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Structural basis for N-linked glycosylation site occupancy

Research Project

Project/Area Number 24370047
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Structural biochemistry
Research InstitutionKyushu University

Principal Investigator

KOHDA Daisuke  九州大学, 生体防御医学研究所, 教授 (80186618)

Co-Investigator(Renkei-kenkyūsha) SHIMADA Atsushi   (70391977)
MAYANAGI Kouta   (50418571)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
KeywordsN型糖鎖修飾 / 翻訳後修飾 / X線結晶解析 / 膜タンパク質 / 糖転移酵素 / N型糖鎖修飾 / 古細菌 / 脂質結合型糖鎖 / 動的構造 / X線結晶解析 / NMR緩和時間解析
Outline of Final Research Achievements

The addition of oligosaccharide chains on the asparagine residues in proteins is catalyzed by the membrane enzyme, oligosaccharyltransferase (OST). We determined the crystal structures of the C-terminal globular domains and the full-length OST protein from hyperthermophilic archaea. We also showed that the dynamic feature of the C-terminal globular domains was essential for the oligosaccharyl transfer activity. The crystal structure in the complex with the oligosaccharide acceptor substrate was determined using the tethering of a peptide containing the Asn-Val-Thr sequon to the protein through a disulfide bond. Toward the determination of the complex structure with the oligosaccharide donor substrate, we analyzed the chemical structures of archaeal lipid-linked oligosaccharides to synthesize the water-soluble analogues.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (26 results)

All 2015 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Invited: 3 results) Book (2 results) Remarks (3 results)

  • [Journal Article] N-Glycosylation with Synthetic Undecaprenyl Pyrophosphate-Linked Oligosaccharide to Oligopeptides by PglB Oligosaccharyltransferase from Campylobacter jejuni2015

    • Author(s)
      Ishiwata A, Taguchi Y, Lee YJ, Watanabe T, Kohda D, Ito Y.
    • Journal Title

      Chembiochem

      Volume: 16 Issue: 5 Pages: 731-737

    • DOI

      10.1002/cbic.201402658

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structural elucidation of an asparagine-linked oligosaccharide from the hyperthermophilic archaeon, Pyrococcus furiosus.2014

    • Author(s)
      Fujinami D, Matsumoto M, Noguchi T, Sonomoto K, Kohda D
    • Journal Title

      Carbohydr Res

      Volume: 387C Pages: 30-36

    • DOI

      10.1016/j.carres.2014.01.021

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Basis of the Asparagine Side Chain Activation in N-Linked Protein Glycosylation2014

    • Author(s)
      松本俊介,神田大輔
    • Journal Title

      Seibutsu Butsuri

      Volume: 54 Issue: 3 Pages: 158-159

    • DOI

      10.2142/biophys.54.158

    • NAID

      130004719365

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2014 Annual Research Report
  • [Journal Article] Crystal structure of the C-terminal globular domain of the third paralog of the Archaeoglobus fulgidus oligosaccharyltransferases.2013

    • Author(s)
      Matsumoto S, Shimada A, Kohda D
    • Journal Title

      BMC Struct Biol

      Volume: 13 Issue: 1

    • DOI

      10.1186/1472-6807-13-11

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation.2013

    • Author(s)
      Matsumoto S, Shimada A, Nyirenda J, Igura M, Kawano Y, Kohda D
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 110 Issue: 44 Pages: 17868-17873

    • DOI

      10.1073/pnas.1309777110

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallographic and NMR evidence for flexibility in oligosaccharyltransferases and its catalytic significance2013

    • Author(s)
      James Nyirenda, et al.
    • Journal Title

      Structure

      Volume: 21 Issue: 1 Pages: 32-41

    • DOI

      10.1016/j.str.2012.10.011

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the C-terminal globular domain of oligosaccharyltransferase from Archaeoglobus fulgidus at 1.75Å resolution.2012

    • Author(s)
      Matsumoto, S., Igura, M., Nyirenda, J., Matsumoto. M, Yuzawa, S., Noda, N., Inagaki, F., Kohda, D.
    • Journal Title

      Biochemistry

      Volume: 51 Issue: 20 Pages: 4157-4166

    • DOI

      10.1021/bi300076u

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation2015

    • Author(s)
      Daisuke Kohda
    • Organizer
      Progress 100: Kyushu-U and Stanford-U Joint Research and Education Program, First Symposium
    • Place of Presentation
      福岡
    • Year and Date
      2015-03-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation2014

    • Author(s)
      Daisuke Kohda
    • Organizer
      2014 SFG & JSCR joint annual meeting
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2014-11-16 – 2014-11-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] タンパク質の膜透過と共役したオリゴ糖転移酵素の反応様式の解明2014

    • Author(s)
      山崎貴大, 神田大輔
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 古細菌由来オリゴ糖転移酵素の基質ペプチド複合体の結晶構造2014

    • Author(s)
      松本 俊介, 嶋田 睦, 遠藤 斗志也, 神田 大輔
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Crystal structures of oligosaccharyltransferase provide insights into the sequon recognition and activation of N-linked protein glycosylation2014

    • Author(s)
      Daisuke Kohda
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 超好熱性古細菌由来N結合型オリゴ糖鎖の化学構造決定2014

    • Author(s)
      藤浪大輔,James Nyirenda,神田大輔
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation2014

    • Author(s)
      Shunsuke Matsumoto, Atsushi Shimada, Takahiro Yamasaki, Daisuke Kohda
    • Organizer
      27th International Carbohydrate Symposium
    • Place of Presentation
      Bangalore, India
    • Related Report
      2013 Annual Research Report
  • [Presentation] Crystal structure of an archaeal oligosaccharyltransferase2013

    • Author(s)
      Shunsuke Matsumoto
    • Organizer
      Glycobiology Gordon Research Conference
    • Place of Presentation
      Ventura, CA, USA(ポスターから口頭発表に選出)
    • Year and Date
      2013-03-04
    • Related Report
      2012 Annual Research Report
  • [Presentation] Crystal structure of an archaeal oligosaccharyltransferase, a membrane enzyme that catalyzes the transfer of sugar chains to asparagine residues of glycoproteins2013

    • Author(s)
      Daisuke Kohda
    • Organizer
      Post-GCOE Symposium & Retreat in Singapore with NUS and TLL
    • Place of Presentation
      Singapore
    • Year and Date
      2013-03-04
    • Related Report
      2012 Annual Research Report
  • [Presentation] Structural biology of the N-glycosylation reaction2012

    • Author(s)
      Daisuke Kohda
    • Organizer
      The 20th Anniversary of the Mizutani Foundation for Glycosciences
    • Place of Presentation
      Tokyo, Japan(招待講演)
    • Year and Date
      2012-11-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] 古細菌型オリゴ糖転移酵素によるN型糖鎖修飾の構造基盤の解明

    • Author(s)
      松本 俊介, 嶋田 睦, ジェイムス ニレンダ, 井倉 真由美, 河野 能顕, 神田 大輔
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] 超好熱性古細菌由来N結合型糖鎖の化学構造決定

    • Author(s)
      藤浪大輔,ジェイムス ニレンダ,神田大輔
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] 超好熱性古細菌由来のオリゴ糖転移酵素の基質となるオリゴ糖鎖の化学構造決定と相互作用解析

    • Author(s)
      藤浪大輔,神田大輔
    • Organizer
      第52回NMR討論会
    • Place of Presentation
      金沢
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structural elucidation of N-linked oligosaccharides of hyperthermophilic archaea

    • Author(s)
      Daisuke Fujinami, Masaki Matsumoto, Daisuke Kohda
    • Organizer
      International Symposium between Kyushu U. Post Global COE and School of Biomedical Sciences, Monash U.
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2013 Annual Research Report
  • [Book] Glycoscience: Biology and Medicine (ISBN 978-4-431-54840-9)2014

    • Author(s)
      Shunsuke Matsumoto, James Nyirenda, Daisuke Kohda
    • Total Pages
      1556
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] SPring-8 Research Frontiers 2013 (ISSN 1349-0087)2014

    • Author(s)
      James Nyirenda, Shunsuke Matsumoto, and Daisuke Kohda
    • Total Pages
      153
    • Publisher
      SPRing8
    • Related Report
      2014 Annual Research Report
  • [Remarks] 九州大学生体防御医学研究所構造生物学分野研究内容

    • URL

      http://vsb.bmr.kyushu-u.ac.jp/VSB/Research/research_frame_content_jp.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] MIB VSB Top page

    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.bioreg.kyusyu-u.ac.jp/vsb/index.html

    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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