Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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Outline of Final Research Achievements |
I developed a cryogenic microscope with a high spatial resolution. Using the developed system, I studied the dynamic movements of the antenna proteins, LHCII, during the state transition, which is a typical light-adaptation function of a green alga, chlamydomonas. I conducted the single-molecule fluorescence spectroscopy for a photosynthetic protein, photosystem I (PS I). I observed for the first time the blinking of fluorescence from single PS I at around 90 K. I considered the imprecation of this observation for the conformation fluctuation of PS I. I succeeded in reproducing the temperature dependence of the time-resolved fluorescence spectra of photosystem II using a microscopic model calculation based on the structure.
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