Molecular mechanisms of sexual plasticity in adult medaka gonads
Project/Area Number |
24370028
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Morphology/Structure
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Research Institution | Ehime University |
Principal Investigator |
NAGAHAMA Yoshitaka 愛媛大学, 南予水産研究センター, 特定教員(教授) (50113428)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2012: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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Keywords | 雌雄異体魚 / メダカ成魚 / 性的可塑性 / 性転換 / 生殖幹細胞 / 生殖腺体細胞 / エストロゲン / 芳香化酵素阻害剤 / メダカ / 成熟魚 / 遺伝子発現 |
Outline of Final Research Achievements |
In all gonochoristic vertebrates, the sexual plasticity of the gonads is not retained after the completion of sex differentiation. Here, we studied whether medaka gonads maintain sexual plasticity until adulthood. The depletion of estrogen resulted in a functional female-to-male sex reversal in sexually mature females. The sex-reserved fish showed a typical male pattern of estrogen levels, secondary sex characteristics, and male-like sex behavior. On the other hand, estrogen treatment in adult males undergoing spermatogenesis induced male-to-female sex reversal, producing fertile eggs. In situ hybridization of medaka gonads during female-to-male and male-to-female sex reversals indicated that cysts on the periphery portions of adult gonads are the origins of germ cells and their surrounding somatic cells in the newly formed testicular and ovarian tissues. Gonochoristic fish maintain their sexual plasticity until adulthood and estrogen plays critical roles in maintaing phenotypic sex.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Estrogen oversees the maintenance of the female genetic program in terminally differentiated gonochorists.2013
Author(s)
Paul-Prasanth, B., Bhandari, B. K., Kobayashi, T., Horiguchi, R., Kobayashi, Y., Nakamoto. M., Shibata, Y., Sakai, F., Nakamura, M., Nagahama, Y.
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Journal Title
Scientific Reports
Volume: 3
Issue: 1
Pages: 2862-2872
DOI
Related Report
Peer Reviewed
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