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Evocation of the ER stress response by lipid aberrancy

Research Project

Project/Area Number 24370081
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Cell biology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

KIMATA Yukio  奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (60263448)

Co-Investigator(Kenkyū-buntansha) 斉藤 美知子 (斉藤 美智子)  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40379558)
柳谷 耕太  奈良先端科学技術大学院大学, バイオサイエンス研究科, 特任助教 (70614775)
Co-Investigator(Renkei-kenkyūsha) KOHNO Kenji  奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (50142005)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords小胞体 / オルガネラ / 酵母 / ストレス応答 / ストレス / 膜脂質 / 微生物 / 出芽酵母
Outline of Final Research Achievements

The endoplasmic reticulum (ER) is a cellular compartment in which secretory proteins are folded and membrane lipids are metabolized. ER stress, namely dysfunction of the ER, has been believed to be caused by impaired protein folding in the ER, which leads to formation of toxic aggregates of denatured proteins. ER stress activates the ER-located transmembrane protein Ire1, which evokes the unfolded protein response (UPR) to protect cells against ER stress. In the present study, we addressed how membrane-lipid aberrancy activates Ire1 and evokes the UPR. In budding yeast cells, mutant Ire1 which cannot recognize unfolded proteins was normally activated by disturbance of membrane-lipid homeostasis. Also through analysis of proteins which are associated with Ire1 in yeast cells, we could establish a molecular model which explains activation of Ire1 by membrane-lipid aberrancy.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (15 results)

All 2015 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (10 results) (of which Invited: 1 results)

  • [Journal Article] Tight regulation of the unfolded protein sensor Ire1 by its intramolecularly antagonizing subdomain2015

    • Author(s)
      Mathuranyanon R, Tsukamoto T, Takeuchi A, Ishiwata-Kimata Y, Tuchiya Y, Kohno K, Kimata Y.
    • Journal Title

      J. Cell Sci.

      Volume: in press

    • NAID

      120006537402

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Ethanol stress impairs protein folding in the endoplasmic reticulum and activates Ire1 in Saccharomyces cerevisiae2014

    • Author(s)
      Miyagawa K, Ishiwata-Kimata Y, Kohno K, Kimata Y.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 78 Issue: 8 Pages: 1389-1391

    • DOI

      10.1080/09168451.2014.921561

    • NAID

      120006537404

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Zinc depletion activates the endoplasmic reticulum-stress sensor Ire1 via pleiotropic mechanisms.2013

    • Author(s)
      Nguyen TS, Kohno K, Kimata Y.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 77(6) Pages: 1337-9

    • NAID

      10031184709

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] F-actin and a type-II myosin are required for efficient clustering of the ER stress sensor Ire1.2013

    • Author(s)
      Ishiwata-Kimata Y, Yamamoto YH, Takizawa K, Kohno K, Kimata Y.
    • Journal Title

      Cell Struct Funct.

      Volume: 38(2) Pages: 135-43

    • NAID

      130004053895

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A BiP-bound and non-clustered mode of Irel evokes a weak but sustained unfolded protein response.2013

    • Author(s)
      Ishiwata-Kimata, Yuki
    • Journal Title

      Genes Ceils

      Volume: 18 Issue: 4 Pages: 288-301

    • DOI

      10.1111/gtc.12035

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Fine regulation of the endoplasmic reticulum-stress sensor Ire1 under various cellular situations2014

    • Author(s)
      Yukio Kimata, Kenji Kohno
    • Organizer
      日本生化学会
    • Place of Presentation
      京都国際会館(京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ミトコンドリアの機能不全はUnfolded protein responseにどのように関わるのか?2014

    • Author(s)
      木俣有紀、小口能里枝、木俣行雄
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2014-09-01 – 2014-09-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] 出芽酵母における小胞体ストレスセンサーIre1の機能と制御2014

    • Author(s)
      木俣有紀、河野憲二、木俣行雄
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      奈良県新公会堂(奈良市)
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] The distinct roles of IRE1 paralogues in mammalian secretory cells2013

    • Author(s)
      Rubwad Mathuranyanon, Kenji Kohno, Yukio Kimata
    • Organizer
      FASEB “ From Unfolded Proteins in the ER to Disease”
    • Place of Presentation
      Vermont Academy Saxtons River, VT, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Regulation of the ER stress sensor Ire1 by its N-terminal intrinsically disordered region2013

    • Author(s)
      Rubwad Mathuranyanon, Kenji Kohno, Yukio Kimata
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      ウインクあいち、愛知県名古屋市
    • Related Report
      2013 Annual Research Report
  • [Presentation] The ER stress sensor Ire1 phosphorylates and stabilizes the ion transporter Zrg17 to enable proper zinc distribution in yeast cells.2013

    • Author(s)
      Yukio Kimata
    • Organizer
      Gordon Research Conference ”Stress Proteins in Growth, Development & Disease”
    • Place of Presentation
      Mount Snow Resort, VT, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 小胞体ストレスセンサーIre1の活性調節とストレス感知:サイトゾル側ドメインの関与2013

    • Author(s)
      小口能里枝、木俣有紀、河野憲二、木俣行雄
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド、兵庫県神戸市
    • Related Report
      2013 Annual Research Report
  • [Presentation] N末端天然非変性領域による小胞体ストレスセンサーIre1の厳密な制御2013

    • Author(s)
      塚本知子、Mathuranyanon Rubwad、河野憲二、木俣行雄
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド、兵庫県神戸市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 出芽酵母でのエタノール誘発性小胞体ストレスと防衛応答2013

    • Author(s)
      宮川賢一、河野憲二、木俣行雄
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド、兵庫県神戸市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Membrane aberrancy and unfolded proteins activate the endoplasmic reticulum-stress sensor Irel by different manners.2012

    • Author(s)
      Yukio Kimata
    • Organizer
      日本細胞生物学会シンポジウム講演(招待)
    • Place of Presentation
      神戸国際会議場(兵庫県)(招待講演)
    • Year and Date
      2012-05-30
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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