| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
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| Outline of Final Research Achievements |
The purpose of this study is to unravel the regulation mechanisms of signal transduction in lipid rafts of cell plasma membranes. We tracked single molecules of representative raft molecules, GPI-anchored proteins and gangliosides in living cell membranes. Surprisingly, these raft markers formed transient homodimers which were stabilized by cholesterol. In general, the lifetime of the heterodimers were shorter than those of the homodimers. Our results suggest that these homodimers are induced by spesific protein interactions or specific sugar-chain interactions. Based on these results, we proposed a hypothesis that these homodimers are basic units for creating greater raft domains.
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