| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
|
|---|
| Outline of Final Research Achievements |
To realize regenerative medicine, a technology to efficiently prepare a large number of differentiated cells of interest at low cost is necessary. However, differentiation-inducing factors such as cytokines are generally expensive. In this study, we designed chimeric receptors, in which the extracellular domain of cytokine receptors is replaced by single-chain antibody, which enables signaling in response to a cheap antigen instead of expensive cytokines. We aimed to develop a system to keep and expand stem cells in an undifferentiated state, and to differentiate them into specific lineages by expressing the cheap antigen-responsive chimeric receptors on the cell surface.
|
