| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Outline of Final Research Achievements |
Nucleosomes are the fundamental repeating units of chromatin. To initiate the transcription reaction, histones must be removed from genomic DNA at gene coding regions. Previously, we determined the cocrystal structure of CIA and double bromodomain, and proposed a recruitment and disassembly model (hiMOST model). In this study, we established the methods for large-scale purification of the HAT domain of TAF1, and the conserved domain of TAF7. In order to perform functional analysis of TFIID in chromatin transcription, we also tried to reconstitute the complex of TBP and TFIIB on promoter DNA.
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