Structural basis for N-linked glycosylation site occupancy
Project/Area Number |
24370047
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Kyushu University |
Principal Investigator |
KOHDA Daisuke 九州大学, 生体防御医学研究所, 教授 (80186618)
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Co-Investigator(Renkei-kenkyūsha) |
SHIMADA Atsushi (70391977)
MAYANAGI Kouta (50418571)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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Keywords | N型糖鎖修飾 / 翻訳後修飾 / X線結晶解析 / 膜タンパク質 / 糖転移酵素 / N型糖鎖修飾 / 古細菌 / 脂質結合型糖鎖 / 動的構造 / X線結晶解析 / NMR緩和時間解析 |
Outline of Final Research Achievements |
The addition of oligosaccharide chains on the asparagine residues in proteins is catalyzed by the membrane enzyme, oligosaccharyltransferase (OST). We determined the crystal structures of the C-terminal globular domains and the full-length OST protein from hyperthermophilic archaea. We also showed that the dynamic feature of the C-terminal globular domains was essential for the oligosaccharyl transfer activity. The crystal structure in the complex with the oligosaccharide acceptor substrate was determined using the tethering of a peptide containing the Asn-Val-Thr sequon to the protein through a disulfide bond. Toward the determination of the complex structure with the oligosaccharide donor substrate, we analyzed the chemical structures of archaeal lipid-linked oligosaccharides to synthesize the water-soluble analogues.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Crystal structure of the C-terminal globular domain of oligosaccharyltransferase from Archaeoglobus fulgidus at 1.75Å resolution.2012
Author(s)
Matsumoto, S., Igura, M., Nyirenda, J., Matsumoto. M, Yuzawa, S., Noda, N., Inagaki, F., Kohda, D.
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Journal Title
Biochemistry
Volume: 51
Issue: 20
Pages: 4157-4166
DOI
Related Report
Peer Reviewed
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