| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Outline of Final Research Achievements |
Although centromere establishment at the novel DNA region without any sequence specificity has been proven inductively, its mechanism has been poorly elucidated molecularly due to the difficulty in the retrospective analysis. Therefore, we investigated the molecular mechanism of a phenomenon in the fission yeast experimental system in which a functionally-insufficient neocentromere, or the new-born centromere at ectopic position, gets matured in generations reproducibly. Our results indicated that functional insufficiency of the neocentromere comes from the poor association of centromere-specific histone CENP-A and its molecular chaperone Scm3, which is recovered upon maturation, and the recovery is attributable to the paucity of histone H2A.Z caused by the heterochromatin adjacency and/or the chromosomal position effect.
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