The elucidation of origin of rotor in rotary ATPase/synthases by functional and structural analysis
| Project/Area Number |
24370059
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
YOKOYAMA Ken 京都産業大学, 総合生命科学部, 教授 (70271377)
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| Co-Investigator(Kenkyū-buntansha) |
IMADA Katsumi 大阪大学, 理学研究科, 教授 (40346143)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Keywords | bioenergetics / V-ATPase / FoF1 / ATP synthase / molecular motor / molecular evolution / flagellar motor / rotary motor / ATPase / 回転分子モーター / べん毛 / F-ATPase / Bioenergetics / Rotary motor / 分子モーター / 分子進化 / 回転 |
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| Outline of Final Research Achievements |
In this study, we revealed that FliJ of bacterial flagella motor is possibly an origin of the rotor in rotary ATPase/synthase such as V- or F-ATPases. Actually, FliJ shows a rotor like function in A3B3 based on both biochemical and structural analysis. This strongly suggests that the rotor domains of the rotary ATPases and the flagellar export apparatus share a common evolutionary origin. Domain-swapped chimeric V1 consisting of both T. thermophilus and E. hirae enzymes were generated. Analysis for these enzymes indicated that the interaction between the nucleotide binding and C-terminal domains of the catalytic A subunit critical in reduction of the ADP inhibition. Based on the reconstituted experiments, we proposed a model in which the relationship between V1-DF and Vo-C corresponds to that between a slotted screwdriver and a head of slotted screw, consistent with our previous result in which the central rotor apparatus is not the major factor for the association of V1 with Vo.
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Report
(4 results)
Research Products
(23 results)
