| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Outline of Final Research Achievements |
Non-LTR retrotransposon, so called Long Interspersed Nuclear Elements (LINE), is moved actively in most eukaryotic cells, but many processes involved in its transposition is largely unknown. In this study, we analyzed the regulation mechanisms of translation of open reading frame 1 protein (ORF1p) and open reading frame 2 protein (ORF2p) using human L1 element. In addition, we here revealed how ORF1p and ORF2p form the functional ribonucleoprotein (RNP) complex, and moves into the nucleoli, using rDNA specific LINE (R elements), R1, R2 and R7. Furthermore, we have clarified molecular mechanisms underlying how the LINE unit interacts and digests the target sequence correctly, and how the LINE mRNA binds to the target site, and reverse transcribe occurs from the correct site, using the R7 element.
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