Development of A General Single-Molecular Detection System Utilizing Nanomechanical DNA Origami Devices
| Project/Area Number |
24350088
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kansai University |
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Principal Investigator |
Kuzuya Akinori 関西大学, 化学生命工学部, 准教授 (00456154)
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| Co-Investigator(Kenkyū-buntansha) |
OHYA Yuichi 関西大学, 化学生命工学部, 教授 (10213886)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | DNAオリガミ / 単分子検出 / 生体分子 / DNAナノテクノロジー / 核酸化学 / 分子ロボティクス / 原子間力顕微鏡 / アガロースゲル電気泳動 / 分子計算 / 核酸 / ナノバイオ / 分子機械 / 分子認識 / 単分子機能デバイス / DNA / ナノ構造 / 酵素 / ナノ材料 / 金ナノ粒子 / 一分子解析 |
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| Outline of Final Research Achievements |
A single-molecular detection system utilizing our original nanomechanical DNA origami devices (DNA pliers) composed of two 170-nm long stick-like components connected to each other at a fulcrum has been applied to more practical use by improving the molecular design and nanomechanical properties. DNA pliers can now detect protons, theoretically smallest substance, as well as special binding event of bio-related molecules such as PNA strand invasion. Introduction of a tubular design enabled handy detection of structure change of DNA pliers with popularly available gel electrophoresis.
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Report
(5 results)
Research Products
(79 results)
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[Journal Article] A DNA aptamer recognising a malaria protein biomarker can function as part of a DNA origami assembly2016
Author(s)
Godonoga M., LinTY., Oshima A., Sumitomo K., Tang MS., Cheung YW., Kinghorn AB., Dirkzwager RM., Zhou C., Kuzuya A., Tanner JA. and Heddle JG.
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 21266-21266
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Smart Hydrogels Based on DNA Quadruplexes2015
Author(s)
A. Kuzuya, S. Tanaka, K. Fukushima, K. Wakabayashi, Y. Ohya
Organizer
The International Chemical Congress of Pacific Basin Societies 2015 (PACIFICHEM2015)
Place of Presentation
ハワイ州ホノルル（アメリカ）
Year and Date
2015-12-18
Related Report
Int'l Joint Research
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[Presentation] DNA Sudare: A NancrSized Wrapper2012
Author(s)
A. Kuzuya, S. Minamida, M. Kaino, M. Hashizume, Y. Ohya
Organizer
The 39th International Symposium on Nucleic Acids Chemistry
Place of Presentation
Nagoya University
Year and Date
2012-11-17
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