| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
We analyzed the function of a plant-unique RAB5 GTPsae, ARA6, and found that ARA6 negatively regulated the vacuolar trafficking pathway counteracting conventional RAB5 GTPases. We also revealed that this counteracting function was fulfilled through the function of an effector protein of ARA6, PUF2. We also tried to reconstruct molecular evolution of a plant-specific membrane fusion machinery, VAMP727, and found that functional conversion of VAMP727 from the ancestral function of VAMP72 members was mediated by two-step events: acquisition of an insertion sequence in the N-terminal longin domain followed by acidification of the inserted region.
Furthermore, we systematically analyzed RAB GTPases and SNARE molecules in the liverwort, Marchantia polymorpha, to collect information on the membrane trafficking system in the basal land plant.
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