Regulatory network and chromatin dynamics of meiosis induction in the germline

• Project/Area Number: 24370089
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: CHUMA SHINICHIRO 京都大学, 再生医科学研究所, 准教授 (20378889)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 減数分裂 / クロマチン / 生殖細胞 / 生殖 / ゲノム / エピゲノム / 発生 / シグナル伝達 / 組み換え

Outline of Final Research Achievements

We established a culture system to induce meiotic transition (leptotene-zygotene stage of prophase I) of mitotically proliferating mouse germline stem cells in vitro. In this research project, we carried out multi-omics analyses and cell biological studies of the in vitro differentiation model of meiosis induction in germline stem cells. Integrated analyses of transcriptome and proteome regulation led to a comprehensive characterization of the key initial processes of chromatin remodeling in meiotic nuclei of mammalian germ cells.

Research Products

• [Journal Article] HSP90α plays an important role in piRNA biogenesis and retrotransposon repression in mouse (2014)
  • Author(s): Ichiyanagi, T., Ichiyanagi, K., Ogawa, A., Kuramochi-Miyagawa, S., Nakano, T., Chuma, S., Sasaki, H.
  • Journal Title: Nucleic Acids Res Volume: 42 Issue: 19 Pages: 11903-11
  • DOI: 10.1093/nar/gku881
  • Peer Reviewed / Open Access
• [Journal Article] LINE-1 of evidence for fetal oocyte attrition by retrotransposon (2014)
  • Author(s): Chuma, S
  • Journal Title: Dev Cell Volume: 29 Issue: 5 Pages: 501-2
  • DOI: 10.1016/j.devcel.2014.05.017
  • Peer Reviewed
• [Journal Article] 減数分裂の発生制御とゲノム情報の継承 (2014)
  • Author(s): 中馬新一郎
  • Journal Title: 実験医学 Volume: 32 Pages: 853-858
• [Journal Article] メタボローム解析によるヒト多能性幹細胞の品質評価技術の開発 (2014)
  • Author(s): 鈴木 崇，末盛 博文，中馬 新一郎，饗庭 一博，中辻 憲夫
  • Journal Title: 島津評論 Volume: in press
  • NAID: 40020064540
• [Journal Article] Tudor domain containing 12 (TDRD12) is essential for secondary PIWI interacting RNA biogenesis in mice (2013)
  • Author(s): Pandey RR, Tokuzawa Y, Yang Z, Hayashi E, Ichisaka T, Kajita S, Asano Y, Kunieda T, Sachidanandam R, Chuma S, Yamanaka S, Pillai RS
  • Journal Title: Proc Natl Acad Sci U S A Volume: 110 Pages: 16492-7
  • Peer Reviewed
• [Journal Article] The nuage mediates retrotransposon silencing in mouse primordial ovarian follicles (2013)
  • Author(s): Lim AK, Lorthongpanich C, Chew TG, Tan CW, Shue YT, Balu S, Gounko N, Kuramochi-Miyagawa S, Matzuk MM, Chuma S, Messerschmidt DM, Solter D, Knowles BB
  • Journal Title: Development Volume: 140 Pages: 3819-25
  • Peer Reviewed
• [Journal Article] GPAT2, a Mitochondrial Acyltransferase, in piRNA Biogenesis in Germline Stem Cells (2013)
  • Author(s): Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Katsumata A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
  • Journal Title: RNA Volume: 19 Pages: 803-10
  • Peer Reviewed
• [Journal Article] piRNA and spermatogenesis in mice (2013)
  • Author(s): Chuma S, Nakano, T
  • Journal Title: Philos Trans R Soc Lond B Biol Sci Volume: (in press)
  • Peer Reviewed
• [Journal Article] GPAT2, a Mitochondrial Acyltransferase, in piRNA Biogenesis in Germline Stem Cells (2013)
  • Author(s): Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Churaa S, Katanaya A, Katsuroata A, Nishimura K, Ohtaka M, Nakanishi M, Nakaraura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojiroa-Kita K, Itou D, Kiraura T and Nakano T
  • Journal Title: RNA Volume: (in press)
  • Peer Reviewed
• [Journal Article] A Role for Fkbp6 and the Chaperone Machinery in piRNA Amplification and Transposon Silencing (2012)
  • Author(s): xiol J, Cora E, Koglgruber R, Chuma S, Subramanian S, Hosokawa M, Reuter M, Yang Z, Berninger P, Palencia A, Benes v, Penninger J, Sachidanandain R, Pillai RS
  • Journal Title: Mol Cell Volume: 47 Pages: 970-979
  • Peer Reviewed
• [Presentation] マウス精子形成過程のトランスポゾン抑制とRNP制御 (2014)
  • Author(s): 中馬新一郎
  • Organizer: 京都大学霊長類研究所研究会
  • Place of Presentation: 犬山
  • Year and Date: 2014-08-26
  • Invited
• [Presentation] トランスポゾン制御破綻が引き起こす精子形成不全症 (2014)
  • Author(s): 中馬新一郎
  • Organizer: 第61回日本実験動物学会
  • Place of Presentation: 札幌
  • Year and Date: 2014-05-15
  • Invited
• [Presentation] piRNAs in the testis : mammalian tudor genes and germinal granules (2013)
  • Author(s): Chuma S
  • Organizer: International Congress of Andrology
  • Place of Presentation: Melbourne, Australia(招待講演)
  • Year and Date: 2013-02-24