| Budget Amount *help |
¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Outline of Final Research Achievements |
Antibody is being applied for a next generation molecular targeting drug, and modified versions of antibodies, such as single chain antibodies, are also being developed. We have established an in vitro antibody generation system using a hypermutating chicken B cell line, DT40. In this study, we established a novel animal display system, in which any antibody or antibody variant of interest, even though it is derived from the other technology, can be introduced and expressed as a chimeric antibody with the human IgG1 constant region on DT40 cells, and then can be improved in its affinity with the mutation machinery of DT40 cells. This study will be useful for creating antibodies that have valuable activities as antibody drugs.
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