| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
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| Outline of Final Research Achievements |
To understand various biological functions, observation of the nanometre structures of bacteria and viruses is essential. Scanning electron microscopy (SEM) is an important tool for such observations. However, samples of SEM are usually prepared by staining to enhance image contrast. Staining preparation is often not recommended to observe detail of the biological structures. Moreover, biological samples are prone to radiation damage and unstained samples give very poor contrast. Here, we report new methods for the unstained biological specimens in water of bacteria and virus, proteins. The obtained images show very high-contrast, and the samples incur low-electron radiation damage. Our method can be used for diverse liquid specimens across a broad range, such as nanoparticles and catalytic materials.
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