Development of "Displome" for industrial applications of cell surface display

• Project/Area Number: 24360342
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kobe University
• Principal Investigator: AKIHIKO Kondo 神戸大学, 工学(系)研究科(研究院), 教授 (40205547)
• Co-Investigator(Kenkyū-buntansha): TANAKA Tsutomu 神戸大学, 大学院工学研究科, 准教授 (90436551) ; OGINO Chiaki (MATSUMOTO Takuya / YAMADA Ryosuke) 神戸大学, 大学院工学研究科, 准教授 (00313693) ; 松本 拓也 神戸大学, 学内共同利用施設等, 助教 (40727161) ; 山田 亮祐 神戸大学, 自然科学系先端融合研究環, 助教 (40608626)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: バイオリファイナリー / 細胞表層提示 / バイオリファイなリー / オーム解析 / バイオマス

Outline of Final Research Achievements

The development of efficient utilization of renewable biomass resources is one of the important issues to overcome the environmental / energy problems. We have developed cell surface display system, which is a powerful tool due to endowing the biomass degrading activity on the host cells. Here, we developed comprehensive approaches to develop cell surface display applicable for other useful microorganisms. As a results, several kinds of anchor proteins have been developed and enables them to providing biomass degrading activity. In addition, using cell surface engineered microorganisms, direct production from biomass was achieved.

Research Products

• [Journal Article] Display of active beta-glucosidase on the surface of Schizosaccharomyces pombe cells using novel anchor proteins (2013)
  • Author(s): Tanaka, T., Matsumoto, S., Yamada, M., Yamada, R., Matsuda, F., Kondo, A.
  • Journal Title: Applied Microbiology and Biotechnology Volume: 97 Issue: 10 Pages: 4343-4352
  • DOI: 10.1007/s00253-013-4733-0
  • Peer Reviewed
• [Journal Article] Direct L-lysine production from cellobiose by Corynebacterium glutamicum displaying beta-glucosidase on its cell surface. (2013)
  • Author(s): Adachi, N., Takahashi, C., Murota, N., Yamaguchi, R., Tanaka, T., Kondo, A
  • Journal Title: Applied Microbiology and Biotechnology Volume: 97 Issue: 16 Pages: 7165-7172
  • DOI: 10.1007/s00253-013-5009-4
  • Peer Reviewed
• [Journal Article] Direct cadaverine production from cellobiose using beta-glucosidase displaying Escherichia coli (2013)
  • Author(s): Ikeda, N., Miyamoto, M., Adachi, N., Nakano, M., Tanaka, T., Kondo, A.
  • Journal Title: AMB Express Volume: 3 Issue: 1 Pages: 67-67
  • DOI: 10.1186/2191-0855-3-67
  • Peer Reviewed
• [Journal Article] Display of active beta-glucosidase on the cell surface of Schizosaccharomyces pombe using novel anchor proteins (2013)
  • Author(s): Tanaka, T., Matsumoto, S., Yamada, M., Yamada, R., Matsuda, F., Kondo, A.
  • Journal Title: Applied Microbiology and Biotechnology Volume: 97 Pages: 4343-4352
  • Peer Reviewed
• [Journal Article] (Recent developments in yeast cell surface display toward extended applications in biotechnology., (2012)
  • Author(s): Tanaka, T., Yamada R., Ogino, C., Kondo, A.
  • Journal Title: Applied Microbiology and Biotechnology Volume: 95 Pages: 577-591
  • Peer Reviewed
• [Presentation] βグルコシダーゼ提示大腸菌を用いたセロビオースからのカダべリン生産 (2014)
  • Author(s): 池田直樹、田中勉、近藤昭彦
  • Organizer: 化学工学会第79年会
  • Place of Presentation: 岐阜大学
• [Presentation] β-グルコシダーゼ表層提示 Corynebacterium glutamicumを用いたセロビオースからのリジン生産 (2013)
  • Author(s): 足立典子、田中勉、近藤昭彦
  • Organizer: 化学工学会第45回秋季大会
  • Place of Presentation: 岡山大学
• [Presentation] β-グルコシダーゼ提示大腸菌を用いたプトレシン生産経路の構築 (2013)
  • Author(s): 池田直樹、宮本茉里、田中勉、近藤昭彦
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場
• [Presentation] β-グルコシダーゼ発現Corynebacterium glutamicumを用いたセロビオースからのリジン生産 (2013)
  • Author(s): 足立典子・田中 勉・近藤昭彦
  • Organizer: 化学工学会第78年会
  • Place of Presentation: 大阪大学
• [Presentation] β-グルコシダーゼ発現Corynebacterium glutamicum を用いたセロビオースからのリジン生産 (2012)
  • Author(s): 足立典子, 小野尚子, 平田有希, 高橋千尋, 岡井直子, 田中勉, 近藤昭彦
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場