| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
Graphene oxide (GO) is widely used in biosensors, because GO can absorb a fluorophore-labeled DNA and efficiently quench the fluorescence. However, the adsorption process remains unclear yet.
In this study, we have studied the following topics. (1) Properties of various DNA structures in non-ideal conditions, as observed in the GO nanosurface: It was found that hydration state of DNA strands was critically important for structure and thermodynamic stability of DNAs under non-ideal conditions. (2) The adsorption mechanism of DNA onto GO nanosurface: We studied thermodynamically and kinetically the mechanism, and found that the binding affinity of DNA was independent of the DNA structure but kinetics was largely depending on the structure. (3) Development of sensing ligands and screening systems for DNA G-quadruplexes: We found ThT, a typical fluorescence probe for protein fibril, was able to bind specifically to DNA G-quadruplex with bright fluorescence signals.
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