UV resonance Raman investigation on detection of higher order structural changes of heme proteins and elucidation of functional regulation mechanism

• Project/Area Number: 24350086
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: University of Hyogo
• Principal Investigator: KITAGAWA Teizo 兵庫県立大学, 生命理学研究科, 名誉教授 (40029955)
• Co-Investigator(Kenkyū-buntansha): OGURA Takashi 兵庫県立大学, 生命理学研究科, 教授 (70183770) ; NAGATOMO Shigenori 筑波大学, 数理物質科学研究科, 講師 (80373190)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 生物物理 / 蛋白質 / シグナル伝達 / 生体分子 / 分子認識 / 振動分光 / 共鳴ラマン散乱

Outline of Final Research Achievements

Cavity mutant human Hb, which lacks the Fe-His bond in either the α or β subunit but can bind four O2 molecules, was prepared. Its oxygen binding curve was determined, and higher order structural changes upon O2 binding were investigated with 1H NMR, V- and UV-resonance Raman, and nearUV CD spectra. Spectroscopic measurements revealed that the quaternary structure change did not occur upon ligand binding. The absence of Fe-His bond in α froze Hb in T while that of β raised the affinity of α. Resonance Raman spectra of NO sensor protein, mammalian sGC and its model protein, bacterial H-NOX, were investigated.

Research Products

• [Journal Article] Infrared and Raman spectroscopic investigation of the reaction mechanism of cytochrome c oxidase (2015)
  • Author(s): Satoru Nakashima, Takashi Ogura, Teizo Kitagawa
  • Journal Title: Biochimica et Biophysica Acta Volume: 1847 Issue: 1 Pages: 86-97
  • DOI: 10.1016/j.bbabio.2014.08.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Formation and Characterization of a Reactive Chromium(V)-Oxo Complex: A Mechanistic Insight into Hydrogen-Atom Transfer Reactions (2015)
  • Author(s): H. Kotani, S. Kaida, T. Ishizuka, M. Sakaguchi, T. Ogura, Y. Shiota, K. Yoshizawa, T. Kojima
  • Journal Title: Chemical Science Volume: 6 Issue: 2 Pages: 945-955
  • DOI: 10.1039/c4sc02285h
  • NAID: 120007130469
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Higdla is a oositive regulaator of cytochrome c oxidase (2015)
  • Author(s): T. Hayashi, Y. Asano, Y. Shintani, H. Aoyama, H. Kioka, O. Tsukamoto, M. Hikita, K. Shinzawa-Itoh, K. Takajuji, S. Higo, H. Kato, S. Yamazaki, K. Matsuoka, A. Nakano, H. Asanuma, M. Asakura, T. Minamino, Y. Goto, T. Ogura, M. Kitakaze, I. Komuro, Y. Sakata, T. Tsukihara, S. Yoshikawa, S. Takashima
  • Journal Title: Proc. Nat. Acad. Sci. U.S.A. Volume: 112 Issue: 5 Pages: 1553-1558
  • DOI: 10.1073/pnas.1419767112
  • Peer Reviewed / Open Access
• [Journal Article] Resonance Raman spectroscopy with chemical state selectivity on histidine and acetamide using synchrotro radiation (2015)
  • Author(s): M. Saito, F. D. Amico, G. Camisasca, F. Benchivonga, R. Cucini, A. Gossini, E. Principi, T. Ogura, and C. Masciovecchio
  • Journal Title: Bu.. Chem. Soc. Jpn Volume: 88 Issue: 4 Pages: 591-596
  • DOI: 10.1246/bcsj.20140367
  • NAID: 130005064988
  • Peer Reviewed
• [Journal Article] Involvement of propionate side chians of the hee in circular dichroism of myoglobin: experimental and theoretical analyses (2015)
  • Author(s): M. Nagai, C. Kobayashi, Y. Nagai, K. Imai, N. Mizusawa, H. Sakurai, S. Neya, M. Kayanuma, M. Shoji and S. Nagatomo
  • Journal Title: J. Phys. Chem. B Volume: 119 Pages: 1275-1287
• [Journal Article] Picobiology revealed by vibrational spectroscopy (2014)
  • Author(s): Teizo Kitagawa
  • Journal Title: Journal Spectroscopic Society of Japan Volume: 63 Pages: 49-61
  • NAID: 40020077942
  • Peer Reviewed
• [Journal Article] Highly Reactive Nonheme Iron(III)-Iodosylarene Complexes in Alkane Hydroxylation and Sulfoxidation Reactions (2014)
  • Author(s): S. Hong, B. Wang, M. S. Seo, Y. M. Lee, M. J. Kim, H. Kim, T. Ogura, R. Garcia-Serres, M. Clemancey, J. M. Latour, W. Nam
  • Journal Title: Angewant Chemie Intl. Edi. Volume: 53 Issue: 25 Pages: 6388-6392
  • DOI: 10.1002/anie.201402537
  • Peer Reviewed
• [Journal Article] Determination of damage-free crystal structure of an X-ray sensitive protein using an XFEL (2014)
  • Author(s): Hirata K, Shinzawa-Itoh K, Yano N, Takemura S, Kato K, Hatanaka M, Muramoto K, Kawahara T, Tsukihara T, Yamashita E, Tono K, Ueno G, Hikima T, Murakami H, Inubushi Y, Yabashi M, Ishikawa T, Yamamoto M, Ogura T, Sugimoto H, Shen J-R, Yoshikawa S, Ago H
  • Journal Title: Nature Methods Volume: 11 Issue: 7 Pages: 734-736
  • DOI: 10.1038/nmeth.2962
  • Peer Reviewed
• [Journal Article] Effect of the electron density of the heme Fe atom o the Fe-Histidine coordination bond in deoxy myoglobin (2014)
  • Author(s): R. Nishimura, T. Shibata, H. Tai, I. Ishigami, S. Yanagisawa, T. Ogura, S. Neya, A. Suzuki, Y. Yamamoto
  • Journal Title: Bull. Chem. Soc. Jpn. Volume: 87 Issue: 8 Pages: 905-911
  • DOI: 10.1246/bcsj.20130331
  • NAID: 130004153305
  • Peer Reviewed
• [Journal Article] Geometric control of nuclearity in copper(I)/dioxygen chemistry (2014)
  • Author(s): T. Abe, Y. Morimoto, T. Tano, K. Mieda, H. Sugimotok N. Fujieda, T. Ogura, and S. Itoh
  • Journal Title: Inorganic Chemistry Volume: 53 Issue: 16 Pages: 8786-8794
  • DOI: 10.1021/ic501461n
  • Peer Reviewed
• [Journal Article] Electronic control of ligand-binding preference of a myoglobin mutant (2014)
  • Author(s): R. Nishimura, D. Matsumoto, T. Shibata, S. Yanagisawa, T. Ogura, H. Tai, T. Matsuo, S. Hirota, S. Neya, A. Suzuki, Y. Yamamoto
  • Journal Title: Inorganic Chemistry Volume: 53 Issue: 17 Pages: 9156-9165
  • DOI: 10.1021/ic5011924
  • Peer Reviewed
• [Journal Article] Influence of ligand flexibility on the electronic structure of oxidized Ni(III)-phenoxide complexes (2014)
  • Author(s): M. Kawai, T. Yamaguchi, S. Masaoka, F. Tani, T. Kohzuma, L. Chiang, T. Storr, K. Mieda, T. Ogura, r. K. Szilagyi, and Y. Shimazaki
  • Journal Title: Inorganic Chemistry Volume: 53 Issue: 19 Pages: 10195-10202
  • DOI: 10.1021/ic501181k
  • Peer Reviewed
• [Journal Article] Effects of proton motive force on the structure and dynamics of bovine cytochrome c oxidase in phospholipid visicles (2014)
  • Author(s): T. Nomura, S. Yanagisawa, K. Shinzawa-Itoh, S. Yoshikawa, and T. Ogura
  • Journal Title: Biochemistry Volume: 53 Issue: 40 Pages: 6382-6391
  • DOI: 10.1021/bi501022j
  • Peer Reviewed
• [Journal Article] Interplay between phase transition of DPPC bilayer and photoisomerization of doped stilbene molecule (2014)
  • Author(s): K. Nakazawa, M.Hishida, S. Nagatoo, Y. Yamamura, and K. Saito
  • Journal Title: Chemistry Letters Volume: 43 Pages: 1352-1354
  • Peer Reviewed
• [Journal Article] Recentant nematic phase in 4-alkyl-4'-cyanobiphenyl(nCB) binary mixtures (2014)
  • Author(s): S. Fujimura, Y. Yamamura, M. Hishida, S. Nagatomo, and K. Saito
  • Journal Title: Liquid Crystals Volume: 41 Pages: 927-932
  • Peer Reviewed
• [Journal Article] Copper complexes supported by an N2S-tridentate ligand as active site models of copper monooxygenases (2014)
  • Author(s): Shinobu Itoh, et al.
  • Journal Title: Dalton Trans. Volume: 42 Issue: 12 Pages: 4871-4877
  • DOI: 10.1039/c3dt52952e
  • Peer Reviewed
• [Journal Article] Roles of Carboxylate Donors in O-O Bond Scission of Peroxodiiron(III) to High-Spin Oxodiiron(IV) with a New Carboxylate-Containing Dinucleating Ligand (2014)
  • Author(s): M. Kodera, T. Tsuji, T. Yasunaga, Y. Kawahara, T. Hirano, Y. Hitomi, T. Nomura, T. Ogura, Y. Kobayashi, Sajith P. K., Y. Shiota, and K. Yoshizawa
  • Journal Title: Chem. Sci. Volume: 5 Issue: 6 Pages: 2282-2292
  • DOI: 10.1039/c3sc51541a
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effective Pumping Proton Collection Facilitated by a Copper Site (CuB) of Bovine Heart Cytochrome c Oxidase, Revealed by a Newly Developed Time-resolved Infrared System (2013)
  • Author(s): Minoru Kubo, Satoru Nakashima, Satoru Yamaguchi, Takashi Ogura, Masao Mochizuki, Jiyoung Kang, Masaru Tateno, Kyoko Shinzawa-Itoh, Koji Kato and Shinya Yoshikawa
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 42 Pages: 30259-30269
  • DOI: 10.1074/jbc.m113.473983
  • Peer Reviewed
• [Journal Article] Redox Properties of Mononuclear Copper(II)-Superoxide Complex (2013)
  • Author(s): Shinobu Itoh
  • Journal Title: Inorg. Chem. Volume: 52 Issue: 18 Pages: 10431-10437
  • DOI: 10.1021/ic401261z
  • Peer Reviewed
• [Journal Article] Near-UV Circular dichroism and UV resonance Raman spectra of tryptophan residues as a structural marker of proteins. (2013)
  • Author(s): Biswajit Pal, Katsuhiro Tanaka, Shigeo Takenaka, and Teizo Kitagawa
  • Journal Title: J. Porphyrins Phthalocyanines Volume: １７ Issue: 03 Pages: 240-246
  • DOI: 10.1142/s1088424613500375
  • Peer Reviewed
• [Journal Article] Near-UV Circular dichroism and UV resonance Raman spectra of tryptophan residues as a structural marker of proteins. (2013)
  • Author(s): Shigenori Nagatomo, Masako Nagai, Takashi Ogura, and Teizo Kitagawa
  • Journal Title: J. Physical Chemistry B Volume: 117 Issue: 32 Pages: 9343-9363
  • DOI: 10.1021/jp404685x
  • Peer Reviewed
• [Journal Article] Mononuclear manganese-peroxo and bis(μ-oxo)dimanganese complexes bearing a common N-methylated macrocyclic ligand (2013)
  • Author(s): Hyeona Kang, Jaeheung Cho, Kyung-Bin Cho, Takashi Nomura, Takashi Ogura, Wonwoo Nam
  • Journal Title: Chemistry Volume: 19 Issue: 42 Pages: 14119-14125
  • DOI: 10.1002/chem.201301641
  • Peer Reviewed
• [Journal Article] Electronic Control of Discrimination between O2 and CO in Myoglobin Lacking the Distal Histidine Residue (2013)
  • Author(s): R. Nishimura, T. Shibata, I. Ishigami, T. Ogura, H. Tai, S. Nagao, T. Matsuo, S Hirota, O. Shoji, Y. Watanabe, K. Imai, S. Neya, A. Suzuki, Y. Yamamoto
  • Journal Title: Inorg. Chem. Volume: 53 Issue: 2 Pages: 1091-1099
  • DOI: 10.1021/ic402625s
  • Peer Reviewed
• [Journal Article] Oxo-sulfido- and oxo-selenido-molybdenum(VI) complexes possessing dithiolene ligand related to the active sites of hydroxylases of molybdoenzymes: Low temperature preparation and characterisation (2013)
  • Author(s): Shinobu Itoh, et al.
  • Journal Title: Chem. Commun. Volume: 49 Issue: 39 Pages: 4358-4360
  • DOI: 10.1039/c2cc35345h
  • Peer Reviewed / Open Access
• [Journal Article] A new series of bis(ene-1,2-dithiolato)tungsten(IV), -(V), -(VI) complexes as reaction centre models of tungsten enzymes: preparation, crystal structures and spectroscopic properties (2013)
  • Author(s): Shinobu Itoh, et al.
  • Journal Title: Dalton Trans. Volume: 42 Issue: 9 Pages: 3059-3070
  • DOI: 10.1039/c2dt32179c
  • Peer Reviewed / Open Access
• [Journal Article] Resonance Raman spectral properties of FMN of bovine heart NADH:ubiquinoneoxidoreductase suggesting a mechanism for the prevention of spontaneous production of reactive oxygen species (2013)
  • Author(s): Hikita M, Shinzawa-Itoh K, Moriyama M, Ogura T, Kihira K, Yoshikawa S
  • Journal Title: Biochemistry Volume: 52(1) Issue: 1 Pages: 98-104
  • DOI: 10.1021/bi3011972
  • Peer Reviewed
• [Journal Article] Identification of Hydrogen-Bonded Oligomers in Associating Liquid by 'H NMR : 1-Phenyl-1-cyclohexanol (2013)
  • Author(s): Shigenori Nagatomo, Megumi Nobuhira, Yasuhisa Yamamura, Masato Sumita, and Kazuya Saito
  • Journal Title: Bull. Chem. Soc. Jpn. Volume: (印刷中) Issue: 5 Pages: 569-576
  • DOI: 10.1246/bcsj.20120322
  • NAID: 10031167848
  • Peer Reviewed
• [Journal Article] Radical localization in a series of symmetric Ni^<II> complexes with oxidized salen ligands (2013)
  • Author(s): Linus Chiang, Amelie Kochem, Olivier Jarjayes, Tim J. Dunn, Herve Vezin, Miyuki Sakaguchi, Takashi Ogura, Maylis Orio, Yuichi Shimazaki, Fabrice Thomas and Tim Storr
  • Journal Title: Chemistry-A European Journal Volume: 18 Issue: 44 Pages: 14117-14127
  • DOI: 10.1002/chem.201201410
  • Peer Reviewed
• [Journal Article] Interaction of dimeric horse cytochrome c with cyanide ion (2013)
  • Author(s): Ari Dwi Nugraheni, Satoshi Nagao, Sachiko Yanagisawa, Takashi Ogura and Shun Hirota
  • Journal Title: J Biol Inorg Chem Volume: 18 Issue: 3 Pages: 383-390
  • DOI: 10.1007/s00775-013-0982-8
  • NAID: 120005716871
  • Peer Reviewed
• [Journal Article] Relationship between the Electron Density of the Heme Fe Atom and the Vibrational Frequencies of the Fe-Bound Carbon Monoxide in Myoglobin (2013)
  • Author(s): R. Nishimura, T. Shibata, H. Tai, I. Ishigami, T. Ogura, S. Nagao, T. Matsuo, S. Hirota, K. Imai, S. Neya, A. Suzuki, and Y, Yamamoto
  • Journal Title: Inorg. Chem. Volume: 52 Issue: 6 Pages: 3349-3355
  • DOI: 10.1021/ic3028447
  • Peer Reviewed
• [Journal Article] Resonance Raman Study on Indoleamine 2,3-Dioxygenase: Control of Reactivity by Substrate-Binding (2013)
  • Author(s): Sachiko Yanagisawa, Masayuki Hara, Hiroshi Sugimoto, Yoshitsugu Shiro and Takashi Ogura
  • Journal Title: Chem. Phys. Volume: in press Pages: 178-183
  • DOI: 10.1016/j.chemphys.2013.02.032
  • Peer Reviewed
• [Journal Article] Effects of the bHLH domain on axial coordination of heme in the PAS-A domain of neuronal PAS domain protein 2 (NPAS2) : Conversion from His119/Cys170 coordination to His119/His171 coordination (2012)
  • Author(s): Takeshi Uchida, Ikuko Sagami, Toru Shimizu, Koichiro Ishimori, and Teizo Kitagawa
  • Journal Title: J. Inorg. Biochem. Volume: 108 Pages: 188-195
  • DOI: 10.1016/j.jinorgbio.2011.12.005
  • Peer Reviewed
• [Journal Article] Near-UV circular dichroism and UV resonance Raman spectra of individual trytophan residues in human hemoglobin and their changes upon the quaternary structure transition (2012)
  • Author(s): Masako Nagai, Shigenori Nagatomo, Yukifumi Nagai, Kenichi Ohkubo, Kiyohiro Imai, and Teizo Kitagawa
  • Journal Title: Biochemistry Volume: 51 Issue: 30 Pages: 5932-5941
  • DOI: 10.1021/bi300347x
  • Peer Reviewed
• [Journal Article] An intermediate conformational state during ligand binding to cytochrome c oxidase detected by time-resolved resonance Raman analyses of heme peripheral groups (2012)
  • Author(s): Izumi Ishigami, Takeshi Nishigaki, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Satoru Nakashima and Takashi Ogura
  • Journal Title: Chem. Lett. Volume: 2 Issue: 2 Pages: 178-180
  • DOI: 10.1246/cl.2012.178
  • NAID: 130004426495
  • Peer Reviewed
• [Journal Article] Site-specific protein dynamics in communication pathway from sensor to signaling domain of oxygen sensor protein, HemAT-Bs : time-resolved ultraviolet resonance Raman study (2012)
  • Author(s): S. El-Mashtoly, M. Kubo, Y. Gu, H. Sawai, S. Nakashima, T. Ogura, S. Aono, T. Kitagawa
  • Journal Title: J. Biol. Chem Volume: 287 Issue: 24 Pages: 19973-19984
  • DOI: 10.1074/jbc.m112.357855
  • Peer Reviewed
• [Journal Article] Copper(I)-Dioxygen Reactivity in a Sterically Demanding Tripodal Tetradentate tren Ligand. Formation and Reactivity of a Mononuclear Copper(II) End-on Superoxo Complex (2012)
  • Author(s): Shinobu Itoh, et al.
  • Journal Title: Eur. J. Inorg. Chem. Volume: 2012 Issue: 29 Pages: 4574-4578
  • DOI: 10.1002/ejic.201200177
  • Peer Reviewed
• [Journal Article] Non-innocent ligand behaviour of a bimetallic Cu complex employing a bridging catecholate (2012)
  • Author(s): Tim J. Dunn, Linus Chiang, Caterina F. Ramogida, Mike I. Webb, Didier Savard, Miyuki Sakaguchi, Takashi Ogura, Yuichi Shimazaki and Tim Storr
  • Journal Title: Dalton Transactions Volume: 41 Issue: 26 Pages: 7905-7914
  • DOI: 10.1039/c2dt30444a
  • Peer Reviewed
• [Journal Article] [Fe^<IV>=O(TBC)(CH3CN)]^<2+> : Comparative Reactivity of Iron(IV)-Oxo Species with Constrained Equatorial Cyclam Ligation (2012)
  • Author(s): Samuel A. Wilson, Junying Chen, Seungwoo Hong, Yong-Min Lee, Martin Clemancey, Ricardo Garcia-Serres, Takashi Nomura, Takashi Ogura, Jean-Marc Latour, Britt Hedman, Keith O. Hodgson, Wonwoo Nam, and Edward I. Solomon
  • Journal Title: J. Am. Chem. Soc. Volume: 134 Issue: 28 Pages: 11791-11806
  • DOI: 10.1021/ja3046298
  • Peer Reviewed
• [Journal Article] Tuning the geometries of a de novo blue copper protein by axial interactions (2012)
  • Author(s): Daigo Shiga, Yusuke Hamano, Misato Kamei, Yasuhiro Funahashi, Hideki Masuda, Miyuki Sakaguchi, Takashi Ogura and Toshiki Tanaka
  • Journal Title: Journal of Biological Inorganic Chemistry Volume: 17 Issue: 7 Pages: 1025-1031
  • DOI: 10.1007/s00775-012-0916-x
  • Peer Reviewed
• [Journal Article] Effect of the Axial Ligand on the Reactivity of the Oxoiron(IV) Porphyrin p-Cation Radical Complex: Higher Stabilization of the Product State Relative to the Reactant State (2012)
  • Author(s): A. Takahashi, D. Yamaki, K. Ikemuma, T. Kurahashi, T. Ogura, M. Hada, and H. Fujii
  • Journal Title: Inorg. Chem. Volume: 51(13) Issue: 13 Pages: 7296-7305
  • DOI: 10.1021/ic3006597
  • Peer Reviewed
• [Journal Article] Reversible O-O Bond Scission of Peroxodiiron(III) to High-Spin Oxodiiron(IV) in Dioxygen Activation of a Diiron Center with a Bis-tpa Dinucleating Ligand as a Soluble Methane Monooxygenase Model (2012)
  • Author(s): Masahito Kodera, Yuka Kawahara, Yutaka Hitomi, Takashi Nomura, Takashi Ogura, and Yoshio Kobayashi
  • Journal Title: J. Am. Chem. Soc. Volume: 134 Issue: 32 Pages: 13236-13239
  • DOI: 10.1021/ja306089q
  • Peer Reviewed
• [Journal Article] Mechanistic insights into catalytic oxidations of organic compounds by ruthenium (IV)-oxo complexes with pyridylamine ligands (2012)
  • Author(s): Shingo Ohzu, Tomoya Ishizuka, Yuichiro Hirai, Hua Jiang, Miyuki Sakaguchi, Takashi Ogura, Shunichi Fukuzumi, Takahiko Koj ima
  • Journal Title: Chemical Science Volume: 3 Issue: 12 Pages: 3421-3431
  • DOI: 10.1039/c2sc21195e
  • Peer Reviewed
• [Journal Article] Chromium(IV)-peroxo complex formation and its nitric oxide dioxygenase reactivity (2012)
  • Author(s): Yokoyama, A., Han, J. E., Cho, J., Kubo, M., Ogura, T., Siegler, M. A., Karlin, K. D., Nam, W
  • Journal Title: J. Am. Chem. Soc Volume: Vol. 134 Issue: 37 Pages: 15269-15272
  • DOI: 10.1021/ja307384e
  • Peer Reviewed
• [Journal Article] Synthesis, Characterization, and Reactivity of Hypochlorito-Iron(III) Porphyrin Complexe (2012)
  • Author(s): Zhiqi Cong, Sachiko Yanagisawa, Takuya Kurahashi, Takashi Ogura, Satoru Nakashima, and Hiroshi Fujii
  • Journal Title: J. Am. Chem. Soc Volume: 134 Issue: 51 Pages: 20617-20620
  • DOI: 10.1021/ja3108774
  • Peer Reviewed
• [Journal Article] Resonance Raman Application in Investigations of Cytochrome c Oxidase (2012)
  • Author(s): Takashi Ogura
  • Journal Title: Biochim. Biophys. Acta Volume: 1817 Issue: 4 Pages: 575-578
  • DOI: 10.1016/j.bbabio.2011.11.016
  • Peer Reviewed
• [Presentation] 金属中心－タンパク質間の情報伝達ダイナミクス：赤外分光法による検出 (2015)
  • Author(s): 小倉尚志
  • Organizer: 分子研研究会「生物無機化学の最先端の今後の展望」
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2015-01-06 – 2015-01-07
  • Invited
• [Presentation] Different roles of the fe-His bonds of the a and b subunits toward the quaternary structure change of human hemoglobin; Oxygen binding properties, resonance Raman, 1H NMR, and near-UV CD studies of cavity mutants (2014)
  • Author(s): Teizo Kitagawa
  • Organizer: 7th Asian Biological Inorganic Chemistry Conference
  • Place of Presentation: Gold Coast, Australia
  • Year and Date: 2014-11-30 – 2014-12-05
  • Invited
• [Presentation] Vibrational spectroscopic investigation of molecular mechanisms of aerobic respiration (2014)
  • Author(s): T. Ogura
  • Organizer: Advances in Live Single-cell Thermal Imaging and Manipulation
  • Place of Presentation: 沖縄科学技術大学院大学シーサイドハウス（沖縄県国頭郡恩納村）
  • Year and Date: 2014-11-10 – 2014-11-12
  • Invited
• [Presentation] Function and structure of mutant hemoglobins with proximal histidine replaced by glycine in either a or b subunits (2014)
  • Author(s): S. Nagatomo, Y. Nagai, Y. Aki, H. Sakurai, N. Maruyama, K. Imai, N. Mizusawa, T. Ogura, T. Kitagawa and m. Nagai
  • Organizer: 日本生物物理学会第５２回年会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Resonance Raman investigation of structural changes of heme of sGC caused by substrate, ligand and effectors (2014)
  • Author(s): Teizo Kitagawa
  • Organizer: 8th International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: istanbul, Turkey
  • Year and Date: 2014-06-22 – 2014-06-27
  • Invited
• [Presentation] Cooperative structural dynamics of proton pumping elements in cytochrome c oxidase as studied by onnovative infrared spectroscopy (2014)
  • Author(s): T. Ogura, S. Nakashima, M. Kubo, S.Yamaguchi, M. Mochizuki, K. Shinzawa-itoh, S. Yoshikawa
  • Organizer: 8th International Conference on Porphyrins and Phthalocyanines (ICPP-8)
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2014-06-22 – 2014-06-27
  • Invited
• [Presentation] Visible and UV resonance Raman study on indoleamine 2,3-dioxygenase (2014)
  • Author(s): S. Yanagasawa, M. Hara, K. E. Kayama, H. Sugimoto, Y. Shiro, and T. Ogura
  • Organizer: 8th International Conference on Porphyrins and Phthalocyanines (ICPP-8)
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2014-06-22 – 2014-06-27
  • Invited
• [Presentation] チトクロムc酸化酵素の酸化還元反応の時間分解赤外吸収測定を目的とした酸素肺フローシステムの開発 (2014)
  • Author(s): 西口達人, 引田理英, 新澤-伊藤恭子, 吉川信也, 中島 聡, 小倉尚志
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学 東山キャンパス（愛知県）
• [Presentation] 時間分解振動分光法によるチトクローム酸化酵素の反応初期過程 (2014)
  • Author(s): 中島 聡, 久保 稔, 石上 泉, 新澤-伊藤恭子, 吉川信也, 小倉尚志
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学 東山キャンパス（愛知県）
• [Presentation] Peroxide-reactivity of copper complexes supported by tridentate ligands having cyclic-diamine frameworks (2014)
  • Author(s): Tsukasa Abe, Takashi Ogura, Yuma Morimoto, Hideki Sugimoto, Nobutaka Fujieda, and Shinobu Itoh
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学 東山キャンパス（愛知県）
• [Presentation] ヘム鉄の電子密度の変化がデオキシミオグロビンのFe-His配位結合に及ぼす影響 (2014)
  • Author(s): 西村 龍, 柴田友和, 太 虎林, 石上 泉, 柳澤幸子, 小倉尚志, 根矢三郎, 鈴木秋弘, 山本泰彦
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学 東山キャンパス（愛知県）
• [Presentation] Resonance Raman Study on Cytochrome c Oxidase Reconstituted in the Phospholipids Vesicles (2013)
  • Author(s): Takashi Nomura, Sachiko Yanagisawa, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, and Takashi Ogura
  • Organizer: The 4th Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC-4)
  • Place of Presentation: Parry Sound, Canada
• [Presentation] Interaction Between Heme and Heme-Cu Binuclear center in Cytochrome c oxidase (2013)
  • Author(s): Miyuki Sakaguchi, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, and Takashi Ogura
  • Organizer: The 4th Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC-4)
  • Place of Presentation: Parry Sound, Canada
• [Presentation] Detection of tryptophan as bound substrate in indoleamine 2,3-dioxygenase (2013)
  • Author(s): Sachiko Yanagisawa, Masayuki Hara, Hiroshi Sugimoto, Yoshitsugu Shiro, and Takashi Ogura
  • Organizer: The 4th Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC-4)
  • Place of Presentation: Parry Sound, Canada
• [Presentation] Identification of functionally important conformational changes in cytochrome c oxidase (2013)
  • Author(s): Takashi Ogura
  • Organizer: The 4th Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC-4)（招待講演）
  • Place of Presentation: Parry Sound, Canada
  • Invited
• [Presentation] ドメインスワップしたシトクロムc多量体の形成および安定性に影響を及ぼす因子 (2013)
  • Author(s): 廣田 俊, Ari Dwi Nugraheni, 山城信裕, 安藤勇輝, 長尾 聡, Zhonghua Wang, 柳澤幸子, 小倉尚志
  • Organizer: 第40回生体分子科学討論会
  • Place of Presentation: 大阪大学吹田キャンパス（大阪府）
• [Presentation] 基質結合型インドールアミン2,3 ジオキシゲナーゼの基質の検出-紫外共鳴ラマン分光法 (2013)
  • Author(s): 柳澤幸子, 原 雅行, 杉本 宏, 城 宜嗣, 小倉尚志
  • Organizer: 第40回生体分子科学討論会
  • Place of Presentation: 大阪大学吹田キャンパス（大阪府）
• [Presentation] 休止酸化型チトクロムc酸化酵素の酸化還元部位の化学構造 (2013)
  • Author(s): 坂口美幸, 新澤-伊藤恭子, 吉川信也, 小倉尚志
  • Organizer: 感応性化学種が拓く新物質科学第２回公開シンポジウム
  • Place of Presentation: 九州大学医学部百年記念講堂（福岡県）
• [Presentation] 酵素反応中に見られる感応性化学種の振動分光法による補足と精密構造解析 (2013)
  • Author(s): 小倉尚志
  • Organizer: 感応性化学種が拓く新物質科学第２回公開シンポジウム
  • Place of Presentation: 九州大学医学部百年記念講堂（福岡県）
• [Presentation] チトクロムc酸化酵素の高効率プロトンポンプを可能にするタンパク質構造ダイナミクス: 振動分光法 (2013)
  • Author(s): 小倉尚志
  • Organizer: 第13回日本蛋白質科学会年会
  • Place of Presentation: とりぎん文化会館（鳥取県）
  • Invited
• [Presentation] Real time observation of water-channel closure controlled by CuB in bovine cytochrome c oxidase using a novel high-sensitivity infrared system (2013)
  • Author(s): Minoru Kubo, Satoru Nakashima, Satoru Yamaguchi, Takashi Ogura, Masao Mochizuki, Jiyoung Kang, Masaru Tateno, Kyoko Shinzawa-Itoh, Koji Kato, and Shinya Yoshikawa
  • Organizer: International Conference on BioInorganic Chemistry ICBIC-16
  • Place of Presentation: Grenoble, France
• [Presentation] Mechanism for ligand discrimination in myoglobin (2013)
  • Author(s): Yasuhiko Yamamoto, Ryu Nishimura, Tomokazu Shibata, Hulin Tai, Izumi Ishigami, Takashi Ogura, Satoshi Nagao, Takashi Matsuo, Shun Hirota, Kiyohiro Imai, Saburo Neya, and Akihiro Suzuki
  • Organizer: International Conference on BioInorganic Chemistry ICBIC-16
  • Place of Presentation: Grenoble, France
• [Presentation] High-resolution femtosecond crystallography at SACLA, a challenge of reducing the X-ray radiation damage on super molecular assemblies (2013)
  • Author(s): Hideo Ago, Kunio Hirata, Go Ueno, Hironori Murakami, Takehiko Tosha, Tamao Hisano, Minoru Kubo, Hiroshi Sugimoto, Yoshitsugu Shiro, Masaki Yamamoto, Eiki Yamashita, Jian-Ren Shen, Kyoko Shinzawa-Ito, Koji Kato, Miki Hatanaka, Shuhei Takemura, Naomine Yano, Takashi Ogura, Shinya Yoshikawa, and Tomitake Tsukihara
  • Organizer: 11th International Conference on Biology and Synchrotron Radiation (BSR)
  • Place of Presentation: Grand Elysee Hotel Hamburg, Germany
• [Presentation] 完全酸化型チトクロム c 酸化酵素の酸化還元活性金属中心とアザイドの相互作用の分光学的研究 (2013)
  • Author(s): Masahide Hikita, Akima Yamamoto, Tomoko Maeda, Kyoko Shinzawa-Itoh, Takashi Ogura, and Shinya Yoshikawa
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都国際会館（京都府）
• [Presentation] 発 表 標 題 チトクロムc 酸化酵素の酸素還元反応における赤外吸収測定を目的とした酸素肺フローシステムの開発 (2013)
  • Author(s): Tatsuhito Nishiguchi, Masahide Hikita, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Satoru Nakashima, and Takashi Ogura
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都国際会館（京都府）
• [Presentation] インドールアミン2,3 ジオキシゲナーゼの基質トリプトファンの検出-紫外共鳴ラマン分光法 (2013)
  • Author(s): achiko Yanagisawa, Masayuki Hara, Hiroshi Sugimoto, Yoshitsugu Shiro, and Takashi Oguara
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都国際会館（京都府）
• [Presentation] タンパク質中のトリプトファン残基の近紫外円二色性と紫外共鳴ラマンスペクトルの特性 (2013)
  • Author(s): higenori Nagatomo, Masako Nagai, Takashi Ogura, and Teizo Kitagawa
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都国際会館（京都府）
• [Presentation] Interaction Between Heme and Heme-Cu Binuclear Center in Cytochrome c Oxidase (2013)
  • Author(s): Miyuki Sakaguchi, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, and Takashi Ogura
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都国際会館（京都府）
• [Presentation] 生体高分子の機能制御におけるゆらぎの役割 (2013)
  • Author(s): 長友重紀
  • Organizer: つくばソフトマター研究会2013
  • Place of Presentation: 筑波大学（茨城県）
  • Invited
• [Presentation] ヘモグロビンの機能制御における四次構造変化、α鎖，β鎖の役割：共鳴ラマン分光による研究 (2013)
  • Author(s): 長友重紀
  • Organizer: 第26回生物無機化学夏季セミナー（生物無機化学研究会）
  • Place of Presentation: 茨城県、阿字ケ浦クラブ
  • Invited
• [Presentation] Thermodynamic Study of Cyclic Oligomers via Hydrogen Bonds in Organic Liquids (2013)
  • Author(s): higenori Nagatomo, Yasuhisa Yamamura, Kazuya Saito
  • Organizer: The Fifth International Symposium on the New Frontiers of Thermal Studies of Materials.
  • Place of Presentation: Yokohama Media and Communications Center（神奈川県）
• [Presentation] チトクロムc酸化酵素によるプロトンポンプ反応の研究:時間分解赤外および共鳴ラマン分光法 (2013)
  • Author(s): 小倉尚志
  • Organizer: 分子研研究会「生物物質科学の展望」
  • Place of Presentation: 分子研明大寺地区(愛知県)(招待講演)
• [Presentation] 生体配位化学における高解像度の虫眼鏡:振動分光法 (2013)
  • Author(s): 小倉尚志
  • Organizer: 分子研研究会「生体配位化学の最前線と展望」
  • Place of Presentation: 分子研明大寺地区(愛知県)(招待講演)
• [Presentation] ミトコンドリア呼吸鎖電子伝達複合体Iに含まれるFMNの共鳴ラマンスペクトル (2013)
  • Author(s): 引田理英、新澤伊藤恭子、森山昌和、小倉尚志、木平清人、吉川信也
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス(滋賀県)
• [Presentation] インドールアミン2, 3-ジオキシゲナーゼの共鳴ラマンスペクトル:基質結合による反応性の調節 (2013)
  • Author(s): 柳澤幸子、原 雅之、杉本 宏、城宜嗣、小倉尚志
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス(滋賀県
• [Presentation] カルボン酸含有二核化配位子(BPG2E)を用いたパーオキソ二核鉄(III)錯体のO-O結合の開裂と基質酸化活性 (2013)
  • Author(s): 平野智也、河原由佳、人見穣、小寺政人、小林義男、野村高志、小倉尚志
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス(滋賀県)
• [Presentation] Molecular Science of the Proteins in Organelles, Physiological Molecular Science of Membrane Proteins (2012)
  • Author(s): Takashi Ogura
  • Organizer: 分子高次系機能解明のための分子科学成果公開シンポジウム
  • Place of Presentation: 東京工業大学すずかけ台キャンパス(東京都)(招待講演)
• [Presentation] ヒト成人ヘモグロビンへのベザフィブラートの結合による酸素親和性低下に関連した三次構造変化の解明 (2012)
  • Author(s): 長友重紀,濱田洋実,吉川裕之,斎藤一弥
  • Organizer: 第39回生体分子科学討論会
  • Place of Presentation: 東北大学片平さくらホール(宮城県)
• [Presentation] トリプトファンの近紫外円二色性及び紫外共鳴ラマンに基づくヘモグロビンの四次構造変化の解明 (2012)
  • Author(s): 長井雅子,長友重紀,長井幸史,大久保賢一,今井清博,北川禎三
  • Organizer: 第39回生体分子科学討論会
  • Place of Presentation: 東北大学片平さくらホール(宮城県)
• [Presentation] 共鳴ラマン分光法によるインドールアミン2,3ジオキシゲナーゼの反応機構の研究 (2012)
  • Author(s): 柳澤幸子,原雅行,杉本宏,城宜嗣,小倉尚志
  • Organizer: 第39回生体分子科学討論会
  • Place of Presentation: 東北大学片平さくらホール(宮城県)
• [Presentation] Structural features in Functions of Heme Proteins Revealed by Resonance Raman Spectroscopy (2012)
  • Author(s): Teizo Kitagawa
  • Organizer: 国際プルフィリン・フタロシャニン学会
  • Place of Presentation: Jeju (Korea)(招待講演)
• [Presentation] Cooperative Dynamics of the Two Hemes in Cytochrome c Oxidase upon Ligand Binding Identified by Time-Resolved Resonance Raman Spectroscopy (2012)
  • Author(s): Takashi Ogura, Izumi Ishigami, Takeshi Nishigaki, Satoru Nakashima, Kyoko Shinzawa-Itoha, and Shinya Yoshikawa
  • Organizer: The 7th International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: Jeju (Korea)(招待講演)
• [Presentation] Dioxygenation reaction of indoleamine 2,3-dioxygenase as studied by resonance Raman spectroscopy (2012)
  • Author(s): Sachiko Yanagisawa, Masayuki Hara, Hiroshi Sugimoto, Yoshitsugu Shiro, and Takashi Ogura
  • Organizer: The 7th International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: Jeju (Korea)(招待講演)
• [Presentation] Proton Pump Mechanism of Cytochrome c Oxidase Elucidated by Highly Sensitive Time-Resolved IR Spectroscopy (2012)
  • Author(s): Satoru Nakashima, Minoru Kubo, Satoru Yamaguchi, Takashi Ogura, Masao Mochizukia, Kyoko Shinzawa-Itoh, and Shinya Yoshikawa
  • Organizer: The 7th International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: Jeju (Korea)(招待講演)
• [Presentation] Protein Dynamics of Cytochrome c Oxidase Studied by Time-Resolved Resonance Raman and Infrared Spectroscopy : Coupling between 0_2 Reduction and H^+-Pumping (2012)
  • Author(s): Takashi Ogura
  • Organizer: 国際ラマン分光学会
  • Place of Presentation: Bangalore (India)(招待講演)
• [Presentation] Effects of Heme Rotation around the Heme Normal by 90 °on Resonance Raman Spectra of Heme Proteins : Observation for Heme Oxygenase (2012)
  • Author(s): Sachiko Yanagisawa, Hiroshi Fujii, Saburo Neya, Takashi Ogura, and Teizo Kitagawa
  • Organizer: 国際ラマン分光学会
  • Place of Presentation: Bangalore (India)(招待講演)
• [Presentation] Essence in oxygen affinity regulation of human adult hemoglobin : Change of Fe-His bond length by quaternary or tertiary structure change (2012)
  • Author(s): Shigenori Nagatomo, Yasuhisa Yamamura, and Kazuya Saito
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Nagoya University (Aichi)
• [Presentation] Substitution effects of Gly for the proximal His of α subunits on the structure and oxygen binding function of human hemoglobin (2012)
  • Author(s): Yayoi Aki, Yukifumi Nagai, Kiyohiro Imai, Shigenori Nagatomo, Takashi Ogura, Teizo Kitagawa, and Masako Nagai
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Nagoya University (Aichi)
• [Presentation] An Intermediate Conformation of Cytochrome Oxidase in the Ligand-Free State Identified by Time-Resolved Resonance Raman Spectroscopy (2012)
  • Author(s): Izumi Ishigami, Takeshi Nishigaki, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Satoru Nakashima, and Takashi Ogura
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Nagoya University (Aichi)
• [Presentation] The assignment of the resonance Raman bands of heme a peripheral substituents in cytochrome c oxidase (2012)
  • Author(s): Miyuki Sakaguchi, Yukie Katayama, Hiroshi Fujii, Hideo Shimada, and Takashi Ogura
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Nagoya University (Aichi)
• [Presentation] Ultraviolet Resonance Raman Study on Indoleamine 2, 3-Dioxygenase (2012)
  • Author(s): Masayuki Hara, Sachiko Yanagisawa, Hiroshi Sugimoto, Yoshitsugu Shiro, and Takashi Ogura
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Nagoya University (Aichi)
• [Presentation] Effects of Heme Rotation around the Heme Normal by 90°on Resonance Raman Spectra of Heme Proteins; Observation for Heme Oxygenase (2012)
  • Author(s): Sachiko Yanagisawa, Hiroshi Fujii, Saburo Neya, Takashi Ogura, and Teizo Kitagawa
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Nagoya University (Aichi)
• [Presentation] Time-Resolved IR Analyses of Proteins Using a Novel Pulse Flow System Synchronized with Pump/Probe Lasers (2012)
  • Author(s): Minoru Kubo, Satoru Nakashima, Masao Mochizuki, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Takashi Ogura, and Teizo Kitagawa
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Nagoya University (Aichi)
• [Presentation] Structural Mechanism of Target Molecule Recognition by Gas Sensor Heme Proteins (2012)
  • Author(s): Teizo. Kitagawa
  • Organizer: アジア生物無機化学会
  • Place of Presentation: 香港(中国)(招待講演)
• [Presentation] 巨大タンパク質内での反応を観る:時間分解赤外および共鳴ラマン分光法 (2012)
  • Author(s): 小倉尚志
  • Organizer: 第1回分光イノベーションシンポジウム
  • Place of Presentation: 東京工業大学・百年記念館(東京都)(招待講演)
• [Presentation] Relation among Fe-His bond, fluctuations of helices and oxygen affinity of hemoglobin in T-state and R-state (2012)
  • Author(s): Shigenori Nagatomo, Yukiko Ozeki, Yasuhisa Yamamura, and Kazuya Saito
  • Organizer: Molecular Science of Fluctuations toward Biological Functions, 6th International Symposium
  • Place of Presentation: Kyoto University (Kyoto)
• [Presentation] ヒトヘモグロビンの酸素結合機能における近位ヒスチジンの役割 (2012)
  • Author(s): 丸山夏未,安芸弥生,長井幸史,今井清博,長友重紀,小倉尚志,北川禎三,長井雅子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡県)