Analysis of RNA editing and chemical modification at the single molecule level
| Project/Area Number |
24350084
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
Kawai Kiyohiko 大阪大学, 産業科学研究所, 准教授 (50314422)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | 遺伝子診断 / RNA / エピジェネティクス / １分子計測(SMD) / DNA / 1分子計測(SMD) |
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| Outline of Final Research Achievements |
A new detection method was developed for detection of RNA at the single-molecule level. We foucused on that the charge transfer rate through DNA is strongly affected by the DNA sequence and chemical modification of nucleobases. The charge-transfer dynamics in DNA/RNA hybrid was measured as the length of the duration of the off time of blinking of the fluorescence. This enabled the detection of target sequence, allowing for sample sizes as small as 5 fmol (< 0.2 nM, 20 uL). The target can be analyzed under conditions of the automatic measurement of more than 100 samples within 3 h, which makes possible the high-throughput screening needed in RNA diagnosis.
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Report
(5 results)
Research Products
(43 results)
