| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
Centrioles are organelles with a cylindrical shape consisting of nine triplet microtubules. This 9-fold symmetrical structure is highly conserved among centrioles of most eukaryotic organisms. We previously reported that the cartwheel, a subcentriolar structure with 9-fold symmetry, largely contributes to establishment of the 9-fold symmetry of the centriole. In this study, we produced a cartwheel protein that assembles in vitro into cartwheel-like structures with 6-fold symmetry, and expressed it in Chlamydomonas cells. Observations of the centrioles and cartwheels in the cells suggested that a cartwheel-independent mechanism exists in the circular arrangement of the centriolar microtubules, and that a dynamic interaction between the mechanism and the cartwheel precisely determines the number of the triplets in the centriole.
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