Deciphering the function for S1P transporter, Spns2, in mammals
| Project/Area Number |
24370084
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
MOCHIZUKI Naoki 独立行政法人国立循環器病研究センター, 研究所, 部長 (30311426)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Hiroyuki 国立循環器病研究センター, 研究所, 研究員 (10467657)
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| Co-Investigator(Renkei-kenkyūsha) |
FUKUHARA Shigetomo 国立循環器病研究センター, 研究所, 室長 (70332880)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | スフィンゴシン１燐酸 / ノックアウトマウス / 輸送体 / リンパ球 / 胸腺 / 骨髄 / S1P / 眼瞼 / 蛋白質 / 遺伝子 / 動物 / トランスポーター / スフィンゴ脂質 |
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| Outline of Final Research Achievements |
We had identified Spns2 as a S1P transporter in zebrafish. Therefore, we aimed at studying the function of Spns2 in mammals in S1P signaling.
First, we developed global Spns2 knockout (KO) mice and analyzed the number of lymphocytes in primary and secondary lymphatic organs, because S1P is known to be essential for egress from the primary lymphatic organs. Mature T- and B- lymphocytes in the blood were decreased. Mature T-lymphocytes were accumulated in the thymus. Consistently, the number of mature T-lymphocytes was decreased in the secondary lymphatic organs. Similarly, the number of mature B-lymphocytes in the bone marrow and the secondary lymphatic organs was decreased, suggesting that the egress of T- and B-lymphocytes were decreased. The endothelium-specific Spns2 KO mice exhibited the similar phenotype found in global KO mice. These data indicate that endothelial Spns2 functions as a S1P transporter to induce the egress of lymphocytes from the primary lumphatic organs.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Vascular Endothelial Adrenomedullin-RAMP2 System 18 Essential for Vascular Integrity and Organ Homeostasis2013
Author(s)
Teruhide Koyama. Laura Ochoa-Callejero, Takayuki Sakurai, Akiko Kamiyoshi, Yuka Ichikawa-Shindo, Nobuyoshi Iinuma, Takuma Arai, Takahiro Yoshizawa, Yasuhiro Iesato, Yang Lei, Ryuichi Uetake, Akihiro Yamauchi, Megumu Tanaka, Kyoko Igarashi, Yuichi Tbriyama, Ralf H. Adams, Hayato Kawakami, Naoki Mochizuki, Alfredo Martinez, Takayuki Shindo
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Journal Title
Circulation
Volume: 127
Issue: 7
Pages: 842-853
DOI
Related Report
Peer Reviewed
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