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Unexploited habitat of microalgae - investigating biodiversity in the lower photic zone

Research Project

Project/Area Number 24370034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biodiversity/Systematics
Research InstitutionHokkaido University

Principal Investigator

Horiguchi Takeo  北海道大学, 理学(系)研究科(研究院), 教授 (20212201)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords鞭毛藻類 / 渦鞭毛藻類 / 有光層下部 / 底生性真核微生物 / 分類 / 分子系統解析 / 微細構造 / 底生性真核生物
Outline of Final Research Achievements

Sandy seabed at 30 - 50 m deep off Satsunan Islands in subtropical Japan was regarded as an unexploited habitat for microalgae and an intensive taxonomic study was performed on the samples from this region. Total of 92 culture strains (many of them are dinoflagellates) were established and morphological and phylogenetic investigations were made. As a result, in addition to 26 known species (13 genera), 44 species (20 genera) were recognized as possible novel taxa. For purpose of comparison, the samples from shallow waters were also studied and 86 strains were established. The outcome of these taxonomic works greatly contributed further understanding of species diversity of microalgae.
Using many culture strains established in this project, photosynthetic pigment compositions of both benthic and planktonic dinoflagellates were analyzed using HPLC. Only the benthic species possessed a dozen of additional pigments, suggesting that pigment compositions are linked to habitat types.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (35 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (13 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 12 results,  Open Access: 2 results,  Acknowledgement Compliant: 6 results) Presentation (21 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] GYMNOXANTHELLA RADIOLARIAE GEN. ET SP. NOV. (DINOPHYCEAE), A DINOFLAGELLATE SYMBIONT FROM SOLITARY POLYCYSTINE RADIOLARIANS2016

    • Author(s)
      T. Yuasa, T. Horiguchi, S. Mayama, O. Takahashi
    • Journal Title

      Journal of Phycology

      Volume: 52 Issue: 1 Pages: 89-104

    • DOI

      10.1111/jpy.12371

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Specificity of Chroomonas (Cryptophyceae) as a source of kleptochloroplast for Nusuttodinium aeruginosum (Dinophyceae).2016

    • Author(s)
      Ryo Onuma and Takeo Horiguchi
    • Journal Title

      Phycological Research

      Volume: 64 Pages: 35-43

    • NAID

      120005951953

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Taxonomy and phylogeny of Pyramimonas vacuolata sp. nov. (Pyramimonadales, Chlorophyta).2015

    • Author(s)
      Mohammad Azmal Hossain Bhuiyan, Daphne Georgina Faria, Takeo Horiguchi, Stuart D. Sym, Shoichiro Suda
    • Journal Title

      Phycologia

      Volume: 54 Issue: 4 Pages: 192-209

    • DOI

      10.2216/15-004.1

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genetic diversity of Moestrupia oblonga (Dinophyceae) from coastal areas of Okinawa Island, Japan.2015

    • Author(s)
      Danang Ambar Prabowo, M.M.R. Shah, Takeo Horiguchi, Shoichiro Suda
    • Journal Title

      Marine Biodiversity

      Volume: 46 Issue: 1 Pages: 197-209

    • DOI

      10.1007/s12526-015-0351-7

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Pigment compositions are linked to the habitat types in dinoflagellates2015

    • Author(s)
      Norico Yamada, Ayumi Tanaka, Takeo Horugichi
    • Journal Title

      Journal of Plant Research

      Volume: 128 Issue: 6 Pages: 923-932

    • DOI

      10.1007/s10265-015-0745-4

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Kleptochloroplast enlargement, karyoklepty and the distribtuion of the cryptomonad nucleus in Nusuttodinium (=Gymnodinium) aeruginosum (Dinophyceae).2015

    • Author(s)
      Ryo Onuma and Takeo Horiguchi
    • Journal Title

      Protist

      Volume: 166 Issue: 2 Pages: 177-195

    • DOI

      10.1016/j.protis.2015.01.004

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A new definition of Adenoides eludens, an unusual marine sand-dwelling dinoflagellate without cingulum, and Pseudoadenoides kofoidii, gen. et comb. nov. for the species formerly known as Adenoides eluden.2015

    • Author(s)
      Fernando Gomez, Ryo Onuma, Luis F. Artigas, Takeo Horiguchi
    • Journal Title

      European Journal of Phycology

      Volume: 50 Issue: 2 Pages: 125-138

    • DOI

      10.1080/09670262.2015.1009174

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pellucidodinium psammophilum gen. et sp. nov. and Nusuttodinium desymbiontum sp. nov. (Dinophyceae), two novel heterotrophs closely-related to kleptochloroplastidic dinoflagellates.2015

    • Author(s)
      Ryo Onuma, Kunihiko Watanabe and Takeo Horiguchi
    • Journal Title

      Phycologia

      Volume: 54 Issue: 2 Pages: 192-209

    • DOI

      10.2216/14-103.1

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Phylogeny of five species of Nusuttodinium gen. nov. (Dinophyceaae), a genus of unarmoured kelptoplastidic dinoflagelaltes.2014

    • Author(s)
      Yoshihito Takano, Haruyo Yamaguchi, Isao Inouye, Ojvind Moestrup and Takeo Horiguchi
    • Journal Title

      Protist

      Volume: 165 Issue: 6 Pages: 759-778

    • DOI

      10.1016/j.protis.2014.09.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Ankistrodinium armigerum sp. nov. (Dinophyceae), a new species of heterotrophic marine sand-dwelling dinoflagellate from Japan and Australia.2014

    • Author(s)
      Kunihiko Wantanabe, Yukiko Miyoshi, Fumiyasu Kubo, Ryo Onuma, Shauna Murray and Takeo Horiguchi
    • Journal Title

      Phycological Research

      Volume: 62 Issue: 2 Pages: 125-135

    • DOI

      10.1111/pre.12047

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] cPPB-aE is discovered from photosynthetic benthic dinoflagellates.2014

    • Author(s)
      Norico Yamada,Ayumi Tanaka,Takeo Horiguchi
    • Journal Title

      Journal of Phycology

      Volume: 50 Issue: 1 Pages: 101-107

    • DOI

      10.1111/jpy.12135

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphological transition in kleptochloroplasts after ingestion in the dinoflagellates, Amphidinium poecilochroum and Gymnodinium aeruginosum (Dinophyceae).2013

    • Author(s)
      Ryo Onuma and Takeo Horiguchi
    • Journal Title

      Protist

      Volume: 164 Issue: 5 Pages: 622-642

    • DOI

      10.1016/j.protis.2013.06.003

    • NAID

      120005348936

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BISPINODINIUM ANGELACEUM GEN. ET SP. NOV. (DINOPHYCEAE), A NEW SAND-DWELLING DINOFLAGELLATE FROM THE SEAFLOOR OFF MAGESHIMA ISLAND JAPAN2013

    • Author(s)
      N. Yamada, R. Terada, A. Tanaka, T. Horiguchi
    • Journal Title

      Journal of Phycology

      Volume: 49(受理済み論文としてオンライン公開中) Issue: 3 Pages: 555-569

    • DOI

      10.1111/jpy.12064

    • Related Report
      2012 Annual Research Report
  • [Presentation] Morphology and phylogeny of a new tidal pool dinoflagellate Bysmatrum sp. from South Africa.2016

    • Author(s)
      Mahmutjan Dawut, Stuart Sym, Shoichiro Suda and Takeo Horiguchi
    • Organizer
      第40回日本藻類学会
    • Place of Presentation
      日本歯科大学(東京都・千代田区)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] A novel benthic Heterocapsa species, closely related to Heterocapsa pseudotriquetra.2016

    • Author(s)
      Sohail K. Pinto, Ryuta Terada and Takeo Horiguchi
    • Organizer
      第40回日本藻類学会
    • Place of Presentation
      日本歯科大学(東京都・千代田区)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Marine benthic dinoflagellates - unveiling hidden biodiversity -2015

    • Author(s)
      Takeo Horiguchi
    • Organizer
      9th International Symposium of National Institute of Biological Resources.
    • Place of Presentation
      仁川(大韓民国)
    • Year and Date
      2015-10-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Diversity and phylogeny of the dinoflagellate genus Testudodinium2015

    • Author(s)
      Sohail K. Pinto, Ryuta Terada and Takeo Horiguchi
    • Organizer
      6th European Phycological Congress
    • Place of Presentation
      ロンドン (イギリス)
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two novel athecate benthic dinoflagellates from the seabed off Mageshima Island, Sub-tropical Japan2015

    • Author(s)
      Sohail K. Pinto, Ryuta Terada and Takeo Horiguchi
    • Organizer
      6th European Phycological Congress
    • Place of Presentation
      ロンドン (イギリス)
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 渦鞭毛藻類Nusuttodinium spp.における盗葉緑体拡大とクリプト藻核保持との関連2015

    • Author(s)
      大沼亮・堀口健雄
    • Organizer
      日本藻類学会第39回大会
    • Place of Presentation
      九州大学箱崎キャンパス,福岡市
    • Year and Date
      2015-03-20 – 2015-03-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] A novel benthic dinoflagellate species belonging to the genus Heterocapsa.2015

    • Author(s)
      Sohail Keegan Pinto, Ryuta Terada and Takeo Horiguchi
    • Organizer
      日本藻類学会第39回大会
    • Place of Presentation
      九州大学箱崎キャンパス,福岡市
    • Year and Date
      2015-03-20 – 2015-03-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Two novel dinoflagellates from the seabed off Mageshima Island, Kagoshima Pref., Subtropical Japan2014

    • Author(s)
      Sohail Keegan Pinto, Norico Yamada, Ayumi Tanaka, Ryuta Terada and Takeo Horiguchi
    • Organizer
      第78回日本植物学会
    • Place of Presentation
      明治大学生田校舎,川崎市
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] THE DIATOM-DERIVED CHLOROPLASTS IN DINOFLAGELLATES ARE ORIGINED FROM 8 FREE-LIVING DIATOMS2014

    • Author(s)
      Norico Yamada, Stuart D. Sym and Takeo Horiguchi
    • Organizer
      Protist 2014 - Joint ISEP/ISOP meeting
    • Place of Presentation
      Banff conference centre, Canada
    • Year and Date
      2014-08-03 – 2014-08-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] 石狩産海岸砂地性従属栄養渦鞭毛藻類の分類学的研究 II2013

    • Author(s)
      渡邊邦彦・堀口健雄
    • Organizer
      日本藻類学会第37回大会
    • Place of Presentation
      山梨大学(甲府市)
    • Year and Date
      2013-03-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] 薩南諸島付近の海底に生息する底生性渦鞭毛藻類の系統分類学的研究2012

    • Author(s)
      山田規子・寺田竜太・堀口健雄
    • Organizer
      日本藻類学会第36回大会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-07-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] 石狩産海岸砂地性従属栄養渦鞭毛藻類の分類学的研究2012

    • Author(s)
      渡邊邦彦・大沼亮・堀口健雄
    • Organizer
      日本藻類学会第36回大会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-07-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] Diversity of morphology and photosynthetic pigments in dinotoms, including those of two new species.

    • Author(s)
      Norico Yamada, Ayumi Tanaka, Stuart D. Sym and Takeo Horiguchi
    • Organizer
      10th International Phycological Congress
    • Place of Presentation
      The Renaissance Orlando, Orlando, FL, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Diversity of morphology and photosynthetic pigments in dinotoms, including those of two new species.

    • Author(s)
      Ryo Onuma and Takeo Horiguchi
    • Organizer
      10th International Phycological Congress
    • Place of Presentation
      The Renaissance Orlando, Orlando, FL, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Deep (30-50 m) sand-dwelling marine dinoflagellates.

    • Author(s)
      Takeo Horiguchi, Norico Yamada, Rui Moriya and Ryuta Terada
    • Organizer
      10th International Phycological Congress
    • Place of Presentation
      The Renaissance Orlando, Orlando, FL, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 渦鞭毛藻の光合成色素組成は生活形態に応じて獲得される。

    • Author(s)
      山田規子,田中歩,Stuart D. Sym,堀口健雄
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学,札幌市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Taxonomic studies on benthic marine dinoflagellates in Japan and South Africa.

    • Author(s)
      Takeo Horiguchi, Norico Yamada, Kunihiko Watanabe, Nanako Matsuzawa, Shoichiro Suda and Stuart D. Sym
    • Organizer
      28th Congress of the Phycological Society of Southern Africa
    • Place of Presentation
      Atlantic Beach Golf Club, Melkbosstrand, Cape Town, South Africa
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Diversity of endosymbiont diatoms and their pigment profiles in dinoflagellates harbouring a diatom-derived chloroplast.

    • Author(s)
      Norico Yamada, Ayumi Tanaka, Stuart D. Sym and Takeo Horiguchi
    • Organizer
      28th Congress of the Phycological Society of Southern Africa
    • Place of Presentation
      Atlantic Beach Golf Club, Melkbosstrand, Cape Town, South Africa
    • Related Report
      2013 Annual Research Report
  • [Presentation] Morphological transition of kleptochloroplast in the unarmoured dinoflagellates Amphidinium poecilochroum and Gymnodinium aeruginosum (Dinophyceae).

    • Author(s)
      Ryo Onuma and Takeo Horiguchi
    • Organizer
      28th Congress of the Phycological Society of Southern Africa
    • Place of Presentation
      Atlantic Beach Golf Club, Melkbosstrand, Cape Town, South Africa
    • Related Report
      2013 Annual Research Report
  • [Presentation] Morphology and phylogeny of three Amphidinium species (Dinophyceae).

    • Author(s)
      Clark Gen, Maiko Tamura and Takeo Horiguchi
    • Organizer
      日本藻類学会第38回大会
    • Place of Presentation
      東邦大学,船橋市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 底生性渦鞭毛藻における生活形態依存的に生成される光合成色素の多様性とその機能。

    • Author(s)
      山田規子,田中歩,高市真一,堀口健雄
    • Organizer
      日本藻類学会第38回大会
    • Place of Presentation
      東邦大学,船橋市
    • Related Report
      2013 Annual Research Report
  • [Book] MARINE BENTHIC DINOFLAGELLATES - UNVEILING THEIR WORLDWIDE BIODIVERSITY2014

    • Author(s)
      Mona Hoppenrath, Shauna A. Murray, Nicolas Chomerat and Takeo Horiguchi
    • Publisher
      Kleine Senckenberg-Reihe, Senckenberg, Stuttgart
    • Related Report
      2014 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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