Development of human iPS cell culture system using stirred tank bioreactors
| Project/Area Number |
24360340
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
SAKAI SHINJI 大阪大学, 基礎工学研究科, 准教授 (20359938)
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| Co-Investigator(Kenkyū-buntansha) |
TAYA Masahito 大阪大学, 大学院基礎工学研究科, 教授 (60144127)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
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| Keywords | iPS細胞 / 再生医療 / マイクロカプセル / 大量培養 / 組織工学 / 撹拌培養 / アルギン酸 / ヒドロゲル |
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| Outline of Final Research Achievements |
For realizing the regenerative medicine using human iPS cells, establishment of the technology of culturing the cells in huge amount is essential. In this study, we aimed to develop the microcapsules suitable for culturing iPS cells in stirred tank bioreactors. The microcapsule membrane acts as physical barriers for protecting the cells from shear forces given from the flow of surrounding solution. In addition, it is expected that the size of the cluster of the cells grown in the microcapsules can be defined by the microcapsule size. The method which we developed in this study realizes the microcapsule production with easier and shorter operation time compared with previous ones. The strength of the microcapsules prepared by the developed method was sufficient enough for incubating them in an air-lift type stirred tank bioreactor. In addition, enclosed human iPS cells grew in the microcapsules with keeping their undifferentiated state.
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Report
(4 results)
Research Products
(24 results)
