Study of the roles of Ser/Thr-Pro consensus motifs for Cdk1 in mitotic regulation
| Project/Area Number |
24370083
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Kyushu University |
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Principal Investigator |
SAGATA Noriyuki 九州大学, 理学(系)研究科(研究院), 教授 (80142024)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAJO Nobushige 九州大学, 理学研究院, 講師 (90294876)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | 細胞周期 / M期 / Cdk1キナーゼ / リン酸化モチーフ / ツメガエル卵 / HeLa細胞 |
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| Outline of Final Research Achievements |
In this study, first we identified the consensus sequence of the novel phosphorylation motif (non-S/TP motif) of Cdk1 and clarified the relationship between Cdk1 phosphorylations of the non-S/TP motif and the classical S/TP motif. Furthermore, we showed the binding of multiple M phase kinases to the S/TP motif and also the mode of the bindings. Moreover, we demonstrated the concerted regulation of the M phase by binding of the multiple M phase kinases to the S/TP motif. Thus, our study showed the molecular basis of the mechanism by which Cdk1 functions as the master M phase kinase.
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Report
(4 results)
Research Products
(15 results)
