| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Outline of Final Research Achievements |
B cells harboring aimed specific antigen-receptors were captured by antigen-expressing myeloma cells and selectively fused by electrical pulses to generate hybridoma cells secreting stereospecific monoclonal antibodies. By using this method, hybridoma cells can be fabricated to produce the stereospecific structure of the specific receptors on the cell membrane. Cell manipulation technique based on the dielectrophoresis was utilized to introduce the cells to the microwell array electrode and form single cell pairs with different types of cells rapidly and simply. Thus, the use of the present dielectrophoretic manipulation allows large numbers of cell pairs (over 100,000 pairs) to be produced within only 1 min.
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