Axon formation as a model of structure-function relationship

• Project/Area Number: 24370086
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: TABATA TETSUYA 東京大学, 分子細胞生物学研究所, 教授 (10183865)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: ショウジョウバエ / Sickie / アクチン / Cofilin / slingsohot / Rac / キノコ体 / 軸索伸長 / slingshot / Slingshot

Outline of Final Research Achievements

The Drosophila mushroom body (MB) has been well studied as a model of neuronal development and function. We identified two novel genes, sickie and Disco interacting protein 2 (DIP2) that are required for MB development.
(1) Sickie regulates F-actin-mediated axonal growth via the non-canonical Rac-Cofilin pathway in a Slingshot (Ssh) -dependent manner. Cofilin plays an essential role as a regulator of axonal growth by severing and depolymerizing F-actin. P-Cofilin levels were increased in the pupal brain of sickie mutants.
(2) DIP2 may catalyse the binding of ATP to an as-yet-unidentified substrate with its ATP-synthase domain in theα/βKenyon cells. We propose that DIP2 is regulated by JNK and regulates axon growth and axon branch formation by regulating　planar cell polarity in the growth cone. As candidates of its downstream targets, we identified graikit and crumbs genes.

Research Products

• [Journal Article] The NAV2 homolog Sickie regulates F-actin-mediated axonal growth in Drosophila mushroom body neurons via the non-canonical Rac-Cofilin pathway (2014)
  • Author(s): Abe, T., Yamazaki, D., Murakami, S., Hiroi, M., Nitta, Y., Maeyama, Y., and Tabata T.
  • Journal Title: Development Volume: 141 Issue: 24 Pages: 4716-4728
  • DOI: 10.1242/dev.113308
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] The NAV2 homolog Sickie regulates F-actin-mediated axonal growth in Drosophila mushroom body neurons via the non-canonical Rac-Cofilin pathway (2015)
  • Author(s): Takashi Abe, Daisuke Yamazaki, Satoshi Murakami, Makoto Hiroi, Yohei Nitta, Yuko Maeyama, Tetsuya Tabata
  • Organizer: 2015 Annual Drosophila Research Conference
  • Place of Presentation: シカゴ (アメリカ)
  • Year and Date: 2015-03-04 – 2015-03-08
• [Presentation] Sickie, a human MAP Nav2 homolog, facilitates F-actin-mediated axonal growth in Drosophila MB neurons by relaying non-canonical Rac signaling to the Cofilin pathway (2014)
  • Author(s): 阿部崇志、山崎大介、村上智史、廣井誠、新田陽平、前山有子、多羽田哲也
  • Organizer: 第11回日本ショウジョウバエ研究会
  • Place of Presentation: 金沢歌劇座 (石川県金沢市)
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Disco-interacting protein 2 (DIP2) is required for both proper projection and number of axonal branches in mushroom body (2014)
  • Author(s): 新田陽平、清水一道、大坪真樹、西賢二、程久美子、多羽田哲也
  • Organizer: 第11回日本ショウジョウバエ研究会
  • Place of Presentation: 金沢歌劇座 (石川県金沢市)
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Visualizing Drosophila olfactory memory. (2013)
  • Author(s): Tetsuya Tabata
  • Organizer: ICOB Seminar
  • Place of Presentation: Institute of Cellular and Organismic Biology Arademia Sinica (Taiwan, R.O.C.)(招待講演)
  • Year and Date: 2013-03-25
• [Presentation] Drosophila olfactory coding and memory. (2013)
  • Author(s): Tetsuya Tabata
  • Organizer: Sensory Systems & Neural Circuits
  • Place of Presentation: 伊藤国際学術研究センター(東京都)(招待講演)
  • Year and Date: 2013-02-12
• [Presentation] Disco-interacting protein 2 (DIP2 ) regulates guidance of sister axons in the Drosophila mushroom body (2013)
  • Author(s): Yohei Nitta
  • Organizer: 東京大学生命科学シンポジウム
  • Place of Presentation: 伊藤国際学術研究センター(東京都)
• [Presentation] Sickie, a human MAP Nav2 homolog, regulates F-actin-mediated axonal growth in Drosophila MB neurons via non-canonical Rac/Cofilin pathway (2013)
  • Author(s): Takashi Abe
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館(京都府)
• [Presentation] Tails of the mushroom, their development and function. (2012)
  • Author(s): Tetsuya Tabata
  • Organizer: Swiss Japanese Developmental Biology Meeting
  • Place of Presentation: 京都ガーデンパレス(京都府)(招待講演)
  • Year and Date: 2012-11-05
• [Remarks] 多羽田哲也研究室ホームページ
  • URL: http://www.iam.u-tokyo.ac.jp/fly/htmls/index.htm
• [Remarks] 多羽田哲也研究室
  • URL: http://www.iam.u-tokyo.ac.jp/fly/htmls/index.htm
• [Remarks]
  • URL: http://www.u-tokyo.ac.jp/fly/htmls/index.htm