Development of technologies for maintaining undifferentiated state and inducing growth and differentiation of human iPS cell by using chimeric receptor
| Project/Area Number |
24360337
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAGAMUNE Teruyuki 東京大学, 工学(系)研究科(研究院), 教授 (20124373)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAHARA Masahiro 東京大学, 大学院工学系研究科, 講師 (50345097)
YAMAGUCHI Satoshi 東京大学, 先端科学技術研究センター, 講師 (80398106)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | サイトカイン受容体 / キメラ蛋白質 / シグナル伝達 / 未分化維持技術 / 増殖・分化技術 / iPS細胞 / キメラ受容体 |
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| Outline of Final Research Achievements |
To realize regenerative medicine, a technology to efficiently prepare a large number of differentiated cells of interest at low cost is necessary. However, differentiation-inducing factors such as cytokines are generally expensive. In this study, we designed chimeric receptors, in which the extracellular domain of cytokine receptors is replaced by single-chain antibody, which enables signaling in response to a cheap antigen instead of expensive cytokines. We aimed to develop a system to keep and expand stem cells in an undifferentiated state, and to differentiate them into specific lineages by expressing the cheap antigen-responsive chimeric receptors on the cell surface.
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Report
(4 results)
Research Products
(47 results)
