| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Outline of Final Research Achievements |
A basic research on the development of one-pot glycosylation based on chemical reaction and enzymatic reaction has been carried out. An oligosaccharide having an N-acetylglucosamine moiety at the reducing end was converted to the corresponding sugar oxazoline derivative in more than 95% yields by using 2-chloroimidazolinium salt or 2-chlorobenzimidazolium salt as dehydrating agents. As a model compound for controlling enzyme activities, several thermosensitive glycopolymers based on poly(N-isopropylacrylamide) having a chitooligosaccharide was prepared. Based on these results,glycoproteins having a definite oligosaccharide as well as human milk oligosaccharides have successfully been prepared in good yields.
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