| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
|
|---|
| Outline of Final Research Achievements |
Pathogenic bacteria such as Shigella, EPEC, and Salmonella deliver a variety of virulence factors, called effectors, into host cells via the type III secretion system. These effectors mimic or hijack host proteins and modulate host signaling pathways to promote bacterial infection. OspI, a Shigella flexneri effector, is a glutamine deamidase that selectively deamidates the glutamine residue at position 100 in Ubc13 to a glutamic acid residue. This modification inhibits the E2 ubiquitin conjugating activity, which is required for TRAF6 activation. To elucidate the molecular mechanisms of OspI, we determined the crystal structures of OspI mutant and its complex with Ubc13. These structures provide the substrate recognition and catalytic mechanism.
|
