| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
We have studied on the ribosomal stalk protein complex, which determines the rate and efficiency of protein synthesis (translation), by using biochemical and crystallographic approaches, as follows: 1) multiple copies of the stalk proteins all bind to the ribosomal core via their N-terminal domains, and the C-terminal region of each stalk is flexible and moving widely around the ribosome; 2) the crystal structural data indicate that the C-terminal region of the stalk protein binds to individual translation factors through hydrophobic interactions; 3) the interactions between the C-terminal region of the stalk protein and translation factors is important to recruit the factors to the functional center within the ribosomal core. These lines of evidence demonstrate the crucial contribution of the stalk protein to efficient translation.
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