Dynamic regulation of innate immune responses in Drosophila by Senju-mediated glycosylation.

• Project/Area Number: 24370057
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Rikkyo University
• Principal Investigator: GOTO Satoshi 立教大学, 理学部, 教授 (60280575)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: 自然免疫 / 糖鎖 / ガラクトース / 遺伝学

Outline of Final Research Achievements

The innate immune system is the first line of defense against invading pathogens. Activation of the innate immune response by infection and suppression during steady state are stringently controlled to eliminate pathogens and prevent inflammation. We found that host glycosylation plays an important role in the activation of immune responses and in maintaining innate immune homeostasis. In the steady state, a high amount of galactose-containing glycan suppresses undesirable activation of the immune response; however, activation of immune responses leads to reduced levels of galactose-containing glycan, which is needed for raising immune responses to an adequate level. Our finding suggests a novel mechanism for the regulation of innate immune quiescence and activation via changes in glycosylation status.

Research Products

• [Journal Article] Dynamic regulation of innate immune responses in Drosophila by Senju-mediated glycosylation. (2015)
  • Author(s): Yamamoto-Hino, M., Muraoka, M., Kondo, S., Ueda, R., Okano, H. and Goto, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing. (2015)
  • Author(s): Yamamoto-Hino,M., Yoshida,H., Ichimiya,T., Sakamura,S., Maeda,M., Kimura,Y., Sasaki,N., Aoki-Kinoshita,K.Y, Kinoshita-Toyoda,A., Toyoda,H., Ueda,R., Nishihara,S., and Goto,S.
  • Journal Title: Genes Cells Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Localization of glycosyl enzymes and nucleotide-sugar transporters in the endoplasmic reticulum and the Golgi apparatus. (2014)
  • Author(s): Yamamoto-Hino,M. and Goto,S.
  • Journal Title: Glycoscience: Biology and Medicine Volume: - Pages: 1-5
  • DOI: 10.1007/978-4-431-54836-2_48-1
  • ISBN: 9784431548362
  • Peer Reviewed
• [Journal Article] In vivo RNAi-based screens:studies in model organisms (2013)
  • Author(s): Yamamoto-Hino,M, Goto,S
  • Journal Title: Genes Volume: 4 Issue: 4 Pages: 646-665
  • DOI: 10.3390/genes4040646
  • Peer Reviewed
• [Journal Article] Identification of proteasome components required for apical localization of Chaoptin using functional genomics (2012)
  • Author(s): Yano, H., Yamamoto-Hino, M., Awano, W., Aoki-Kinoshita, K. F., Tsuda-Sakurai, K., Okano, H. and Goto, S.
  • Journal Title: J. Neurogenet Volume: vol.26 Issue: 1 Pages: 53-63
  • DOI: 10.3109/01677063.2012.661497
  • Peer Reviewed
• [Journal Article] Balanced ubiquitination determines cellular responsiveness to extracellular stimuli. (2012)
  • Author(s): Mukai, A.
  • Journal Title: Cell. Mol. Life Sci. Volume: 69 Issue: 23 Pages: 4007-4016
  • DOI: 10.1007/s00018-012-1084-4
  • Peer Reviewed
• [Presentation] 糖鎖修飾の変化による自然免疫反応のダイナミックな制御 (2014)
  • Author(s): 山本（日野）美紀、村岡 正敏、近藤 周、岡野 栄之、上田 龍、後藤 聡
  • Organizer: 第２５回日本生体防御学会
  • Place of Presentation: 東北大学（宮城県仙台市）
  • Year and Date: 2014-07-09 – 2014-07-11
• [Presentation] 糖鎖修飾による自然免疫反応の恒常性維持 (2014)
  • Author(s): 山本（日野）美紀、村岡 正敏、近藤 周、岡野 栄之、上田 龍、後藤 聡
  • Organizer: 第６６回日本細胞生物学会
  • Place of Presentation: 東大寺総合文化センター（奈良県奈良市）
  • Year and Date: 2014-06-11 – 2014-06-13
• [Presentation] Dynamic regulation of innate immue responses by Senju-mediated glycosylation in Drosophila (2014)
  • Author(s): Miki Yamamoto (Hino), Masatoshi Muraoka, Shu Kondo, Hedeyuki Okano, Ryu Ueda, Satoshi Goto
  • Organizer: 第１１回 日本ショウジョウバエ研究会
  • Place of Presentation: 金沢歌劇座（石川県金沢市）
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] 糖鎖修飾による自然免疫反応の恒常性維持 (2014)
  • Author(s): 山本（日野）美紀、村岡 正敏、近藤 周、岡野 栄之、上田 龍、後藤 聡
  • Organizer: 平成２６年度 日本生化学会九州支部例会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2014-05-17 – 2014-05-18
  • Invited
• [Presentation] 1. Dynamic regulation of innate immune responses in Drosophila by Senju-mediated glycosylation (2013)
  • Author(s): Miki Yamamoto-Hino, Masatoshi Muraoka, Hideyuki Okano, Satoshi Goto
  • Organizer: 第３６回日本分子生物学会年会 ワークショップ
  • Place of Presentation: 神戸ポートアイランド（神戸）
• [Presentation] Novel Roles of Glvcosylation in Drosophila Innate Immunity (2012)
  • Organizer: The 12^<th>Biennial International Endotoxin & Innate Immunity Society (IEI1S) meeting
  • Place of Presentation: 東京National Center of Sciences Building
  • Year and Date: 2012-10-24
• [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity (2012)
  • Author(s): Yamamoto-Hino M, et al.
  • Organizer: 第10回日本ショウジョウバエ研究会
  • Place of Presentation: 東京慈恵医大
  • Year and Date: 2012-10-13
• [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity (2012)
  • Author(s): Yamamoto-Hino M, et al.
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-05-31