Elucidation of biological functions of LEA proteins as a desiccation protectant and their industrial application
| Project/Area Number |
24370065
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
SAKURAI Minoru 東京工業大学, バイオ研究基盤支援総合センター, 教授 (50162342)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KIKAWADA Takahiro 独立行政法人農業生物資源研究所, 乾燥耐性研究ユニット, 主任研究員 (60414900)
SHIRAKASHI Ryo 東京大学, 生産技術研究所, 教授 (80292754)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
|
|---|
| Keywords | LEAタンパク質 / LEAペプチド / 乾燥耐性 / リポソームの融合 / タンパク質の凝集 / MDシミュレーション / アミロイド凝集 / ペプチド / リン脂質二重膜 / ラクトースデヒドロゲナーゼ / タンパク質凝集 / 水 / リポソーム / トレハロース |
|---|
| Outline of Final Research Achievements |
We investigated biological functions of a LEA peptide that has two tandem repeat of the 11-mer motif characteristic of late embryogenesis abundant protein found in anhydrobitic organisms. It was shown that this peptide acts well as a desiccation protectant: it can suppress desiccation-induced protein aggregation and liposome fusion.
|
Report
(4 results)
Research Products
(23 results)
-
-
-
-
[Journal Article] An abundant LEA protein in the anhydrobiotic midge, PvLEA4, acts as a molecular shield by limiting growth of aggregating protein particles2013
Author(s)
R. Hatanaka, Y. Hagiwara-Komoda, T. Furuki, Y. Kanamori, M. Fujita, R. Cornette, M. Sakurai, T. Okuda, T. Kikawada
-
Journal Title
Insect Biochem. Mol. Biol.
Volume: 43
Pages: 1055-1067
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Effects of Group 3 LEA protein model peptides on desiccation-induced protein aggregation2012
Author(s)
Furuki T, Shimizu T, Chakrabortee S, Yamakawa K, Hatanaka R, Takahashi T, Kikawada T, Okuda T, Mihara H, Tunnacliffe A, Sakurai M
-
Journal Title
Biochimica et Biophysica Acta-Proteins & Proteomics
Volume: 1824
Issue: 7
Pages: 891-897
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
