Dynamics and Ecological Risk of Lake Biwa Natural Organic Matter by Stable Isotope Labelling and Omics Analysis

• Project/Area Number: 24360216
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: Kyoto University
• Principal Investigator: SHIMIZU Yoshihisa 京都大学, 工学(系)研究科(研究院), 教授 (20226260)
• Co-Investigator(Kenkyū-buntansha): KUSAKABE Taketoshi 京都大学, 工学研究科, 特定研究員 (40462585)
• Co-Investigator(Renkei-kenkyūsha): MATSUDA Tomonari 京都大学, 工学研究科, 准教授 (50273488) ; HAYAKAWA Kazuhide 滋賀県琵琶湖環境科学研究センター, 総合解析部門, 専門研究員 (80291178) ; OKAMOTO Takahiro 滋賀県琵琶湖環境科学研究センター, 環境監視部門, 専門員 (70508473)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2012: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 琵琶湖 / 天然有機物 / 溶存有機物 / 安定同位体標識 / 環境動態 / 生態影響評価 / 湖沼環境

Outline of Final Research Achievements

Annual amount of organic carbon eluted from sediment of Lake Biwa was estimated at 510 tC from elution tests with undisturbed sediment cores. It is suggested that hypoxia (oxygen depletion) near the bottom of Lake Biwa increases organic loads from the sediment to water column. Molecular markers of refractory organic matter derived from Microcystis aeruginosa were demonstrated by stable isotope labelling and MS- and NMR- based omics analysis. Photosynthesis inhibition of natural dissolved organic matter was significantly detected by a ten-fold concentration.

Research Products

• [Journal Article] 琵琶湖北湖表層における天然有機物(NOM)の分解特性 (2012)
  • Author(s): 日下部武敏, 秋田泰典, 大谷壮介, 岡本高弘, 早川和秀, 清水芳久
  • Journal Title: 環境システム計測制御学会誌 Volume: 17 Pages: 102-111
  • NAID: 10031117663
  • Peer Reviewed
• [Presentation] Degradation of Microcystis aeruginosa by Pseudomonas fluorescens, and Production of Refractory Organic Matter in Lake (2015)
  • Author(s): S. Shen, T. Kusakabe, Y. Shimizu
  • Organizer: The 6th IWA-ASPIRE Conference & Exhibition
  • Place of Presentation: Beijing (China)
  • Year and Date: 2015-09-20 – 2015-09-24
• [Presentation] Characterization of Dissolved Organic Matter in the Northern Lake Biwa by Excitation-Emission Matrix Fluorescence Spectroscopy with Parallel Factor Analysis (2014)
  • Author(s): T. Kusakabe, S. Yamagata, N. Wada, Y. Shimizu
  • Organizer: Asian Core Program Comprehensive Symposium IV
  • Place of Presentation: Johor (Malaysia)
  • Year and Date: 2014-12-03 – 2014-12-04
• [Presentation] Evaluation of Algal Photosynthesis Inhibition Activity for Natural Organic Matter (2014)
  • Author(s): K. Misaki, Y. Morita, T. Kusakabe, Y. Shimizu
  • Organizer: Asian Core Program Comprehensive Symposium IV
  • Place of Presentation: Johor (Malaysia)
  • Year and Date: 2014-12-03 – 2014-12-04
• [Presentation] Biodegradability of Natural Organic Matter in the Northern Lake Biwa by Long-term Test Method (2014)
  • Author(s): T. Kusakabe, T. Okamoto, K. Hayakawa, Y. Shimizu
  • Organizer: The 9th IWA World Water Congress & Exhibition
  • Place of Presentation: Lisbon (Portugal)
  • Year and Date: 2014-09-21 – 2014-09-26
• [Presentation] DNA損傷検出系における天然有機物（NOM）の活性評価 (2014)
  • Author(s): 三﨑健太郎、松田俊、松田知成、日下部武敏、清水芳久
  • Organizer: 第23回環境化学討論会
  • Place of Presentation: 京都大学百周年時計台記念館（京都府・京都市）
  • Year and Date: 2014-05-14 – 2014-05-16
• [Presentation] 琵琶湖水中のフルボ酸が藻類と甲殻類へ与える影響について (2014)
  • Author(s): 早川和秀、廣瀬佳則、古田世子、一瀬諭、岡本高弘、田中仁志、三崎健太郎、日下部武敏、清水芳久、藤嶽暢英
  • Organizer: 第48回日本水環境学会年会
  • Place of Presentation: 東北大学川内北キャンパス
• [Presentation] 琵琶湖水の藻類光合成阻害活性評価 (2014)
  • Author(s): 三崎健太郎、盛田悠平、日下部武敏、清水芳久
  • Organizer: 第48回日本水環境学会年会
  • Place of Presentation: 東北大学川内北キャンパス
• [Presentation] 下水二次処理水中溶存有機物の藻類光合成阻害試験による生態影響評価 (2013)
  • Author(s): 盛田悠平、三崎健太郎、日下部武敏、清水芳久
  • Organizer: 京都大学環境衛生工学研究会第３５回シンポジウム
  • Place of Presentation: 京都大学百周年時計台記念館
• [Presentation] 琵琶湖水中のフルボ酸の生物影響評価手法について (2013)
  • Author(s): 早川和秀、岡本高弘、一瀬諭、古田世子、田中仁志、三崎健太郎、日下部武敏、清水芳久、藤嶽暢英
  • Organizer: 日本陸水学会第78回大会（大津大会）
  • Place of Presentation: 龍谷大学瀬田キャンパス
• [Presentation] 琶湖水中のフルボ酸の生物影響評価について (2013)
  • Author(s): 早川和秀、岡本高弘、一瀬諭、古田世子、田中仁志、三崎健太郎、日下部武敏、清水芳久、藤嶽暢英
  • Organizer: 日本腐植物質学会第29回講演会
  • Place of Presentation: 佐賀大学本庄キャンパス
• [Presentation] 琵琶湖北湖表層における天然有機物(NOM)の分解特性 (2012)
  • Author(s): 日下部武敏, 秋田泰典, 大谷壮介, 岡本高弘, 早川和秀, 清水芳久
  • Organizer: 環境システム計測制御学会第24回研究発表会
  • Place of Presentation: 横浜市開港記念会館(神奈川県)
  • Year and Date: 2012-10-26
• [Presentation] Seasonal Variation in Biodegradability of Natural Organic Matter (NOM) in the Northern Lake Biwa (2012)
  • Author(s): Kusakabe, T., Akita, Y., Otani, S., Nishida, M., Okamoto, T., Hayakawa, K., Minami, M., Okumura, Y., Shimizu, Y.
  • Organizer: IWA World Water Congress & Exhibition, Busan, Korea
  • Place of Presentation: BEXCO(韓国)
  • Year and Date: 2012-09-17