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Analysis of brain neurons and molecules involved in diapause control in flies.

Research Project

Project/Area Number 24370032
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionOsaka City University

Principal Investigator

Shiga Sakiko  大阪市立大学, 大学院理学研究科, 教授 (90254383)

Co-Investigator(Kenkyū-buntansha) YASUYAMA Koji  川崎医科大学, 医学部, 教授 (60148690)
GOTO Shinsuke  大阪市立大学, 大学院理学研究科, 准教授 (70347483)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywords休眠 / 光周性 / トランスクリプトーム解析 / ハエ / 次世代シーケンサー解析 / 概日時計
Outline of Final Research Achievements

We have studied neurons and molecules involved in diapause control in flies. Previous studies have shown 2 types of neurosecretory cells, the pars intercerebralis neurons (PI) and pars lateralis neurons (PL), and small ventral lateral neurons (sLNv) as circadian clock neurons are important for photoperiodic control of diapause.
Severance of fiber tracts suggest that the PL with axons in the posterior lateral tract (PLT) is crucial for diapause control by suppressing ovarian development under short days. We found a gene sequence of which mRNA levels increased in 3 days after transferring to short days. The results suggest that sLNv submit different signals between short days and long days to the PL, which directly control ovarian development by changing gene expression.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (24 results)

All 2016 2015 2014 2013 2012

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Acknowledgement Compliant: 2 results) Presentation (14 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Neuroanatomy of the pars intercerebralis neurons in the blow fly Protophormia terraenovae with an interest in connections with pigment-dispersing factor-immunoreactive neurons.2015

    • Author(s)
      Yasuyama, K., Hase, H., Shiga, S.
    • Journal Title

      Cell and Tissue Research

      Volume: 362 Issue: 1 Pages: 33-43

    • DOI

      10.1007/s00441-015-2192-x

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Pars intercerebralis promotes oviposition in the bean bug, Riptortus pedestris (Heteroptera: Alydidae).2014

    • Author(s)
      Shimokawa, K., Numata, H., Shiga, S.
    • Journal Title

      Applied Entomology and Zoology

      Volume: 49 Issue: 4 Pages: 525-528

    • DOI

      10.1007/s13355-014-0281-z

    • NAID

      40020278689

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The circatidal rhythm persists without the optic lobe in the mangrove cricket Apteronemobius asahinai.2014

    • Author(s)
      Takekata, H., Numata, H., Shiga, S.
    • Journal Title

      Joumal of Biological Rhythms

      Volume: 29 Issue: 1 Pages: 28-37

    • DOI

      10.1177/0748730413516309

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Silencing the circadian clock gene Clock using RNAi reveals dissociation of the circatidal clock from the circadian clock in the mangrove cricket.2014

    • Author(s)
      Takekata, H., Numata, H., Shiga, S., Goto, S.G.
    • Journal Title

      Journal of Insect Physiology

      Volume: 68 Pages: 16-22

    • DOI

      10.1016/j.jinsphys.2014.06.012

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Both the anterior and posterior eyes function as photoreceptors for photoperiodic termination of diapause in the two-spotted spider mite.2014

    • Author(s)
      Hori, Y., Numata, H., Shiga, S., Goto, S.G.
    • Journal Title

      Journal of Comparative Physiology A

      Volume: 200 Issue: 2 Pages: 161-167

    • DOI

      10.1007/s00359-013-0872-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The involvement of the brain region containing pigment-dispersing factor-immunoreactive neurons in the photoperiodic response of the bean bug Riptortus pedestris.2014

    • Author(s)
      Tomoko Ikeno et al.
    • Journal Title

      Journal of Experimental Biology

      Volume: 217 Pages: 453-462

    • DOI

      10.1242/jeb.091801

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of ablation of the pars intercerebralis on ecdysteroid quantities and yolk protein expression in the blow fly, Protophormia terraenovae.2013

    • Author(s)
      Ayako Tanaka et al.
    • Journal Title

      Physiological Entomology

      Volume: 38 Issue: 3 Pages: 192-201

    • DOI

      10.1111/phen.12012

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of the brain in photoperiodic regulation of juvenile hormone biosynthesis in the brown-winged green bug Plautia stali.2013

    • Author(s)
      Keiji Matsumoto et al.
    • Journal Title

      Journal of Insect Physiology

      Volume: 59 Issue: 4 Pages: 387-393

    • DOI

      10.1016/j.jinsphys.2013.01.007

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoperiodic plasticity in circadian clock neurons in insects2013

    • Author(s)
      Sakiko Shiga
    • Journal Title

      Frontiers in Invertebrate Physiology

      Volume: 4(In press)

    • DOI

      10.3389/fphys.2013.00069

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plausible neural circuitry for photoperiodism in the blow fly, protophormia terraenovae.2012

    • Author(s)
      Sakiko Shiga
    • Journal Title

      Acta Biologica Hungarica

      Volume: 63 Issue: Supplement 2 Pages: 36-47

    • DOI

      10.1556/abiol.63.2012.suppl.2.3

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] チャバネアオカメムシの幼若ホルモン合成抑制ペプチド2016

    • Author(s)
      松本圭司、後藤 慎介、志賀 向子、末次 克行、田中 良明、小滝 豊美、篠田徹郎
    • Organizer
      日本昆虫学会第76回大会 第60回日本応用動物昆虫学会 合同大会
    • Place of Presentation
      大阪府立大学(大阪府・堺市)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ホソヘリカメムシの光周性に関わる光受容器から脳への神経投射2016

    • Author(s)
      XIJILI、志賀向子
    • Organizer
      日本昆虫学会第76回大会 第60回日本応用動物昆虫学会 合同大会
    • Place of Presentation
      大阪府立大学(大阪府・堺市)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] シリアカニクバエの羽化ゲートの決定における脳と環状線の役割2015

    • Author(s)
      西村晃爾・志賀向子
    • Organizer
      (公社)日本動物学会第86回大会
    • Place of Presentation
      新潟コンベンションセンター(新潟県・新潟市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] ルリキンバエの脳における短日低温に応答する遺伝子の探索2015

    • Author(s)
      口開麻由・志賀向子
    • Organizer
      (公社)日本動物学会第86回大会
    • Place of Presentation
      新潟コンベンションセンター(新潟県・新潟市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Characterization and distribution of allatostatic factors in the brown-winged green bug Plautia stali2015

    • Author(s)
      Matsumoto K, Shiga S
    • Organizer
      The 9th International Congress of Comparative Physiology and Biochemistry From Molecules to Macrophysiology ICCPB 2015
    • Place of Presentation
      Jagielloniam University (ポーランド, Krakow)
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シリアカニクバエの成虫発育完了後の羽化抑制2014

    • Author(s)
      西村晃爾・志賀向子
    • Organizer
      (公社)日本動物学会第85回大会
    • Place of Presentation
      東北大学(宮城県・仙台市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 昆虫における光周性神経機構の解析2014

    • Author(s)
      志賀向子
    • Organizer
      (公社)日本動物学会第85回大会
    • Place of Presentation
      東北大学(宮城県・仙台市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] シリアカニクバエの成虫発育の完了時刻と羽化時刻2014

    • Author(s)
      西村晃爾・志賀向子
    • Organizer
      2014年(社)日本動物学会近畿支部研究発表会
    • Place of Presentation
      兵庫県立大学(兵庫県・姫路市)
    • Year and Date
      2014-05-11 – 2014-05-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 昆虫の休眠制御に関わる神経機構2013

    • Author(s)
      志賀向子
    • Organizer
      第57回日本応用動物昆虫学会
    • Place of Presentation
      日本大学生物資源科学部(神奈川県)(招待講演)
    • Year and Date
      2013-03-27
    • Related Report
      2012 Annual Research Report
  • [Presentation] Effect of severance of neural fibers in the brain on reproductive diapause in the blow fly, Protophormia terraenovae2013

    • Author(s)
      Hiroaki Hase et al.
    • Organizer
      the 35th annual meeting of Japanese society for Comparative Physiology and Biolchemisty
    • Place of Presentation
      Egret Himeji(兵庫県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ルキンバエの休眠・非休眠条件の脳におけるトランスクリプトーム解析2013

    • Author(s)
      長谷紘明他
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山大学(岡山県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 虫たちの多様なリズム―野外生態から脳へ―2013

    • Author(s)
      志賀向子
    • Organizer
      生物リズム若手研究者の集い
    • Place of Presentation
      四季の宿「ぽぷら」(山梨県)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ルリキンバエの休眠調節における脳間部とインスリンシグナルの役割2012

    • Author(s)
      志賀向子
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪大学(大阪府)
    • Year and Date
      2012-09-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] Morphological analysis of neuronal connections between pigment-dispersing factor-immunoreactive neurons and pars intercerebralis neurons in the blow fly, Protophormia terraeaovae.2012

    • Author(s)
      Hiroaki Hase
    • Organizer
      日本比較生理生化学会
    • Place of Presentation
      湘南国際村センター(神奈川県)
    • Year and Date
      2012-07-07
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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