| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
The Drosophila mushroom body (MB) has been well studied as a model of neuronal development and function. We identified two novel genes, sickie and Disco interacting protein 2 (DIP2) that are required for MB development.
(1) Sickie regulates F-actin-mediated axonal growth via the non-canonical Rac-Cofilin pathway in a Slingshot (Ssh) -dependent manner. Cofilin plays an essential role as a regulator of axonal growth by severing and depolymerizing F-actin. P-Cofilin levels were increased in the pupal brain of sickie mutants.
(2) DIP2 may catalyse the binding of ATP to an as-yet-unidentified substrate with its ATP-synthase domain in theα/βKenyon cells. We propose that DIP2 is regulated by JNK and regulates axon growth and axon branch formation by regulating planar cell polarity in the growth cone. As candidates of its downstream targets, we identified graikit and crumbs genes.
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