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Development of an ecological and economical urine sensing system for diabetic disease screening

Research Project

Project/Area Number 24360141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Electron device/Electronic equipment
Research InstitutionOsaka Institute of Technology

Principal Investigator

Mitsuaki Yano  大阪工業大学, 工学部, 教授 (40200563)

Co-Investigator(Kenkyū-buntansha) KOIKE Kazuto  大阪工業大学, 工学部, 准教授 (40351457)
SASA Shigehiko  大阪工業大学, 工学部, 教授 (50278561)
TSUTSUI Hiroshi  大阪工業大学, 工学部, 教授 (00351453)
OMATU Shigeru  大阪工業大学, 工学部, 教授 (30035662)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Keywords先端機能デバイス / 計測工学 / 電子デバイス・機器
Outline of Final Research Achievements

The target of this study is to develop ecological and economical urine sensing systems for diabetic disease screening. For this purpose, we developed a solution-gate field-effect transistor (SGFET) made of a Ta2O5/ZnO film on a glass substrate, and immobilized proton receptors, globulin aptamers, or glucose oxidase molecules on the gate electrode. These SGFET sensors were demonstrated to be able to detect the concentrations of protons, globulins, and β-D glucose in solution with enough sensitivity and detection range required for the screening. We also developed the MEMS technology to integrate these SGFETs into a healthcare chip, original ceramic gas sensors to check the characteristics of diabetic odor in urine or exhaled breath, and a prototype expert system of an electronic nose using a layered neural network.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (52 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (18 results) (of which Peer Reviewed: 17 results,  Acknowledgement Compliant: 11 results,  Open Access: 3 results) Presentation (32 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results) Remarks (2 results)

  • [Journal Article] Growth and crystallographic characterization of molecular beam epitaxial WO3 and MoO3/WO3 thin films on sapphire substrates2016

    • Author(s)
      M. Yano, K. Koike, M. Matsuo, T. Murayama, Y. Harada, K. Inaba
    • Journal Title

      Applied Surface Science

      Volume: 未定 Pages: 32-35

    • DOI

      10.1016/j.apsusc.2016.01.097

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A potentiometric glucose sensing by an enzyme-modified Ta2O5/ZnO/Zn0.6Mg0.4O solution-gate field effect transistor2015

    • Author(s)
      K. Iketani, K. Koike, Y. Hirofuji, T. Maemoto, S. Sasa, M. Yano
    • Journal Title

      IEEE Explore

      Volume: IMFEDK2015 Pages: 40-41

    • DOI

      10.1109/imfedk.2015.7158539

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characteristics of a Microbridge-type MEMS Gas Sensor for the Thermophysical Detection by a Steady-state Hot Wire Method2015

    • Author(s)
      矢野満明、藤井建太、村岡茂信、大松繁
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 135 Issue: 7 Pages: 263-268

    • DOI

      10.1541/ieejsmas.135.263

    • NAID

      130005086210

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characteristics of P-type Semiconducting MgCr<sub>2</sub>O<sub>4</sub>-TiO<sub>2</sub> Ceramics for Gas Sensing Devices2015

    • Author(s)
      矢野満明、平原陽介、寺田二郎、佐々誠彦、大松 繁
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 135 Issue: 8 Pages: 317-322

    • DOI

      10.1541/ieejsmas.135.317

    • NAID

      130005090448

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mixed odors classification by neural networks2015

    • Author(s)
      S. Omatu, M. Yano
    • Journal Title

      Intelligent Data Acquisition and Advanced Computing Systems: Technology and Applications

      Volume: 1 Pages: 171-176

    • DOI

      10.1109/idaacs.2015.7340723

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A potentiometric immunosensor based on a ZnO field-effect transistor2014

    • Author(s)
      K. Koike, K. Mukai, T. Onaka, T. Maemoto, S. Sasa, M. Yano
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 53 Issue: 5S1 Pages: 05FF04-05FF04

    • DOI

      10.7567/jjap.53.05ff04

    • NAID

      210000143833

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characteristics of a microbridge type MEMS sensor for the thermal conductivity measurement of gases by a steady state method2014

    • Author(s)
      K. Fujii, S. Muraoka, S. Omatu, M. Yano
    • Journal Title

      IEEE Explore

      Volume: なし Pages: 2-2

    • DOI

      10.1109/imfedk.2014.6867052

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Zinc Oxide Ion-Sensitive Field-Effect Transistors and Biosensors2014

    • Author(s)
      Mitsuaki Yano, Kazuto Koike, Kazuya Mukai, Takayuki Onaka, Yuichi Hirofuji, Kenichi Ogata, Sigeru Omatu, Toshihiko Maemoto, Shiegehiko Sasa
    • Journal Title

      Physica Status Solidi (a)

      Volume: A211 Issue: 9 Pages: 2098-2104

    • DOI

      10.1002/pssa.201300589

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characteristics of P-type (Zn<sub>1</sub><sub>-<i>x</i></sub>Mg<i><sub>x</sub></i>)Cr<sub>2</sub>O<sub>4</sub>-TiO<sub>2</sub> Ceramics for Gas-sensing Applications2014

    • Author(s)
      寺田二郎、平原陽介、大松 繁、佐々誠彦、矢野満明
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 134 Issue: 9 Pages: 308-314

    • DOI

      10.1541/ieejsmas.134.308

    • NAID

      130004684899

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] MgCr2O4-TiO2系P型半導体セラミックスのガス検出特性2014

    • Author(s)
      平原陽介,寺田二郎,大松 繁,佐々誠彦,矢野満明
    • Journal Title

      第31回センサ・マイクロマシンと応用システムシンポジウム講演論文集

      Volume: なし

    • NAID

      130005090448

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 熱線式マイクロブリッジMEMSセンサのガス検出特性2014

    • Author(s)
      藤井建多,村岡茂信,大松 繁,矢野満明
    • Journal Title

      第31回センサ・マイクロマシンと応用システムシンポジウム講演論文集

      Volume: なし

    • NAID

      130005086210

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characteristics of MoO3 films grown by molecular beam epitaxy2014

    • Author(s)
      K. Koike, R. Wada, S. Yagi, Y. Harada, S. Sasa, M. Yano
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 53 Issue: 5S1 Pages: 05FJ02-05FJ02

    • DOI

      10.7567/jjap.53.05fj02

    • NAID

      210000143849

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Smell classification using weakly responding data2014

    • Author(s)
      S. Omatu, M. Yano
    • Journal Title

      Proc. of 29th Annual ACM Symposium on Applied Computing

      Volume: 0001 Pages: 213-214

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blood plasma separator using micro pillars arranged like a labyrinth2013

    • Author(s)
      H. Tsutsui, H. Miyagawa, M. Yano
    • Journal Title

      Proc. of 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences

      Volume: 0001 Pages: 587-589

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rice-planted area extraction by RADARSAT data using lerning vector quantization algorithm2013

    • Author(s)
      S. Omatu, M. Yano
    • Journal Title

      Proc. of 7th International Conference on Advanced Engineering Computing and Applications in Sciences

      Volume: 0001 Pages: 19-23

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 酸化亜鉛薄膜の放射線耐性2012

    • Author(s)
      矢野、小池、佐々、権田、石神、久米,
    • Journal Title

      機能材料

      Volume: 32 (12)(依頼論文) Pages: 4-11

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 高性能酸化亜鉛系FETと酸化物デバイス用の広がり2012

    • Author(s)
      佐々, 矢野, 前元, 小池, 尾形
    • Journal Title

      電子情報通信学会誌

      Volume: 95 (4) Pages: 289-293

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mixed Odor Classification for QCM Sensor Data by Neural Network2012

    • Author(s)
      S. Omatu, H. Araki, T. Fujinak, M. Yano, M. Yoshio, H. Nakazum, I. Tanahasi
    • Journal Title

      Advances in Distributed Computing and Artificial Intelligence Journal

      Volume: 2 Pages: 35-42

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Glucose sensing by an enzyme-modified ZnO-based FET2016

    • Author(s)
      M. Yano, Y. Mori, S. Sasa, Y. Hirofuji, K. Koike
    • Organizer
      30th EUROSENSORS
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2016-09-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Smells Classification for Human Breath Using a Layered Neural Network2016

    • Author(s)
      S. Omatu, M. Yano
    • Organizer
      13th International Conference on Distributed Computing and Artificial Intelligence
    • Place of Presentation
      Seville, Spain
    • Year and Date
      2016-06-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロ血球分ルフィルタに関する研究2016

    • Author(s)
      加藤拓真、筒井博司
    • Organizer
      第63回応用物理学会春期学術講演会
    • Place of Presentation
      東京工業大学、東京都
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] ゾルゲル成膜したZnO薄膜の乾燥温度依存性とTFTへの応用2015

    • Author(s)
      川上祐貴、尾形健一、小山政俊、前元利彦、佐々誠彦
    • Organizer
      日本材料学会平成27年度半導体エレクトロニクス部門第2回研究会
    • Place of Presentation
      京都大学桂キャンパス、京都市
    • Year and Date
      2015-11-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mixed Odors Classification by Neural Networks2015

    • Author(s)
      S. Omatu, M. Yano
    • Organizer
      8th IEEE International Conference on Intelligent Data Acquisition and Advanced Computing Systems: Technology and Applications
    • Place of Presentation
      Warsow, Poland
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Slipping Characteristics of Multichannel Flexible Tactile Sensor applicable for a Robot Finger2015

    • Author(s)
      H. Tsutsui, H. Kobayashi
    • Organizer
      37th Annual Int. Conf. of the IEEE Engineering in Medicine and Biology Society
    • Place of Presentation
      Milan, Itary
    • Year and Date
      2015-08-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A potentiometric glucose sensor based on an enzyme-modified Ta2O5/ZnO/ZnMgO solution-gate field-effect transistor2015

    • Author(s)
      K. Iketani, K. Koike, Y. Hirofuji, T. Maemoto, S. Sasa, M. Yano
    • Organizer
      13th Int. Mtg. for Future of Electron Devices, Kansai
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2015-06-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロ構造を利用した白血球分離の試み2015

    • Author(s)
      藤内陽介、加藤拓真、筒井博司
    • Organizer
      第54回日本生体医工学会大会
    • Place of Presentation
      名古屋国際会議場、名古屋市
    • Year and Date
      2015-05-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] ゾルゲル成膜した酸化亜鉛薄膜の乾燥温度依存性とTFT特性2015

    • Author(s)
      川上祐貴,大東隆文,尾形健一,前元利彦,佐々誠彦
    • Organizer
      第62回応用物理学関係連合講演会
    • Place of Presentation
      東海大学
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 酸化亜鉛系電界効果トランジスターを用いたグルコースセンサーの作製と評価2015

    • Author(s)
      池谷謙,小池一歩,広藤裕一,前元利彦,佐々誠彦,矢野満明
    • Organizer
      第62回応用物理学関係連合講演会
    • Place of Presentation
      東海大学
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] MgCr2O4-TiO2系P型半導体セラミックスのガス検出特性2014

    • Author(s)
      平原陽介,寺田二郎,大松 繁,佐々誠彦,矢野満明
    • Organizer
      日本材料学会半導体エレクトロニクス部門,平成26年度第2回研究会
    • Place of Presentation
      神戸大学
    • Year and Date
      2014-11-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] ZnO Devices: Current and Future2014

    • Author(s)
      S. Sasa, M. Yano, K. Koike, T. Maemoto, K. Ogata
    • Organizer
      Asia Pacific Microwave Conference Workshop 2014
    • Place of Presentation
      Sendai
    • Year and Date
      2014-11-04 – 2014-11-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] MgCr2O4-TiO2系P型半導体セラミックスのガス検出特性2014

    • Author(s)
      平原陽介,寺田二郎,大松 繁,佐々誠彦,矢野満明
    • Organizer
      第31回センサ・マイクロマシンと応用システムシンポジウム
    • Place of Presentation
      松江
    • Year and Date
      2014-10-20 – 2014-10-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 熱線式マイクロブリッジMEMSセンサのガス検出特性2014

    • Author(s)
      藤井建多,村岡茂信,大松 繁,矢野満明
    • Organizer
      第31回センサ・マイクロマシンと応用システムシンポジウム
    • Place of Presentation
      松江
    • Year and Date
      2014-10-20 – 2014-10-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 骨格筋の培養とバイオアクチュエータへの応用2014

    • Author(s)
      宮本政典, 鳩間翔平, 高木空, 藤里俊哉, 筒井博司
    • Organizer
      第30回化学とマイクロナノシステム学会研究会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-10-02 – 2014-10-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] マイクロ構造を利用した白血球分離の試み2014

    • Author(s)
      藤内陽介,筒井博司
    • Organizer
      第30回化学とマイクロナノシステム学会研究会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-10-02 – 2014-10-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] ZnO溶液ゲート電界効果トランジスタを用いたバイオセンサの開発2014

    • Author(s)
      大仲崇之,池谷謙,小池一歩,広藤裕一,前元利彦,佐々誠彦,矢野満明
    • Organizer
      第75回応用物理学会秋季学術講演会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-09-17 – 2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of Multichannel Soft Tactile Sensors having Fingerprint Structure2014

    • Author(s)
      H. Tsutsui, Y. Murashima, N. Honma, K. Kobayashi
    • Organizer
      The 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
    • Place of Presentation
      Shicago, USA
    • Year and Date
      2014-08-26 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Characteristics of a microbridge type MEMS sensor for the thermal conductivity measurement of gases by a steady state method2014

    • Author(s)
      K.Fujii, S.Muraoka, S.Omatu, M.Yano
    • Organizer
      2014 International Meeting for Future of Electron Devices, Kansai
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-06-19 – 2014-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新対向ターゲット式スパッタ法によるTa205/Zn0 : Inヘテロ構造膜の作製と高感度pHセンサーへの応用2013

    • Author(s)
      向井、大仲、小池、前元、佐々、矢野、門倉、中光
    • Organizer
      第60回応用物理学会春季学術講演会
    • Place of Presentation
      神奈川工科大学、神奈川県
    • Year and Date
      2013-03-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] 新対向ターゲット式スパッタ法による単結晶ZnO薄膜のエピタキシャル成長2012

    • Author(s)
      田辺、向井、小池、前元、佐々、矢野、門倉、中光
    • Organizer
      第73回応用物理学会秋季学術講演会
    • Place of Presentation
      愛媛大学、愛媛県
    • Year and Date
      2012-09-13
    • Related Report
      2012 Annual Research Report
  • [Presentation] シリコン基板上に作製したマイクロブリッジ型熱細線によるガスの流速と混合比の測定

    • Author(s)
      藤井 建多、村岡 茂信、矢野 満明
    • Organizer
      2014年電子情報通信学会総合大会
    • Place of Presentation
      新潟大学(新潟市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ZnO溶液ゲート電界効果トランジスタを用いた免疫センサの作製と評価

    • Author(s)
      大仲崇之、池谷 謙、広藤裕一、小池一歩、前元利彦、佐々誠彦、矢野満明
    • Organizer
      第61回応用物理学会春季学術講演会
    • Place of Presentation
      青山学院大学(相模原市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] サファイア基板上にMBE成長したMoO3薄膜の結晶構造評価

    • Author(s)
      八木信治、松尾昌幸、小池一歩、原田義之、佐々誠彦、矢野満明、稲葉勝彦
    • Organizer
      第61回応用物理学会春季学術講演会
    • Place of Presentation
      青山学院大学(相模原市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Smell classification using weakly responding data

    • Author(s)
      S. Omatu, M. Yano
    • Organizer
      29th Annual ACM Symposium on Applied Computing
    • Place of Presentation
      Gyeongju, Korea
    • Related Report
      2013 Annual Research Report
  • [Presentation] Zinc oxide ion-sensitive field-effect transistors and biosensors

    • Author(s)
      M. Yano, K. Koike, K. Mukai, T. Onaka, Y. Hirofuji, K. Ogata, S. Omatu, T. Maemoto, S. Sasa
    • Organizer
      European Materials Research Society 2013 Fall Meeting
    • Place of Presentation
      Warsaw, Poland
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Blood plasma separator using micro pillars arranged like a labyrinth

    • Author(s)
      H. Tsutsui, H. Miyagawa, M. Yano
    • Organizer
      17th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Freiburg, Germany
    • Related Report
      2013 Annual Research Report
  • [Presentation] Potentiometric immunosensors based on a ZnO field-effect transistor

    • Author(s)
      K. Mukai, T. Onaka, K. Koike, T. Maemoto, S. Sasa, M. Yano
    • Organizer
      2013 JSAP-MRS Joint Symposia
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] Growth of a sputtered Ta2O5/ZnO film and its application to an ion-sensitive field-effect transistor

    • Author(s)
      K. Mukai, T. Onaka, K. Koike, T. Maemoto, S. Sasa, M. Yano
    • Organizer
      IEEE 2013 Future of Electron Devices, Kansai
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] Fabrication of zinc oxide thin film transistors using a facing-target sputtering method

    • Author(s)
      Y. Okada, R. Morita, K. Ogata, K. Koike, T. Maemoto, M. Yano
    • Organizer
      IEEE 2013 Future of Electron Devices, Kansai
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] Rice-planted area extraction by RADARSAT data using lerning vector quantization algorithm

    • Author(s)
      S. Omatu, M. Yano
    • Organizer
      7th International Conference on Advanced Engineering Computing and Applications in Sciences
    • Place of Presentation
      Port, Portugal
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characteristics of MoO3 films grown by molecular beam epitaxy

    • Author(s)
      R. Wada, S. Yagi, K. Koike, Y. Harada, S. Sasa, M. Yano
    • Organizer
      2013 JSAP-MRS Joint Symposia
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2013 Annual Research Report
  • [Remarks] 大阪工業大学ナノ材料マイクロデバイス研究センター「プロジェクトの研究成果一覧」

    • URL

      http://www.oit.ac.jp/japanese/nanotech/project/archive.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 大阪工業大学ナノ材料マイクロデバイス研究センター「プロジェクトの研究成果一覧」に記載

    • Related Report
      2014 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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