| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Outline of Final Research Achievements |
Blue light illumination at 100 K induced a red-shift of the absorption maximum, indicating that an equilibrium state of three isomeric states, Rhodopsin, Batho and 9-cis isorhodopsin was established. When this crystal was warmed to 240K, the absorption maximum was blue-shifted. Accurate estimation of isomer composition under this condition suggested that Batho was converted to LM with no change in the contents of the other states. We collected diffraction data set and calculate the difference electron density maps. It indicates that the conformational changes are located at the vicinity of the active site upon formation the LM intermediate. The larger movements of helices that characterize meta formation are not initiated at the early stage of photocycle in squid rhodopsin, but occur during the transition from LM to Meta. We try to crystallize the Acid Meta state of squid rhodopsin and succeed to obtain some new crystals which can diffract up to high resolution.
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