反応 / 糖加水分解酵素 / 化学酵素合成法 / トラスツズマブ / モノクローナル抗体 / 糖鎖 / 抗体医薬品 / 変異型酵素 / ラクトＮ－ビオース / ラクトＮ－テトラオース / ポリアクリルアミド / キトオリゴ糖 / 配糖化 / 酵素触媒 / ワンポット化 / SGオリゴ糖 / CDMBI |
|---|
| Outline of Final Research Achievements |
A basic research on the development of one-pot glycosylation based on chemical reaction and enzymatic reaction has been carried out. An oligosaccharide having an N-acetylglucosamine moiety at the reducing end was converted to the corresponding sugar oxazoline derivative in more than 95% yields by using 2-chloroimidazolinium salt or 2-chlorobenzimidazolium salt as dehydrating agents. As a model compound for controlling enzyme activities, several thermosensitive glycopolymers based on poly(N-isopropylacrylamide) having a chitooligosaccharide was prepared. Based on these results,glycoproteins having a definite oligosaccharide as well as human milk oligosaccharides have successfully been prepared in good yields.
|
Report
(5 results)
Research Products
(31 results)
-
[Journal Article] Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) using a chemoenzymatic approach have different affinities for FcgammaRIIIa and variable antibody-dependent cellular cytotoxicity activities2016
Author(s)
M. Kurogochi, M. Mori, K. Osumi, M. Tojino, S. Sugawara, S. Takashima, Y. Hirose, W. Tsukimura, M. Mizuno, J. Amano, A. Matsuda, M. Tomita, A. Takayanagi, S. Shoda, T. Shirai
-
Journal Title
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] 4,6-Dimethoxy-1,3,5-triazin-2-ylβ-D-glycosaminides : Novel Substrates for Transglycosylation Reaction Catalyzed by Exo-β-D-glucosaminidase from Amycolatopsis orientalis2011
Author(s)
Tomonari Tanaka, Tomonori Wada, Masato Noguchi, Masaki Ishihara, Atsushi Kobayashi, Takayuki Ohnuma, Tamo Fukamizo, Ryszard Brzezinski, Shin-ichiro Shoda
-
Journal Title
Journal of Carbohydrate Chemistry
Volume: 31
Issue: 8
Pages: 634-646
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
