| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
Sex determination mechanisms can broadly be categorized by either a genotypic or environmentally driven mechanism. We investigated environmental sex determination (ESD) mechanism using American alligator and watre flea (daphnia) as a models. We analyzed the thermosensitive cation channel, transient receptor potential (TRP), and its role during alligator temperature-dependent sex determination. Our electrophysiological and pharmacological approaches demonstrate that a thermal TRP is involved in the TSD in alligators and it directly activates male differentiation cascade. We also identified several juvenile hormone and ecdysone system-related genes, which are involved in sex determination pathways in Daphnia. The current results provide useful knowledge for understanding the mechanisms of sex determination system both in vertebrates and invertebrates.
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