| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
|
|---|
| Outline of Final Research Achievements |
① Archaerhodopsin-2 was crystallized and its structure was determined at 1.8 Åresolution. ② Cruxrhodopsin-3 was crystallized and its structure was determined at 2.1 Å resolution. ③ Deltarhodopsin-3 was crystallized and its trimeric structure was determined at 2.7 Å resolution. ④ The light-driven chloride ion pump halorhodopsin (pHR) from Natronomonas pharonis was crystallized, and the major photoreaction states (L1, L2, N, N’and O states) of pHR-bromide ion complex were determined at 2.2 -2.7 Å resolutions. ⑤ The crystal structure of an M intermediate of pHR-azide complex was determined at 2.3 Å resolution. ⑥ Various mutants of phoborhodopsin from Halobacterium salinarum were prepared and their photoreaction kinetics were investigated. ⑦ The crystal structure of the Lumi state of squid rhodopsin was determined at 2.7 Å resolution. ⑧ Octopus rhodopsin was crystallized by the membrane fusion method.
|
