| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
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| Outline of Final Research Achievements |
For realizing the regenerative medicine using human iPS cells, establishment of the technology of culturing the cells in huge amount is essential. In this study, we aimed to develop the microcapsules suitable for culturing iPS cells in stirred tank bioreactors. The microcapsule membrane acts as physical barriers for protecting the cells from shear forces given from the flow of surrounding solution. In addition, it is expected that the size of the cluster of the cells grown in the microcapsules can be defined by the microcapsule size. The method which we developed in this study realizes the microcapsule production with easier and shorter operation time compared with previous ones. The strength of the microcapsules prepared by the developed method was sufficient enough for incubating them in an air-lift type stirred tank bioreactor. In addition, enclosed human iPS cells grew in the microcapsules with keeping their undifferentiated state.
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