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In vitro assay system for evaluating curative effect of myoblast sheet transplantation

Research Project

Project/Area Number 24360341
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionOsaka University

Principal Investigator

KINO-OKA Masahiro  大阪大学, 工学(系)研究科(研究院), 教授 (40234314)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2012: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Keywords細胞シート / 筋芽細胞 / 血管新生 / 細胞アッセイ / 流動性 / 再生医療 / 培養組織 / 創薬スクリーニング / シミュレーション / 骨格筋筋芽細胞 / 間葉系幹細胞 / ガン細胞 / 薬剤スクリーニング / シミュレータ
Outline of Final Research Achievements

Autologous transplantation of myoblast sheet has attracted attention as a new technique for curing myocardial infarction. Myoblast sheet has the ability to secret cytokines which improve heart function via the facilitation of angiogenesis on affected part. To understand in vivo phenomena and evaluate curative effect after transplantation such as cytokine secretion from transplant and its angiogenesis capability, a multi-layered cell sheet of human skeletal muscle myoblasts (HSMMs) was constructed by originally equipped gelatin stamp system. In the HSMM sheet, active cellular migration occurred in the horizontal and vertical directions anywhere, revealing the sheet fluidity. Dynamic behavior including collective migration and spatial habitation of heterogeneous cells, such as human skeletal muscle fibroblasts (HSMFs) and human umbilical vein endothelial cells (HUVECs), in the fluidic scaffold was clarified.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (34 results)

All 2015 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results) Presentation (27 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Spatial habitation of heterogeneous cell population in a multi-layered myoblast sheet due to the differences in their behaviors of migration and cell-cell connection2014

    • Author(s)
      Eiji Nagamori, Masashi Oda, Tadashi Nakamura, Tatsuya Shimizu, Teruo Okano and Masahiro Kino-oka
    • Journal Title

      Current Nanoscience

      Volume: 10 Issue: 2 Pages: 173-178

    • DOI

      10.2174/1573413709666131128235413

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] In vitro models for angiogenesis research: a review2014

    • Author(s)
      Trung Xuan Ngo, Eiji Nagamori, Tatsuya Shimizu, Teruo Okano, Masahito Taya and Masahiro Kino-oka
    • Journal Title

      International Journal of Tissue Regeneration

      Volume: 5 Pages: 37-45

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Endothelial cell behavior inside myoblast sheets with different thickness2013

    • Author(s)
      Trung Xuan Ngo, Eiji Nagamori, Tetsutaro Kikuchi, Tatsuya Shimizu, Teruo Okano, Masahito Taya and Masahiro Kino-oka
    • Journal Title

      Biotechnology letters

      Volume: 35 Issue: 7 Pages: 1001-1008

    • DOI

      10.1007/s10529-013-1174-x

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Network formation througn active migration of human vascular endothelial cells in a mult ilayered skeletal myoblast sheet2013

    • Author(s)
      Eiji Nagamori, Trung xuan Ngo, Yasunori Takezawa, Atsuhiro Saito,
    • Journal Title

      Biomaterials

      Volume: 34 Pages: 662-668

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Endothelial cell behavior inside Myoblast sheets with different thickness2013

    • Author(s)
      Trung xuan Ngo, Eij I Nagamori, Tetsutaro Kikuchi Tatsuya Shimizu,
    • Journal Title

      Biotechnology letters

      Volume: (in press)

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] コンピュータシミュレーションにおける未分化維持培養の時空間的表現と未分化状態からの逸脱2015

    • Author(s)
      房 宗平,Xuan Trung Ngo, 紀ノ岡 正博
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜, 横浜市
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 横紋筋肉腫由来RD細胞の筋芽細胞シートへの混入が内皮細胞ネットワーク形成に与える影響2015

    • Author(s)
      李 梦露,長森 英二,紀ノ岡 正博
    • Organizer
      化学工学会第80年会
    • Place of Presentation
      芝浦工業大学 豊洲キャンパス, 東京都江東区
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 移植用筋芽細胞群シート形成時に存在する変数がサイトカイン生成能に与える影響2015

    • Author(s)
      長森 英二,岩田 利彦,紀ノ岡 正博
    • Organizer
      化学工学会第80年会
    • Place of Presentation
      芝浦工業大学 豊洲キャンパス, 東京都江東区
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Kinetic model for expansion culture of iPS cells by considering the deviation from undifferentiated state on feeder2015

    • Author(s)
      Trung Xuan Ngo,Shuhei Fusa and Masahiro Kino-oka
    • Organizer
      化学工学会第80年会
    • Place of Presentation
      芝浦工業大学 豊洲キャンパス, 東京都江東区
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Kinetic model for expansion culture of iPS cells by considering the deviation from undifferentiated state on feeder layers2014

    • Author(s)
      Shuhei Fusa, Trung Xuan Ngo and Masahiro Kino-oka
    • Organizer
      The Termis-Americas 2014 Annual Conference & Exposition
    • Place of Presentation
      Washington,D.C., USA
    • Year and Date
      2014-12-13 – 2014-12-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Kinetic model for expansion culture of iPS cells by considering the deviation from undifferentiated state on feeder layers2014

    • Author(s)
      Trung Xuan Ngo, Shuhei Fusa and Masahiro Kino-oka
    • Organizer
      The 27th Annual and International Meeting of the Japanese Association for Animal Cell Technology
    • Place of Presentation
      Kitakyushu, Japan
    • Year and Date
      2014-11-11 – 2014-11-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 積層筋芽細胞シート内の間葉系幹細胞が血管内皮細胞挙動に与える影響2014

    • Author(s)
      中村 匡,長森 英二,紀ノ岡 正博
    • Organizer
      化学工学会第46回秋季大会
    • Place of Presentation
      九州大学 伊都キャンパス, 福岡市
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト骨格筋筋芽細胞群における異種細胞間接触がサイトカイン生成能に与える影響2014

    • Author(s)
      長森 英二, 岩田 利彦, 紀ノ岡 正博
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター, 札幌市
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] TGF-β1 添加が積層筋芽細胞シート内での血管内皮細胞挙動に与える影響2014

    • Author(s)
      村上 皓紀, 中村 匡, 長森 英二, 紀ノ岡 正博
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター, 札幌市
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Endothelial cell behavior inside myoblast sheets with different thickness2014

    • Author(s)
      Trung Xuan Ngo, Eiji Nagamori and Masahiro Kino-oka
    • Organizer
      Termis 2014EU Chapter Meeting
    • Place of Presentation
      Genova, Italy
    • Year and Date
      2014-06-10 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Understanding dynamic cell migration inside multilayered cell sheet using a kinetic model2014

    • Author(s)
      Kyoko Kishi, Trung Xuan Ngo and Masahiro Kino-oka
    • Organizer
      Termis 2014EU Chapter Meeting
    • Place of Presentation
      Genova, Italy
    • Year and Date
      2014-06-10 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 積層筋芽細胞シートにおける間葉系幹細胞の棲分け挙動の解析2014

    • Author(s)
      中村 匡,長森 英二,紀ノ岡 正博
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 積層筋芽細胞シート内に混在する異種細胞の棲分け挙動の解析2013

    • Author(s)
      中村匡
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2013-03-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] 積層細胞シートの厚みがシート内の血管内皮ネットワーク形成に与える影響2013

    • Author(s)
      長森英二
    • Organizer
      化学工学会第78年会
    • Place of Presentation
      大阪大学豊中キャンパス(大阪府)
    • Year and Date
      2013-03-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト細胞培養と細胞シートを用いた組織類似構造形成法2013

    • Author(s)
      紀ノ岡正博
    • Organizer
      精密工学会超精密加工専門委員会第65回研究会『ナノバイオエンジニアリングが拓く次世代基盤技術』
    • Place of Presentation
      メルパルク大阪(大阪府)(招待講演)
    • Year and Date
      2013-01-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] 虚血性心疾患治療に用いるヒト筋芽細胞シートの品質管理法2013

    • Author(s)
      長森英二,紀ノ岡正博
    • Organizer
      日本動物細胞工学会2013年度大会
    • Place of Presentation
      ホテルフジタ福井(福井県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] bFGF添加による細胞シート内における内皮ネットワーク形成の結合安定化と伸長2013

    • Author(s)
      長森 英二, 大澤 堯輝, 紀ノ岡 正博
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of Automated Cell Processing System for Clinical Application2013

    • Author(s)
      Masahiro Kino-oka
    • Organizer
      2013 AIChE Annual Meeting
    • Place of Presentation
      Hilton San Francisco Union Square (San Francisco, USA)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Tumor Angiogenesis Model Using Cell Sheet Technology2013

    • Author(s)
      Menglu Li, Eiji Nagamori and Masahiro Kino-oka
    • Organizer
      8th CELL BASED ASSAY & SCREENING TECHNOLOGIES
    • Place of Presentation
      Sheraton Fishermans Wharf (San Francisco, USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Network formation through active migration of human vascular endothellal cells in a mult ilayered skeletal myoblast sheet2012

    • Author(s)
      Trung Xuan Ngo
    • Organizer
      The 25 th Annual aM International Meeting of tne Japanese Association for Animal Cell Technology
    • Place of Presentation
      Nagoya Congress Center (Aichi)
    • Year and Date
      2012-11-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] Spatial habitation of umbilical vein endothelial or fibroblast cells in multilayered myoblast cell sheet2012

    • Author(s)
      Eiji Nagamori
    • Organizer
      The 25th Annual and International Meeting of tne Japanese Association for Animal Cell Technology
    • Place of Presentation
      Nagoya Congress Center (Aichi)
    • Year and Date
      2012-11-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] 骨格筋由来筋芽細胞の不均一播種での増殖過程の速度論的解析2012

    • Author(s)
      岸杏子
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] 集密的状態における網膜色素上皮細胞の成熟過程に対する速度論的解析2012

    • Author(s)
      寺西陽友
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] 積層筋芽細胞シートにおける細胞流動性の不均一性2012

    • Author(s)
      中村匡
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] 積層筋芽細胞シート内の血管内皮ネットワーク形成に対するサイトカイン添加効果2012

    • Author(s)
      長森英二
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] 積層細胞シート内の血管内皮ネットワーク形成や局在に影響を与える物理的要素2012

    • Author(s)
      長森英二
    • Organizer
      化学工学会第44回秋季大会
    • Place of Presentation
      東北大学川内北キャンパス(宮城県)
    • Year and Date
      2012-09-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] Induction of Human Mesenchymal Stem Cell Differentiation into A Cardiomyogenic- like Direction Based on Dynamic Cell Aggregate Behaviors2012

    • Author(s)
      Yuuki Ogawa
    • Organizer
      Tne 2nd International Symposium 01 Materials on Regenerative Medicine
    • Place of Presentation
      Taipei Medical University(Taipei, Taiwan)
    • Year and Date
      2012-08-30
    • Related Report
      2012 Annual Research Report
  • [Book] 細胞工学Vol. 32 No. 1, My Technology 「板状細胞集塊の作製とその利用」2013

    • Author(s)
      紀ノ岡正博
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2012 Annual Research Report
  • [Book] 幹細胞医療の実用化技術と産業展望「細胞シート内での血管内皮ネットワーク形成」2013

    • Author(s)
      紀ノ岡正博, 長森英二
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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