Quantitative analysis of DNA on GO nanosurface and development of DNA binding ligand screening system
Project/Area Number |
24350089
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Chemistry related to living body
|
Research Institution | Konan University |
Principal Investigator |
MIYOSHI Daisuke 甲南大学, フロンティアサイエンス学部, 教授 (50388758)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
|
Keywords | グラフェン / DNA / 熱力学的解析 / 速度論的解析 / テロメア / 四重らせん構造 / リガンド / バイオセンサー / 相互作用 / 吸着 / 核酸 / 分子認識 / ナノバイオ |
Outline of Final Research Achievements |
Graphene oxide (GO) is widely used in biosensors, because GO can absorb a fluorophore-labeled DNA and efficiently quench the fluorescence. However, the adsorption process remains unclear yet. In this study, we have studied the following topics. (1) Properties of various DNA structures in non-ideal conditions, as observed in the GO nanosurface: It was found that hydration state of DNA strands was critically important for structure and thermodynamic stability of DNAs under non-ideal conditions. (2) The adsorption mechanism of DNA onto GO nanosurface: We studied thermodynamically and kinetically the mechanism, and found that the binding affinity of DNA was independent of the DNA structure but kinetics was largely depending on the structure. (3) Development of sensing ligands and screening systems for DNA G-quadruplexes: We found ThT, a typical fluorescence probe for protein fibril, was able to bind specifically to DNA G-quadruplex with bright fluorescence signals.
|
Report
(4 results)
Research Products
(84 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] THIOFLAVINE-T AS HUMAN TELOMERIC G-QUADRUPLEX PROBE2012
Author(s)
Daisuke Miyoshi, Valerie Gabelica, Ryuichi Maeda, Takeshi Fujimoto, Hidenobu Yaku, Ta-kashi Murashima, Naoki Sugimoto
Organizer
XX International Roundtable on Nucleosides, Nucleotides and Nucleic Acids
Place of Presentation
Montreal (Canada)
Year and Date
2012-08-06
Related Report
-
-
-
-
-
-
-