Similarities and differences of molecular mechanisms on the regulation of meristematic activity

• Project/Area Number: 24370024
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kumamoto University
• Principal Investigator: SAWA Shinichiro 熊本大学, 自然科学研究科, 教授 (00315748)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2014: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: CLE pepitde signaling / 分裂組織 / 植物 / CLEペプチド / CLE / シロイヌナズナ / ペプチド

Outline of Final Research Achievements

BAM1 mainly function in root tissues, but clv1 does not. On the other hand, CLV1 functions but BAM1 has minor functions in the shoot apex. As for the RPK2, it functions with PUB4 in root tissues, but PUB4 has different functions in shoot tissues. CLEN3 functions only in root tissues functioning between BAM1 and MAPKKK.
In this analyses, we could find that there are similarities and differences on the molecular mechanisms regulating meristematic activities between shoot apical meristem and root apical meristem.

Research Products

• [Int'l Joint Research] ゲント大学(ベルギー)
• [Int'l Joint Research] デューク大学(米国)
• [Journal Article] Three-dimensional imaging of plant organs using a simple and rapid transparency technique. (2016)
  • Author(s): Hasegawa J, Sakamoto Y, Nakagami S, Aida M, Sawa S, Matsunaga S
  • Journal Title: Plant Cell Physiol Volume: 57 Issue: 3 Pages: 462-472
  • DOI: 10.1093/pcp/pcw027
  • NAID: 120005756556
  • Peer Reviewed / Open Access
• [Journal Article] The Naming of Names: Guidelines for Gene Nomenclature in Marchantia (2016)
  • Author(s): Bowman, J., Araki, T., Arteaga-Vazquez, M., Berger, F., Dolan, L., Haseloff, J., Ishizaki, K., Kyozuka, J., Lin, S.S., Nagasaki, H., Nakagami, H., Nakajima, K., Nakamura, Y., Ohashi-Ito, K., Sawa, S., Shimamura, M., Solano, R., Tsukaya, H., Ueda, T., Watanabe, Y., Yamato, K.T., Zachgo, S., and Kohchi, T.
  • Journal Title: Plnat cell Physiology Volume: 57 Issue: 2 Pages: 257-261
  • DOI: 10.1093/pcp/pcv193
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chemotaxis assay of plant-parasitic nematodes on a gel-filled microchannel device (2015)
  • Author(s): Hida, H., Nishiyama, H., Sawa, S., Higashiyama, T., Arata, H.
  • Journal Title: Sensors and Actuators Volume: B 221 Pages: 1483-1491
  • DOI: 10.1016/j.snb.2015.07.081
  • Peer Reviewed
• [Journal Article] BAM 1 and RECEPTOR-LIKE PROTEIN KINASE 2 constitute a signaling pathway and modulate CLE peptide-triggered growth inhibition in Arabidopsis root. (2015)
  • Author(s): Shimizu N, Ishida T, Yamada M, Shigenobu S, Tabata R, Kinoshita A, Yamaguchi K, Hasebe M, Mitsumasu K, Sawa S,
  • Journal Title: New phytologist Volume: 208 Issue: 4 Pages: 1104-1113
  • DOI: 10.1111/nph.13520
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 2.Plant peptide hormone signaling. (2015)
  • Author(s): Motomitsu A, Sawa S, Ishida T
  • Journal Title: Essays in Biochemistry Volume: 58 Pages: 115-131
  • DOI: 10.1042/bse0580115
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of parasitism of plant-parasitic nematodes at apoplast. (2015)
  • Author(s): Mitsumasu, K., Nishiyama, H., Nakagami, S., Kanemaru, Y. and Sawa, S
  • Journal Title: Jap. J. Pesticide Sci Volume: 40 Pages: 44-51
  • NAID: 130005096498
  • Peer Reviewed
• [Journal Article] Mystery in genetics: PUB4 gives a clue to the complex mechanism of CLV signaling pathway in the shoot apical meristem (2015)
  • Author(s): Kinoshita, A., Seo, M., Kamiya, Y., and Sawa, S.
  • Journal Title: Plant Signaling & Behavior Volume: 10:6 Issue: 6 Pages: e1028707-e1028707
  • DOI: 10.1080/15592324.2015.1028707
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Protocol for root-knot nematode culture by a hydroponic system and nematode inoculation to <i>Arabidopsi</i><i>s </i> (2015)
  • Author(s): Nishiyama, H., Ngan, B. T., Nagakami, S., Ejima, C., Ishida, T., and Sawa, S.
  • Journal Title: Nematological Research (Japanese Journal of Nematology) Volume: 45 Issue: 1 Pages: 45-49
  • DOI: 10.3725/jjn.45.45
  • NAID: 130005118607
  • ISSN: 0919-6765, 1882-3408
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A plant U-box protein, PUB4, regulates asymmetric cell division and cell proliferation in the root meristem. (2015)
  • Author(s): Kinoshita, A, Hove, C.A., Tabata, R., Yamada, M., Shimizu, N., Ishida, T., Yamaguchi K., Shigenobu, S., Takebayashi, Y., Iuchi, S., Kobayashi, M., Kurata, T., Wada, T., Seo, M., Hasebe, M., Bilou, I., Fukuda, H., Scheres, B., Heidstra, R., Sawa, S.
  • Journal Title: Development Volume: 142 Issue: 3 Pages: 444-453
  • DOI: 10.1242/dev.113167
  • Peer Reviewed / Open Access
• [Journal Article] Heterotrimeric G proteins control stem cell proliferation through CLAVATA signaling in Arabidopsis. (2014)
  • Author(s): Ishida, T., Tabata, R., Yamada, M., Aida, M., Mitsumasu, K., Fujiwara, M., Yamaguchi, K., Shigenobu, S., Higuchi, M., Tsuji, H., Shimamoto, K., Hasebe, M., Fukuda, H., Sawa, S.
  • Journal Title: EMBO rep. Volume: 15 Issue: 11 Pages: 1202-1209
  • DOI: 10.15252/embr.201438660
  • Peer Reviewed
• [Journal Article] Evolutionarily conserved CLE peptide signaling in plant development, symbiosis, and parasitism. (2013)
  • Author(s): Miyawaki, K.,et al.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 16 Issue: 5 Pages: 598-606
  • DOI: 10.1016/j.pbi.2013.08.008
  • Peer Reviewed
• [Journal Article] SUPPRESSOR OF LLP1 1-mediated C-terminal processing is critical for CLE19 peptide activity. (2013)
  • Author(s): Takayuki Tamaki
  • Journal Title: The Plant Journal Volume: 76 Issue: 6 Pages: 970-981
  • DOI: 10.1111/tpj.12349
  • Peer Reviewed
• [Journal Article] Identification of an EMS-induced causal mutation in a gene required forboron-mediated root development by low-coverage genome re-sequencing inArabidopsis (2013)
  • Author(s): Tabata, R. , Kamiya, T. , ShigenobuS., Yamaguchi K. , Yamada, M. ,Hasebe, M. , Fujiwara, T., andSawa, S
  • Journal Title: Plant Signaling & Behavior. Volume: 8
  • Peer Reviewed
• [Journal Article] Synergistic Interaction of CLAVATA1, CLAVATA2, and RECEPTOR-1 LIKE PROTEIN2 KINASE 2 1n Cyst Nematode Parasitism of Arabidopsis. (2013)
  • Author(s): Replogle, A., Wang, J., Paolillo, V. , Smeda, J. , Kinoshita, A. , Durbak, A., Tax, F. , Wang, X., Sawa, S. , and Mitchum, M. G.
  • Journal Title: Mol Plant Microbe Interact Volume: 26 Pages: 87-96
  • Peer Reviewed
• [Journal Article] CLAVATA3 regulates meristem size in Arabidopsis (2012)
  • Author(s): Sawa, S
  • Journal Title: HANDBOOK OF BIOLOGICALLY ACTIVE PEPTIDES. Ed. byA亅. Kastin, Elsevier Pages: 1-4
  • Peer Reviewed
• [Journal Article] The function of CLE and other plant peptide hormones in root developmeNt (2012)
  • Author(s): Yamada, M., and Sawa, S
  • Journal Title: Curr. Opin. Plant Biol Volume: 16 Pages: 1-6
  • Peer Reviewed
• [Presentation] Analysis of Arabidopsis attractant of plant parasitic nematode, M. incognita. (2016)
  • Author(s): Shinichiro Sawa
  • Organizer: 第57回日本植物生理学会
  • Place of Presentation: 岩手大学上田キャンパス
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] “TOMEI”, a simple and quick clearing method for plant tissues (2015)
  • Author(s): Satoru Nakagami 3, Yuki Katsuyama 2, Shinichiro Sawa 3, Sachihiro Matsunaga
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium and Technical Workshop 2015
  • Place of Presentation: 名古屋大学野依記念学術交流館
  • Year and Date: 2015-08-27
  • Int'l Joint Research
• [Presentation] Heterotrimeric G proteins は CLAVATA シグナル伝達経路と協 調的に機能し茎頂分裂組織におけ る細胞増殖の制御を行う (2015)
  • Author(s): 石田喬志 1 ，田畑亮 1 ，山田昌史 2 ，相 田光宏 3 ，光増可奈子 1 ，樋口雅之 3 ， 辻寛之 3 ，島本功 3 ，澤進一郎 1
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学 世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Molecular dissection of CLE signaling pathway in Arabidopsis (2015)
  • Author(s): Shinichiro Sawa
  • Organizer: Cambridge University seminar
  • Place of Presentation: Cambridge University
  • Year and Date: 2015-03-02
  • Invited
• [Presentation] Comprehensive genetic analysis of CLV3 downstream pathway in Arabidopsis. (2014)
  • Author(s): Shinichiro Sawa
  • Organizer: 第４７回日本発生生物学会
  • Place of Presentation: WINC AICHI
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] 植物感染性線虫の感染分子機構の解析 (2014)
  • Author(s): 澤進一郎、光増可奈子
  • Organizer: 日本農薬学会
  • Place of Presentation: 京都大学
• [Presentation] Carboxypeptidase in CLE peptide maturation step,and CLE signaling molecules in Arabidopsis (2013)
  • Author(s): Shinichiro Sawa
  • Organizer: 21ST Conference of the International Plant Growth Substances Association
  • Place of Presentation: The Shanghai International Conference Center(China)
• [Presentation] シロイヌナズナの幹細胞活性を規定するCLV3ペプチドホルモンシグナル伝達因子の順遺伝学による網羅的探索 (2013)
  • Author(s): 澤進一郎,志水法子,江島千佳,西山英孝,石田喬志
  • Organizer: 日本生化学大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Participation of the cell wall interface in intercellular signaling in plants (2013)
  • Author(s): Shinichiro Sawa
  • Organizer: International Cell Wall Integrity Symposium
  • Place of Presentation: KKR Kamakura Wakamiya
• [Book] 植物ゲノム解析、次世代シーケンサー活用術 (2016)
  • Author(s): 重信秀治、澤進一郎、栗原志、持田恵一、上原（山口）由紀子、高木宏樹、阿部陽、寺内良平、関原明、高橋聡史
  • Publisher: 化学同人
• [Book] 細胞外空間（細胞壁）に注目した植物寄生性線虫の感染分子機構 (2016)
  • Author(s): 光増可奈子，西山英孝，中上知，金丸由実，澤進一郎
  • Publisher: 日本農薬学会誌
• [Book] Analysis of attractant of plant parasitic nematode (2016)
  • Author(s): Sawa, S
  • Publisher: Soy Protein Research
• [Remarks]
  • URL: http://www.sci.kumamoto-u.ac.jp/~sawa/