The extrinsic proteins of photosystem II: structure, function and evolution
| Project/Area Number |
24370025
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
TOMO Tatsuya 東京理科大学, 理学部, 教授 (60300886)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Takumi 名古屋大学, 理学(系)研究科, 教授 (60241246)
MINO Hiroyuki 名古屋大学, 理学(系)研究科, 准教授 (70300902)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | 光化学 / 酸素発生 / 多様性 / 光化学系II / 表在性タンパク質 / 光合成 / 光化学系 |
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| Outline of Final Research Achievements |
The photosynthetic alga has a highly diverse in species. The extrinsic proteins of photosystem II (PS II) are also rich in diversity. To clarify the role of extrinsic proteins, we successfully isolated PS II complex from cyanobacteria, red alga, diatom and Symbiodinium. Our analyses showed the PsbO and PsbV affect the secondary structure of core subunits of PS II by Fourier transform infrared spectroscopy. PS II isolated from red alga, which is primitive red alga, contained fourth extrinsic proteins, PsbQ′, which role had not been elucidated yet. We did the measurements of redox potential of secondary electron transfer component of PS II (QA) to find out the role of this subunit. We observed the positive potential shit of QA after reconstitution of PsbQ’ to cyanobacterial PS II. This result indicates the role of QA reduces the yield of the triplet chlorophyll by direct charge recombination. As a result, this leads to the decrease of singlet oxygen in the red alga.
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Report
(4 results)
Research Products
(108 results)
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[Journal Article] Luminescence of singlet oxygen in photosystem II complexes isolated from cyanobacterium Synechocystis sp. PCC6803 containing monovinyl or divinyl chlorophyll a.2012
Author(s)
Tomo, T., Kusakabe, H., Nagao, R., Ito, H., Tanaka, A., Akimoto, S., Mimuro, M. and Okazaki, S
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Journal Title
Biochim Biophys Acta.
Volume: (in press)
Issue: 8
Pages: 1299-1305
DOI
Related Report
Peer Reviewed
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[Presentation] Development of an artificial light-driven water splitting nano-device using photosynthetic proteins and metal nanoparticles2014
Author(s)
Kazuki Tahara, Kousuke Kawahara, Keisuke Namie, Natsuko Inoue, Ryo Nagao, Yuki Kato, Tatsuya Tomo, Yutaka Shibata, Hiroshi Fukumura, Yasuhiro Kashino, Takumi Noguch
Organizer
第52回日本生物物理学会年会
Place of Presentation
札幌コンベンションセンター
Year and Date
2014-09-25 – 2014-09-27
Related Report
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[Presentation] 藻類の多様性と光化学系2014
Author(s)
鞆 達也
Organizer
第５回日本光合成学会年会
Place of Presentation
奈良・近畿大学農
Year and Date
2014-06-02 – 2014-06-07
Related Report
Invited
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