| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
It has recently been reported that several Rab GTPase-activating proteins (GAPs) localize autophagosomes, but their precise mechanisms of autophagosomal targeting and their functions in autophagy are poorly understood. In this study, we analyzed the LC3 recognition sequence of OATL1, a Rab33B-GAP, and its localization on autopagosomes. We found that OATL1 is specifically localized outside the autophagosomal membrane through interaction with LC3 and that such asymmetric localization enables OATL1 to regulate the autophagosomal maturation without OTAL1 being degraded by autophagy.
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