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Study of Dynamical Aspect of Photosynthetic Proteins Observed with the Newly Developed Cryogenic Microscope

Research Project

Project/Area Number 24370060
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biophysics
Research InstitutionTohoku University

Principal Investigator

SHIBATA Yutaka  東北大学, 理学(系)研究科(研究院), 准教授 (20300832)

Co-Investigator(Kenkyū-buntansha) NOGUCHI Takumi  名古屋大学, 大学院理学研究科, 教授 (60241246)
Research Collaborator NAMIE Keisuke  
CHIBA Tomofumi  
DU Ting  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywords1分子分光 / 顕微蛍光分光 / 極低温 / 光化学系I / クラミドモナス / 顕微分光 / 1分子分光 / エネルギー移動 / FLIM / 2光子励起顕微鏡
Outline of Final Research Achievements

I developed a cryogenic microscope with a high spatial resolution. Using the developed system, I studied the dynamic movements of the antenna proteins, LHCII, during the state transition, which is a typical light-adaptation function of a green alga, chlamydomonas.
I conducted the single-molecule fluorescence spectroscopy for a photosynthetic protein, photosystem I (PS I). I observed for the first time the blinking of fluorescence from single PS I at around 90 K. I considered the imprecation of this observation for the conformation fluctuation of PS I.
I succeeded in reproducing the temperature dependence of the time-resolved fluorescence spectra of photosystem II using a microscopic model calculation based on the structure.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (27 results)

All 2015 2014 2013 2012

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (18 results) (of which Invited: 2 results)

  • [Journal Article] Scrambled Self-Assembly of Bacteriochlorophylls c and e in Aqueous Triton X-100 Micelles2014

    • Author(s)
      Yoshitaka Saga, Tatsuya Saiki, Naoya Takahashi, Yutaka Shibata, and Hitoshi Tamiaki
    • Journal Title

      Photochem. and Photobiol.

      Volume: 90 Issue: 3 Pages: 552-559

    • DOI

      10.1111/php.12219

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a novel cryogenic microscope with numerical aperture of 0.9 and its application to photosynthesis research2014

    • Author(s)
      Y. Shibata, W. Katoh, T. Chiba, K. Namie, N. Ohnishi, J. Minagawa, H. Nakanishi, T. Noguchi and H. Fukumura
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1837 Issue: 6 Pages: 880-887

    • DOI

      10.1016/j.bbabio.2014.03.006

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 光合成系の光捕集過程を構造に立脚して理解する:理論と実験の融合で見えてきたこと2014

    • Author(s)
      柴田 穣
    • Journal Title

      日本物理学会誌

      Volume: 69 Pages: 639-643

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 開口数0.9を持つ極低温顕微鏡の開発2014

    • Author(s)
      柴田穣,加藤渉,浪江慶祐
    • Journal Title

      分光研究

      Volume: 63 Pages: 103-105

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Study of Cell-Differentiation and Assembly of Photosynthetic Proteins during Greening of Etiolated Zea mays Leaves Using Confocal Fluorescence Microspectroscopy at Liquid-Nitrogen Temperature2013

    • Author(s)
      Yutaka Shibata, Wataru Kato, and Yukari Tahara
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1827 Issue: 4 Pages: 520-528

    • DOI

      10.1016/j.bbabio.2013.02.001

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two types of fucoxanthin-chlorophyll-binding proteins I tightly bound to the photosystem I core complex in marine centric diatoms2013

    • Author(s)
      Yohei Ikeda, Atsushi Yamagishi, Masayuki Komura, Takehiro Suzuki, Naoshi Dohmae, Yutaka Shibata, Shigeru Itoh, Hiroyuki Koike, Kazuhiko Satoh
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: 1827 Issue: 4 Pages: 529-539

    • DOI

      10.1016/j.bbabio.2013.02.003

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photosystem II does not possess a simple excitation energy funnel: Time-resolved fluorescence spectroscopy meets theory2013

    • Author(s)
      Yutaka Shibata, Shunsuke Nishi, Keisuke Kawakami, Jian-Ren Shen, Thomas Renger
    • Journal Title

      J. Am. Chem. Soc.

      Volume: - Issue: 18 Pages: 6903-6914

    • DOI

      10.1021/ja312586p

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabitol Provided by Lichenous Fungi Enhances Ability to Dissipate Excess Light Energy in a Symbiotic Green Alga under Desiccation2013

    • Author(s)
      Makiko Kosugi, Hirohisa Miyake, Hisanori Yamakawa, Yutaka Shibata, Atsuo Miyazawa, Takashi Sugimura, Kazuhiko Satoh, Shigeru Itoh, Yasuhiro Kashino
    • Journal Title

      Plant and Cell Physiology

      Volume: 54 Issue: 8 Pages: 1316-1325

    • DOI

      10.1093/pcp/pct079

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Supramolecular energy transfer from photoexcited chlorosomal zinc porphyrin self-aggregates to a chlorin or bacteriochlorin monomer as models of main light-harvesting antenna systems in green photosynthetic bacteria2012

    • Author(s)
      Yumiko Kataoka, Yutaka Shibata, and Hitoshi Tamiaki
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 22

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 緑色イオウ光合成細菌Chlorobaculum limnaeum RK-j-1株の変異体から単離精製した色素の異なるクロロソームの比較評価2015

    • Author(s)
      原田 二朗、柴田 穣、溝口 正、漁野 岬、寺村 美里、山本 健、民秋 均
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      日本大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ピコ秒時間分解蛍光分光による極地に生育する蘚類および藻類の乾燥耐性の分子機構の研究2015

    • Author(s)
      柴田 穣、谷山 航一朗、Ahmed Ali、小杉 真貴子、福村 裕史
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] CHARACTERIZATION OF CHLOROSOMES VARIANTS CONTAINING BACTERIOCHLOROPHYLL c, d, e, OR f IN THE MUTANTS DERIVED FROM THE IDENTICAL STRAIN OF BROWN-COLORED GREEN SULFUR BACTERIUM2014

    • Author(s)
      Jiro Harada, Yutaka Shibata, Misaki Ryono, Ken Yamamoto, Tadashi Mizoguchi, Hitoshi Tamiaki
    • Organizer
      2014 International Conference on Artificial Photosynthesis
    • Place of Presentation
      Awaji City, Hyogo, Japan
    • Year and Date
      2014-11-24 – 2014-11-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 極低温顕微鏡を用いた緑化途上トウモロコシ生葉の光合成タンパク質前駆体の空間分布の測定2014

    • Author(s)
      千葉 知史、福村 裕史、柴田 穣
    • Organizer
      日本生物物理学会第52回年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 光合成蛋白質と金属ナノ粒子による水素発生人工光合成ナノデバイスの開発2014

    • Author(s)
      田原 一輝、河原 弘典、浪江 慶祐、井上 名津子、長尾 遼、加藤 祐樹、鞆 達也、柴田 穣、福村 裕史、菓子野 康浩、野口 巧
    • Organizer
      日本生物物理学会第52回年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Target analysis of the photosystem II-enriched membrane: The effect of oxidizing agent on the fluorescence quenching in PSII2014

    • Author(s)
      Ahmed Ali, Ryo Nagao, Takumi Noguchi, Hiroshi Fukumura, Yutaka Shibata
    • Organizer
      日本生物物理学会第52回年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 開口数0.9の極低温顕微鏡の開発と光合成タンパク質の1分子分光への応用2014

    • Author(s)
      柴田 穣
    • Organizer
      日本分析化学会
    • Place of Presentation
      広島大学
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 新開発の開口数0.9の極低温光学顕微鏡による細胞内光合成タンパク質の不均一分布測定2014

    • Author(s)
      柴田 穣、千葉 知史、福村 裕史
    • Organizer
      第36回日本光医学・光生物学会年会
    • Place of Presentation
      大阪大学
    • Year and Date
      2014-07-25 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 理論と実験の融合で見えてきた植物型光合成の超高速光反応2014

    • Author(s)
      柴田 穣
    • Organizer
      第11回AMO討論会
    • Place of Presentation
      大阪大学
    • Year and Date
      2014-06-06 – 2014-06-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 光合成タンパク質と金属ナノ粒子による水素発生人工光合成ナノデバイスの開発2014

    • Author(s)
      田原 一輝、河原 弘典、浪江 慶祐、井上 名津子、長尾 遼、加藤 祐樹、鞆 達也、柴田 穣、福村 裕史、菓子野 康浩、野口 巧
    • Organizer
      第5回光合成学会年会
    • Place of Presentation
      近畿大学
    • Year and Date
      2014-05-30 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新しく開発した極低温顕微鏡による光化学系Iの1分子蛍光分光2014

    • Author(s)
      浪江 慶祐、加藤 渉、中西 華代、野口 巧、福村 裕史、柴田 穣
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Dedicated comparison of light-harvesting dynamics between the cyanobacterial and the higher plant photosystem II2014

    • Author(s)
      Ahmed Ali、柴田 穣、長尾 遼、野口 巧、福村 裕史
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物型光合成反応中心の光捕集過程:構造情報に基づく理解22013

    • Author(s)
      架田穣、西俊輔、川上恵典、沈建仁、Thomas Renger
    • Organizer
      日本物理学会2013年年会
    • Place of Presentation
      広島大学
    • Year and Date
      2013-03-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] Application of the home-built cryogenic microspectroscopy system with numerical aperture of 0.9 to photosynthetic organisms2013

    • Author(s)
      Keisuke Namie, Tomofumi Chiba, Mizu Kajihara, Wataru Kato, Hiroshi Fukumura, Yutaka Shibata
    • Organizer
      16th International Photosynthesis Congress
    • Place of Presentation
      Saint Louis, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Light-harvesting dynamics in photosystem II: a combination of time-resolved fluorescence spectroscopy and microscopic theory2013

    • Author(s)
      Yutaka Shibata, Ahmed Ibrahim Ali Mohamed, Shunsuke Nishi, Keisuke Kawakami, Jian-Ren Shen, Ryo Nagao, Takumi Noguchi, Hiroshi Fukumura, Thomas Renger
    • Organizer
      16th International Photosynthesis Congress
    • Place of Presentation
      Saint Louis, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 柴田 穣、浪江 慶祐、千葉 知史、梶原 瑞、加藤 渉、福村 裕史、開口数0.9の極低温光学顕微鏡の開発とその植物細胞内色素イメージングへの応用2013

    • Author(s)
      柴田 穣、浪江 慶祐、千葉 知史、梶原 瑞、加藤 渉、福村 裕史
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of cryogenic microspectroscopy system with numerical aperture of 0.9 applicable to biological systems2012

    • Author(s)
      Keisuke Namie, Wataru Kato, Hiroshi Fukumura, Yutaka Shibata
    • Organizer
      7th Asian Photochemistry Conference
    • Place of Presentation
      大阪大学
    • Year and Date
      2012-11-12
    • Related Report
      2012 Annual Research Report
  • [Presentation] Possible non-photochemical quenching mechanism within photosystem II core complex2012

    • Author(s)
      柴田穣、酉俊鞆、川上恵典、沈建仁、Thomas Renger
    • Organizer
      日本生物物理学会第50回年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-09-24
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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