Studies on diversification of membrane trafficking pathways in plants
| Project/Area Number |
24370019
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
UEDA TAKASHI 東京大学, 理学(系)研究科(研究院), 准教授 (10311333)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 膜交通 / 進化 / RAB GTPase / SNARE / Rab GTPase / 多様化 |
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| Outline of Final Research Achievements |
We analyzed the function of a plant-unique RAB5 GTPsae, ARA6, and found that ARA6 negatively regulated the vacuolar trafficking pathway counteracting conventional RAB5 GTPases. We also revealed that this counteracting function was fulfilled through the function of an effector protein of ARA6, PUF2. We also tried to reconstruct molecular evolution of a plant-specific membrane fusion machinery, VAMP727, and found that functional conversion of VAMP727 from the ancestral function of VAMP72 members was mediated by two-step events: acquisition of an insertion sequence in the N-terminal longin domain followed by acidification of the inserted region.
Furthermore, we systematically analyzed RAB GTPases and SNARE molecules in the liverwort, Marchantia polymorpha, to collect information on the membrane trafficking system in the basal land plant.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Molecular analysis and localization of CaARA7 a conventional RAB5 GTPase homolog from characean algae2015
Author(s)
Hoepflinger, M.C., Geretschlaeger, A., Sommer, A., Hoeftberger, M., Tenhaken, R., Ueda, T., and Foissner, I.
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Journal Title
Traffic
Volume: in press
Issue: 5
Pages: 534-554
DOI
Related Report
Peer Reviewed
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[Journal Article] Rab7 activation by the MON1-CCZ1 complex is essential for PVC-to-vacuole trafficking and plant growth in Arabidopsis2014
Author(s)
Cui, Y., Zhao, Q., Gao, C., DingY., Zeng, Y., Ueda, T., Nakano, A., and Jiang, L.
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Journal Title
Plant Cell
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Sex-specific post-translational regulation of the gamete fusogen GCSI in the isogamous volvocine alga Gonium pectorale.2014
Author(s)
Kawai-Toyooka H, Mori T, Hamaji T, Suzuki M, Olson BJ, Uemura T, Ueda T, Nakano A, Toyoda A, Fujiyama A, Nozaki H
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Journal Title
EUkaryotic Cell
Volume: (in press)
Issue: 5
Pages: 648-656
DOI
Related Report
Peer Reviewed
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[Journal Article] Interactomics of Qa-SNARE in Arabidopsis thaliana2014
Author(s)
Fujiwara, M., Uemura, T., Ebine, K., Nishimori, Y., Ueda, T., Nakano, A., Sato, MH. and Fukao, Y.
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Journal Title
Plant Cell Phys.
Volume: 55
Issue: 4
Pages: 781-789
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A unique HEAT repeat-containing protein SHOOT GRAVITROPISM 6 is involved in vacuolar membrane dynamics in gravity sensing cells of Arabidopsis inflorescence stem2014
Author(s)
Hashiguchi, Y., Yano, D., Nagafusa, K., Kato, T., Saito, C., Uemura, T., Ueda, T., Nakano, A., Tasaka, M. and Morita, MT
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Journal Title
Plant Cell Phys.
Volume: 55
Issue: 4
Pages: 811-822
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular and biochemical analysis of the first ARA6 homolog, a Rab5 GTPase, from green algae2013
Author(s)
Hoepflinger, M., Geretschlaeger, A., Hoeftberger, M., Sommer, A., Nishiyama, T., Sakayama, Hammerl, P., H., Tenhaken, R., Ueda, T., Foissner I.
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Journal Title
J. Exp. Bot.
Volume: 64
Issue: 18
Pages: 5553-68
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular mechanism of strigolactone perception by DWARF142013
Author(s)
Hidemitsu Nakamura, Yau-Lin Xue, Takuya Miyakawa, Feng Hou, Hui-Min Qin, Kosuke Fukui, Xuan Shi, Emi Ito, Shinsaku Ito, Seung-Hyun Park, Yumiko Miyauchi, Atsuko Asano, Naoya Totsuka, Takashi Ueda, Masaru Tanakura & Tadao Asami
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Journal Title
Nature Communications
Volume: 4
Issue: 1
Pages: 2613-2613
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A guanine nucleotide exchange factor for Rab5 proteins is essential for intracellular transport of the proglutelin from the Golgi apparatus to the protein storage vacuole in rice endosperm2013
Author(s)
Fukuda, M., Wen, L., Satoh-Cruz, M., Kawagoe, Y., Nagamura, Y., Okita, TW., Washida, H., Sugino, A., Ishino, S., Ishino, Y., Ogawa, M., Sunada, M., Ueda, T., and Kumamaru, T.
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Journal Title
Plant Phys.
Volume: 25
Pages: 663-674
Related Report
Peer Reviewed
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