| Project/Area Number |
24360336
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
UEDA Hiroshi 東京工業大学, 資源化学研究所, 教授 (60232758)
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| Co-Investigator(Kenkyū-buntansha) |
DONG Jinhua 東京工業大学, 資源化学研究所, 助教 (80527838)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUYAMA Yuki (OHMURO Yuki) 神戸大学, 大学院工学研究科, 特命助教 (30571088)
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| Research Collaborator |
ABE Ryoji ウシオ電機, 研究員
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
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| Keywords | antibody / fluorescence quenching / immunoassay / cell-free translation / biochip / protein engineering / chemical biology / bioimaging / バイオテクノロジー / バイオセンサー / イメージング / 抗体工学 / 細胞分化 / 免疫測定 / 蛍光エネルギー移動 / 蛍光消光 / バイオイメージング / バイオチップ / 臨床診断 |
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| Outline of Final Research Achievements |
We aimed at the elucidation of basic principle of Quenchbody (Q-body) and its application to various fields. Q-body is a site-specifically fluorescence-labeled recombinant antibody fragment, which was recently discovered by ourselves. It can detect various antigens with high sensitivity and rapidity in situ.
We succeeded in synthesizing Fab-type Q-bodies not only using cell-free translation system but also using cell-based system, and found novel suitable fluorescent dyes and novel modification sites on antibody. We also found a way to make genetically encoded Q-bodies using GFP variants, succeeded in a bead-based assay and imaging of osteocalcin peptide secreted from the cells, and in creating a similar biosensor protein based on the other ligand binders called Q'-body.
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