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Experimental evaluation of diversification process of cyanobacterial circadian output systems

Research Project

Project/Area Number 24370020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto University

Principal Investigator

OYAMA Tokitaka  京都大学, 理学(系)研究科(研究院), 准教授 (30324396)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords概日時計 / シアノバクテリア / 遺伝子発現 / 時計タンパク質 / 多様性
Outline of Final Research Achievements

Cyanobacterial circadian clock systems are uncomplicated; the circadian oscillator is composed of only three clock proteins, and its output is the circadian regulation of gene expression. Among various cyanobacteria, circadian clock system is highly conserved but several groups lack its components. In order to elucidate diversification of the clock components at functional and physiological levels, novel experimental methods were developed using the model cyanobacterium, Synechococcus PCC 7942 as a research platform for chronobiology. Then, detailed analyses for behavior/function of clock-related proteins were achieved.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (8 results)

All 2015 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (6 results)

  • [Journal Article] Effects of adenylates on the circadian interaction of KaiB with the KaiC complex in the reconstituted cyanobacterial Kai protein oscillator2014

    • Author(s)
      Goda K, Kondo T, Oyama T
    • Journal Title

      Biosci., Biotech., Biochem.

      Volume: 78 Issue: 11 Pages: 1833-1838

    • DOI

      10.1080/09168451.2014.940833

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Theophylline-dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 79422013

    • Author(s)
      Yoichi Nakahira, Atsushi Ogawa, Hiroyuki Asano, Tokitaka Oyama, Yuzuru Tozawa
    • Journal Title

      Plant Cell Physiol.

      Volume: 54 Issue: 10 Pages: 1724-1735

    • DOI

      10.1093/pcp/pct115

    • NAID

      40019838886

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] シアノバクテリア概日時計タンパク質 KaiB の分子機能とアミノ酸配列の種間比較2015

    • Author(s)
      廣田周平,北川徳明,浅野宏幸,小山時隆
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 海洋性シアノバクテリア Synechococcus, Prochlorococcus における kaiB 遺伝子の機能多様化の解析2014

    • Author(s)
      北川 徳明, 浅野 宏幸, 廣田 周平, 小山 時隆
    • Organizer
      第22回日本時間生物学会学術大会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2014-11-08 – 2014-11-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 人工リボスイッチを用いたラン藻のための新規遺伝子発現制御技術2014

    • Author(s)
      中平 洋一、小川 敦司、浅野 宏幸、小山 時隆、戸澤 譲
    • Organizer
      日本分子生物学会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 人工リボスイッチを基盤としたラン藻のための新規遺伝子発現制御技術2013

    • Author(s)
      中平 洋一、小川 敦司、浅野 宏幸、小山 時隆、戸澤 譲
    • Organizer
      日本植物生理学会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] 高等植物の細胞概日リズム特性2012

    • Author(s)
      小山時隆
    • Organizer
      第19回日本時間生物学会学術大会
    • Place of Presentation
      北海道大学学術交流会館(北海道)
    • Year and Date
      2012-09-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] 海洋性シアノバクテリアProchlorococcusの時計遺伝子機能の推定2012

    • Author(s)
      浅野宏幸, 六車一志, 小山時隆
    • Organizer
      第19回日本時間生物学会学術大会
    • Place of Presentation
      北海道大学学術交流会館(北海道)
    • Year and Date
      2012-09-15
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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