Structural analyses of bacterial effectors and their mechanisms of infection
Project/Area Number |
24370049
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | University of Hyogo |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
KIM Minsoo 東京大学, 医科学研究所, 特任准教授 (50466835)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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Keywords | エフェクター / 赤痢菌 / X線結晶構造解析 / ユビキチン経路 / 蛋白質 / 細菌 / エフェクタータンパク質 / 複合体構造 / OspI |
Outline of Final Research Achievements |
Pathogenic bacteria such as Shigella, EPEC, and Salmonella deliver a variety of virulence factors, called effectors, into host cells via the type III secretion system. These effectors mimic or hijack host proteins and modulate host signaling pathways to promote bacterial infection. OspI, a Shigella flexneri effector, is a glutamine deamidase that selectively deamidates the glutamine residue at position 100 in Ubc13 to a glutamic acid residue. This modification inhibits the E2 ubiquitin conjugating activity, which is required for TRAF6 activation. To elucidate the molecular mechanisms of OspI, we determined the crystal structures of OspI mutant and its complex with Ubc13. These structures provide the substrate recognition and catalytic mechanism.
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Report
(4 results)
Research Products
(16 results)
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[Presentation] Structural Basis for the Recognition of Ubc13 by the Shigella flexneri Effector OspI2015
Author(s)
Nishide, A., Kim, M., Takagi, K., Himeno, A., Sanada, T., Sasakawa, C., Mizushima, T.
Organizer
The Annual Evaluation Conference of the Leading Program, University of Hyogo
Place of Presentation
先端科学技術支援センター (兵庫・赤穂郡上郡町)
Year and Date
2015-03-16 – 2015-03-17
Related Report
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[Presentation] Structural Basis for the Recognition of Ubc13 by the Shigella flexneri Effector OspI2014
Author(s)
Nishide, A., Kim, M., Takagi, K., Himeno, A., Sanada, T., Sasakawa, C., Mizushima, T.
Organizer
Symposium for young ubiquitin researchers in Japan “New Era in the Ubiquitin Research
Place of Presentation
国際高等研究所(京都・木津川市)
Year and Date
2014-11-11 – 2014-11-12
Related Report
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