Study of Dynamical Aspect of Photosynthetic Proteins Observed with the Newly Developed Cryogenic Microscope
Project/Area Number |
24370060
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Biophysics
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Research Institution | Tohoku University |
Principal Investigator |
SHIBATA Yutaka 東北大学, 理学(系)研究科(研究院), 准教授 (20300832)
|
Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Takumi 名古屋大学, 大学院理学研究科, 教授 (60241246)
|
Research Collaborator |
NAMIE Keisuke
CHIBA Tomofumi
DU Ting
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
|
Keywords | 1分子分光 / 顕微蛍光分光 / 極低温 / 光化学系I / クラミドモナス / 顕微分光 / 1分子分光 / エネルギー移動 / FLIM / 2光子励起顕微鏡 |
Outline of Final Research Achievements |
I developed a cryogenic microscope with a high spatial resolution. Using the developed system, I studied the dynamic movements of the antenna proteins, LHCII, during the state transition, which is a typical light-adaptation function of a green alga, chlamydomonas. I conducted the single-molecule fluorescence spectroscopy for a photosynthetic protein, photosystem I (PS I). I observed for the first time the blinking of fluorescence from single PS I at around 90 K. I considered the imprecation of this observation for the conformation fluctuation of PS I. I succeeded in reproducing the temperature dependence of the time-resolved fluorescence spectra of photosystem II using a microscopic model calculation based on the structure.
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Report
(4 results)
Research Products
(27 results)
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[Presentation] CHARACTERIZATION OF CHLOROSOMES VARIANTS CONTAINING BACTERIOCHLOROPHYLL c, d, e, OR f IN THE MUTANTS DERIVED FROM THE IDENTICAL STRAIN OF BROWN-COLORED GREEN SULFUR BACTERIUM2014
Author(s)
Jiro Harada, Yutaka Shibata, Misaki Ryono, Ken Yamamoto, Tadashi Mizoguchi, Hitoshi Tamiaki
Organizer
2014 International Conference on Artificial Photosynthesis
Place of Presentation
Awaji City, Hyogo, Japan
Year and Date
2014-11-24 – 2014-11-28
Related Report
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